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Assessing Risk Factors of Spoilage and Patulin Contamination in Fruit Products by Paecilomyces niveus- [electronic resource]
Assessing Risk Factors of Spoilage and Patulin Contamination in Fruit Products by Paecilom...
Contents Info
Assessing Risk Factors of Spoilage and Patulin Contamination in Fruit Products by Paecilomyces niveus- [electronic resource]
Material Type  
 단행본
 
0016931633
Date and Time of Latest Transaction  
20240214100055
ISBN  
9798379710750
DDC  
581
Author  
Wang, Tristan Wei.
Title/Author  
Assessing Risk Factors of Spoilage and Patulin Contamination in Fruit Products by Paecilomyces niveus - [electronic resource]
Publish Info  
[S.l.]: : Cornell University., 2023
Publish Info  
Ann Arbor : : ProQuest Dissertations & Theses,, 2023
Material Info  
1 online resource(105 p.)
General Note  
Source: Dissertations Abstracts International, Volume: 84-12, Section: A.
General Note  
Advisor: Hodge, Kathie T.
학위논문주기  
Thesis (Ph.D.)--Cornell University, 2023.
Restrictions on Access Note  
This item must not be sold to any third party vendors.
Abstracts/Etc  
요약In 1909, the first specimen of Paecilomyces niveus Stolk & Samson (Byssochlamys nivea Westling) was isolated from a specimen of Geaster that was stored in alcohol, a prelude to the remarkably persistent properties of this fungus food makers would later come to grips with. In the century since, this heat resistant mold P. niveus has continued to spoil normally shelf-stable fruit products. This is particularly alarming contamination because the fungus produces patulin, a known and regulated mycotoxin. It has been long assumed that P. niveus spoilage inoculum originates from environmental sources like air, soil, and infested equipment, but recently, the fungus was found to cause the postharvest apple disease Paecilomyces rot, capable of infecting and growing in live apple fruits. Processing infected apples can result in highly infested and mycotoxin-contaminated cider, a finding that fundamentally changes how we view sources of P. niveus spoilage inoculum. In the first chapter of this dissertation, I review previous work and new developments regarding research on P. niveus, focusing on 1). The economic impact and extremotolerant properties of this fungus and 2). How the new understanding that P. niveus causes plant disease can drive future research in this field. In chapters two and three, I investigate the host range of Paecilomyces rot and the infection biology of P. niveus. I determined that various rosaceous and citrus fruits are susceptible to infection by P. niveus, and established cultivar-based susceptibility in apple fruits. In chapter 4, I developed species-specific primers based on the patK gene, encoding a 6-methylsalicylic acid synthase, and validated a new qPCR system designed to detect P. niveus in food, fruit, and soil. The qPCR system was used to investigate questions regarding P. niveus disease biology using apple blossom and strawberry bioassays. Lastly, in chapter 5 I assess risk of spoilage and contamination in hard apple cider by four patulin-producing apple pathogens. Our results support my hypothesis that bench-top fermentation does not sufficiently preclude P. niveus spoilage and patulin contamination in finished product. Results from these studies will aid food producers in better understanding and assessing risk factors of P. niveus spoilage and patulin contamination in fruit products.
Subject Added Entry-Topical Term  
Plant pathology.
Subject Added Entry-Topical Term  
Agricultural engineering.
Index Term-Uncontrolled  
Apple cider
Index Term-Uncontrolled  
Paecilomyces niveus
Index Term-Uncontrolled  
Patulin
Index Term-Uncontrolled  
Postharvest disease
Index Term-Uncontrolled  
Spoilage
Added Entry-Corporate Name  
Cornell University Plant Pathology and Plant-Microbe Biology
Host Item Entry  
Dissertations Abstracts International. 84-12A.
Host Item Entry  
Dissertation Abstract International
Electronic Location and Access  
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소장사항  
202402 2024
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