Advances in X-Ray Scattering Interferometry and Liquid Chromatography Coupled Small Angle X-Ray Scattering Towards Exploring DNA-Protein Interactions and Bionanoengineering- [electronic resource]
Advances in X-Ray Scattering Interferometry and Liquid Chromatography Coupled Small Angle X-Ray Scattering Towards Exploring DNA-Protein Interactions and Bionanoengineering- [electronic resource]
- 자료유형
- 학위논문파일 국외
- 최종처리일시
- 20240214100449
- ISBN
- 9798380380980
- DDC
- 574.191
- 서명/저자
- Advances in X-Ray Scattering Interferometry and Liquid Chromatography Coupled Small Angle X-Ray Scattering Towards Exploring DNA-Protein Interactions and Bionanoengineering - [electronic resource]
- 발행사항
- [S.l.]: : University of California, Berkeley., 2023
- 발행사항
- Ann Arbor : : ProQuest Dissertations & Theses,, 2023
- 형태사항
- 1 online resource(336 p.)
- 주기사항
- Source: Dissertations Abstracts International, Volume: 85-03, Section: B.
- 주기사항
- Advisor: Hura, Gregory;Landry, Markita P.
- 학위논문주기
- Thesis (Ph.D.)--University of California, Berkeley, 2023.
- 사용제한주기
- This item must not be sold to any third party vendors.
- 초록/해제
- 요약This dissertation demonstrates the development and application of advanced X-ray scattering techniques toward the exploration of DNA-protein interactions and engineering bionanotechnology. Herein I describe the development of size exclusion chromatography-coupled small-angle X-ray scattering with in-line multi-angle light scattering (SEC-SAXS-MALS) and X-ray scattering interferometry (XSI). SEC-SAXS-MALS is a high-throughput, multimodal structural biology approach with integrated purification whereas XSI leverages the interference pattern between ordered gold nanoparticles (AuNPs) to calculate discrete inter-AuNP distances, effectively turning them into molecular rulers. I demonstrate the application of these techniques in the study of the single-strand break (SSB) DNA-damage repair meiotic recombination 11 (MRE11) nuclease activity, poly(ADP-ribose) polymerase 1 (PARP-1) damage recognition, activation, and inhibition, and the dynamics of the DNA-dependent protein kinase holoenzyme DNA-PK complex. Additionally, I show the versatility of these techniques in bionanoengineering by exploring the surface adsorbed morphology and nanosensing mechanisms of DNA‐functionalized single‐walled carbon nanotubes (ssDNA‐SWCNTs), and the innovation of RuBisCO assemblies. Finally, I show how these techniques provide high-throughput capabilities that enable rapid disaster responses by characterizing the Nsp7, Nsp8, and Nsp12 complexes of SARS-CoV-2 involved in replication, and the exploration of rigid monoclonal antibodies to improve detection of the SARS-CoV-2 nucleocapsid.
- 일반주제명
- Biophysics.
- 일반주제명
- Molecular physics.
- 일반주제명
- Bioengineering.
- 키워드
- SARS-CoV-2
- 키워드
- DNA-PK complex
- 기타저자
- University of California, Berkeley Biophysics
- 기본자료저록
- Dissertations Abstracts International. 85-03B.
- 기본자료저록
- Dissertation Abstract International
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
