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Development of Electrochemical Sensors for Analytical and Biomedical Applications- [electronic resource]
Development of Electrochemical Sensors for Analytical and Biomedical Applications- [electronic resource]
상세정보
- 자료유형
- 학위논문파일 국외
- 최종처리일시
- 20240214095830
- ISBN
- 9798380392587
- DDC
- 543
- 저자명
- Chen, Xin V.
- 서명/저자
- Development of Electrochemical Sensors for Analytical and Biomedical Applications - [electronic resource]
- 발행사항
- [S.l.]: : University of Minnesota., 2019
- 발행사항
- Ann Arbor : : ProQuest Dissertations & Theses,, 2019
- 형태사항
- 1 online resource(286 p.)
- 주기사항
- Source: Dissertations Abstracts International, Volume: 85-03, Section: B.
- 주기사항
- Advisor: Buhlmann, Philippe.
- 학위논문주기
- Thesis (Ph.D.)--University of Minnesota, 2019.
- 사용제한주기
- This item must not be sold to any third party vendors.
- 사용제한주기
- This item must not be added to any third party search indexes.
- 초록/해제
- 요약The focus of this dissertation is on two main topics: the development of chemical sensors with reduced biofouling for applications in biological samples (Chapter I-II), and the development of chemical sensors with improved biocompatibility (Chapter III-V).Conventional polymeric membrane-based ion-selective electrodes (ISEs) rely on plasticized poly(vinyl chloride) (PVC) as sensor membranes. The plasticizers that solubilize PVC backbone-a prerequisite for PVC-phase ISEs-leach out gradually, resulting in a limited sensor lifetime. Polar groups in the plasticizer may also lower the sensor selectivity. To improve selectivity and expand working ranges, fluorous-phase ISEs relying on nonpolar perfluorinated compounds as sensing membrane were developed. A novel fluorophilic ionophore was synthesized and used to make ionophore-doped fluorous-phase ISEs with Nernstian responses and an optimal working range centered around neutral pH-suitable for most biological samples. The reproducibility of fluorous-phase ISEs was enhanced by a new electrode body design. Importantly, fluorous-phase ISEs maintained their excellent selectivity after prolonged exposure in serum whereas PVC-phase ISEs lost selectivity considerably. Insights were also obtained on the optimal ionophore-to-ionic site ratio.To improve biocompatibility, silicone-based reference and ion-selective electrodes were developed to eliminate plasticizers. Reference electrodes doped with several ionic liquids showed sample-independent and long-term stable potentials in artificial blood electrolytes and serum samples. Potassium-selective silicone-based ISEs developed with two ionophores and two silicones showed Nernstian responses and good selectivities. In an attempt to prevent leaching of ionophores from ISE membrane into samples, a well-known potassium ionophore was covalently attached to silicone membranes. Miniaturized microelectrodes suitable for implantable devices were also developed based on this platform. In a similar effort, plasticizer-free polymethacrylate-based ISEs exhibited Nernstian responses to pH and selectivities comparable to PVC-phase ISEs. To further improve biocompatibility for applications in the pharmaceutical and food industries, either an ionophore or ionic site or both were covalently attached to sensor membranes. Sensors with either ionophore or ionic site attached provided similar good characteristics whereas when both were attached, Nernstian responses were not found consistently. Furthermore, heating experiments showed that sensors exposed to 90 ˚C heating maintained good selectivity.
- 일반주제명
- Analytical chemistry.
- 일반주제명
- Physical chemistry.
- 일반주제명
- Biomedical engineering.
- 키워드
- Anti-Biofouling
- 키워드
- Biocompatibility
- 키워드
- Potentiometry
- 기타저자
- University of Minnesota Chemistry
- 기본자료저록
- Dissertations Abstracts International. 85-03B.
- 기본자료저록
- Dissertation Abstract International
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■00520240214095830
■006m o d
■007cr#unu||||||||
■020 ▼a9798380392587
■035 ▼a(MiAaPQ)AAI22583423
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a543
■1001 ▼aChen, Xin V.
■24510▼aDevelopment of Electrochemical Sensors for Analytical and Biomedical Applications▼h[electronic resource]
■260 ▼a[S.l.]:▼bUniversity of Minnesota. ▼c2019
■260 1▼aAnn Arbor :▼bProQuest Dissertations & Theses, ▼c2019
■300 ▼a1 online resource(286 p.)
■500 ▼aSource: Dissertations Abstracts International, Volume: 85-03, Section: B.
■500 ▼aAdvisor: Buhlmann, Philippe.
■5021 ▼aThesis (Ph.D.)--University of Minnesota, 2019.
■506 ▼aThis item must not be sold to any third party vendors.
■506 ▼aThis item must not be added to any third party search indexes.
■520 ▼aThe focus of this dissertation is on two main topics: the development of chemical sensors with reduced biofouling for applications in biological samples (Chapter I-II), and the development of chemical sensors with improved biocompatibility (Chapter III-V).Conventional polymeric membrane-based ion-selective electrodes (ISEs) rely on plasticized poly(vinyl chloride) (PVC) as sensor membranes. The plasticizers that solubilize PVC backbone-a prerequisite for PVC-phase ISEs-leach out gradually, resulting in a limited sensor lifetime. Polar groups in the plasticizer may also lower the sensor selectivity. To improve selectivity and expand working ranges, fluorous-phase ISEs relying on nonpolar perfluorinated compounds as sensing membrane were developed. A novel fluorophilic ionophore was synthesized and used to make ionophore-doped fluorous-phase ISEs with Nernstian responses and an optimal working range centered around neutral pH-suitable for most biological samples. The reproducibility of fluorous-phase ISEs was enhanced by a new electrode body design. Importantly, fluorous-phase ISEs maintained their excellent selectivity after prolonged exposure in serum whereas PVC-phase ISEs lost selectivity considerably. Insights were also obtained on the optimal ionophore-to-ionic site ratio.To improve biocompatibility, silicone-based reference and ion-selective electrodes were developed to eliminate plasticizers. Reference electrodes doped with several ionic liquids showed sample-independent and long-term stable potentials in artificial blood electrolytes and serum samples. Potassium-selective silicone-based ISEs developed with two ionophores and two silicones showed Nernstian responses and good selectivities. In an attempt to prevent leaching of ionophores from ISE membrane into samples, a well-known potassium ionophore was covalently attached to silicone membranes. Miniaturized microelectrodes suitable for implantable devices were also developed based on this platform. In a similar effort, plasticizer-free polymethacrylate-based ISEs exhibited Nernstian responses to pH and selectivities comparable to PVC-phase ISEs. To further improve biocompatibility for applications in the pharmaceutical and food industries, either an ionophore or ionic site or both were covalently attached to sensor membranes. Sensors with either ionophore or ionic site attached provided similar good characteristics whereas when both were attached, Nernstian responses were not found consistently. Furthermore, heating experiments showed that sensors exposed to 90 ˚C heating maintained good selectivity.
■590 ▼aSchool code: 0130.
■650 4▼aAnalytical chemistry.
■650 4▼aPhysical chemistry.
■650 4▼aBiomedical engineering.
■653 ▼aAnti-Biofouling
■653 ▼aBiocompatibility
■653 ▼aIon-selective electrodes
■653 ▼aPotentiometry
■653 ▼aReference electrode
■690 ▼a0486
■690 ▼a0494
■690 ▼a0541
■71020▼aUniversity of Minnesota▼bChemistry.
■7730 ▼tDissertations Abstracts International▼g85-03B.
■773 ▼tDissertation Abstract International
■790 ▼a0130
■791 ▼aPh.D.
■792 ▼a2019
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T16930905▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
■980 ▼a202402▼f2024
