What Goes Right and Wrong During Virus Self-Assembly? Understanding How Bacteriophage MS2 Coat Protein Assembles Around RNA- [electronic resource]
What Goes Right and Wrong During Virus Self-Assembly? Understanding How Bacteriophage MS2 Coat Protein Assembles Around RNA- [electronic resource]
- 자료유형
- 학위논문파일 국외
- 최종처리일시
- 20240214100444
- ISBN
- 9798379605285
- DDC
- 530
- 서명/저자
- What Goes Right and Wrong During Virus Self-Assembly? Understanding How Bacteriophage MS2 Coat Protein Assembles Around RNA - [electronic resource]
- 발행사항
- [S.l.]: : Harvard University., 2023
- 발행사항
- Ann Arbor : : ProQuest Dissertations & Theses,, 2023
- 형태사항
- 1 online resource(87 p.)
- 주기사항
- Source: Dissertations Abstracts International, Volume: 84-12, Section: B.
- 주기사항
- Advisor: Manoharan, Vinothan.
- 학위논문주기
- Thesis (Ph.D.)--Harvard University, 2023.
- 사용제한주기
- This item must not be sold to any third party vendors.
- 초록/해제
- 요약Self-assembly is a vital part of the RNA virus life cycle. The assembly of viral coat proteins around viral RNA occurs both in vivo and in vitro, suggesting that viral capsid assembly may be driven by minimization of free energy. To better understand this process, we study the assembly of bacteriophage MS2 virus-like particles (VLPs as a function of coat-protein concentration, RNA concentration, ionic strength, temperature, and viscosity. We use dynamic light scattering, transmission electron microscopy, and gelelectrophoresis to determinethesize distribution, morphology, and composition of the assembled structures. We show how the morphology of MS2 VLPs varies with these conditions, and we relate these results to a model of assembly involving nucleation and growth.
- 일반주제명
- Physics.
- 일반주제명
- Biophysics.
- 일반주제명
- Biology.
- 키워드
- Virus life cycle
- 키워드
- RNA
- 키워드
- Bacteriophage
- 기타저자
- Harvard University Physics
- 기본자료저록
- Dissertations Abstracts International. 84-12B.
- 기본자료저록
- Dissertation Abstract International
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
