Characterization of a Neuroendocrine Network That Coordinates Sugar and Water Ingestion in Drosophila melanogaster- [electronic resource]
Characterization of a Neuroendocrine Network That Coordinates Sugar and Water Ingestion in Drosophila melanogaster- [electronic resource]
- 자료유형
- 학위논문파일 국외
- 최종처리일시
- 20240214100501
- ISBN
- 9798380382458
- DDC
- 616
- 서명/저자
- Characterization of a Neuroendocrine Network That Coordinates Sugar and Water Ingestion in Drosophila melanogaster - [electronic resource]
- 발행사항
- [S.l.]: : University of California, Berkeley., 2023
- 발행사항
- Ann Arbor : : ProQuest Dissertations & Theses,, 2023
- 형태사항
- 1 online resource(146 p.)
- 주기사항
- Source: Dissertations Abstracts International, Volume: 85-03, Section: B.
- 주기사항
- Advisor: Scott, Kristin.
- 학위논문주기
- Thesis (Ph.D.)--University of California, Berkeley, 2023.
- 사용제한주기
- This item must not be sold to any third party vendors.
- 초록/해제
- 요약Consumption of food and water is tightly regulated by the nervous system to maintain internal nutrient homeostasis. Although generally considered independently, interactions between hunger and thirst drives are important to coordinate competing needs. In chapter 1, I provide a review of hunger regulation and thirst regulation in mammals and Drosophila melanogaster. I then discuss the neural and hormonal coordination of hunger and thirst in both mammals and D. melanogaster.In Drosophila, four neurons called the Interoceptive Subesophageal zone Neurons (ISNs) respond to intrinsic hunger and thirst signals to oppositely regulate sucrose and water ingestion. In chapter 2, I characterize the neural circuit downstream of the ISNs. This work is presented in the form of a preprint first author manuscript. Using the fly brain connectome, genetic tools, behavioral assays, and functional imaging, I show, together with co-authors Gina Pontes, Nicholas Jourjine and Alexander Del Toro, that the ISNs modulate a peptidergic network of neurons. These include a novel cell type Bilateral T shaped neuron (BiT), insulin producing cells (IPC), crustacean cardioactive peptide (CCAP) neurons, and CCHamide-2 receptor isoform RA (CCHa2R-RA) neurons. These neurons contribute differentially to ingestion of sugar and water, with BiT, IPCs and CCAP neurons oppositely regulating sugar and water ingestion, and CCHa2R-RA neurons modulating only water ingestion. Thus, the decision to consume sugar or water occurs via regulation of a broad peptidergic network that integrates internal signals of nutritional state to generate nutrient-specific ingestion.In chapter 3, I characterize other neurons that are involved in sugar and/or water ingestion. Using a computational approach to identify neurons in close proximity, genetic manipulation, behavioral assays, and in vivo functional imaging, I characterized several neurons involved in sugar and/or water ingestion regulation. Aster, bidirectionally regulates sugar and water ingestion just as the ISNs, Horseshoe, decreases both sugar and water ingestion, and Cowboy likely promotes sugar ingestion. In vivo functional connectivity experiments revealed that they were not downstream of the ISNs, however, Aster, possibly Horseshoe, and another cell type Gallinule are downstream of sensory neurons. Thus, I have identified another cell type that bidirectionally regulates sugar and water ingestion, two cell types that modulate feeding, and a cell type that likely conveys gustatory information to memory centers in the fly brain.In chapter 4, I describe with co-authors Zoila Alvarez-Aponte, Rachel Brem, Diana Bautista and Denzil Streete, the development and implementation of the Inclusive Excellence in Quals Prep (IEQP) program. This program was designed to provide mentorship, community, and academic support for students from diverse backgrounds as they prepared for their QE. The main components for IEQP program included pairing students with graduate student mentors, academic and wellness workshops, and community building events. This program was first implemented on a pilot cohort of 11 graduate students. After program evaluation, the most significant component of the program was peer mentorship. After program completion, students' perception of their preparedness, QE-related skills, the support received from their advisors, and the agency they felt over their proposed work increased.In the final part of this dissertation, chapter 5, I summarize the main findings of this dissertation and propose future directions for exploration.
- 일반주제명
- Neurosciences.
- 일반주제명
- Cellular biology.
- 일반주제명
- Molecular biology.
- 키워드
- Feeding
- 키워드
- Homeostasis
- 키워드
- Hunger
- 기타저자
- University of California, Berkeley Molecular & Cell Biology
- 기본자료저록
- Dissertations Abstracts International. 85-03B.
- 기본자료저록
- Dissertation Abstract International
- 전자적 위치 및 접속
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