서브메뉴
검색
Integrating DNA Logic Circuits and Isothermal Amplification Methods: Novel Tools for Single-Cell Transcriptional Profiling and Diagnostics- [electronic resource]
Integrating DNA Logic Circuits and Isothermal Amplification Methods: Novel Tools for Single-Cell Transcriptional Profiling and Diagnostics- [electronic resource]
상세정보
- 자료유형
- 학위논문파일 국외
- 최종처리일시
- 20240214095834
- ISBN
- 9798380583619
- DDC
- 574
- 저자명
- Hyman, Leland B.
- 서명/저자
- Integrating DNA Logic Circuits and Isothermal Amplification Methods: Novel Tools for Single-Cell Transcriptional Profiling and Diagnostics - [electronic resource]
- 발행사항
- [S.l.]: : The University of Wisconsin - Madison., 2020
- 발행사항
- Ann Arbor : : ProQuest Dissertations & Theses,, 2020
- 형태사항
- 1 online resource(148 p.)
- 주기사항
- Source: Dissertations Abstracts International, Volume: 85-04, Section: B.
- 주기사항
- Advisor: Romero, Philip A.
- 학위논문주기
- Thesis (Ph.D.)--The University of Wisconsin - Madison, 2020.
- 사용제한주기
- This item must not be sold to any third party vendors.
- 초록/해제
- 요약Intracellular heterogeneity affects the human body's response to drug treatment, pathogen infection, and disease processes. Methods to characterize single-cell transcriptional activities are currently expensive, laborious, and limited in cell throughput. By leveraging recent advances including droplet microfluidics, Loop-mediated Isothermal Amplification (LAMP), and DNA logic techniques, we have developed a simple, inexpensive, and high-throughput transcriptional profiling method: Single-cell Nucleic Acid Profiling in Droplets (SNAPD). We showed that this unprecedentedly simple workflow can analyze target gene expression in cell mixtures with incredible sensitivity and selectivity. We further enhanced SNAPD by developing a novel library of DNA logic gates which integrate with LAMP. We have demonstrated that SNAPD is an advantageous technique to study rare and mixed cell populations and could be powerful in many biological settings. We also applied these DNA logic principles to develop a novel method for accurate and reliable Single Nucleotide Polymorphism (SNP) detection. This method could be integrated with SNAPD for single-cell SNP assays, or applied directly to point-of-care diagnostics. In this work, we have developed two approaches which leverage LAMP and molecular biology techniques to create powerful research tools, proving their utility in oncological and diagnostic applications. Alone, each of these advances opens avenues for future work in diverse biological applications and diagnostics. Combined, they may comprise a single-cell SNP profiling method of unprecedented simplicity and performance.
- 일반주제명
- Cellular biology.
- 일반주제명
- Biomedical engineering.
- 일반주제명
- Biochemistry.
- 일반주제명
- Molecular biology.
- 일반주제명
- Genetics.
- 키워드
- Microfluidics
- 기타저자
- The University of Wisconsin - Madison Cellular & Molecular Bio - AG
- 기본자료저록
- Dissertations Abstracts International. 85-04B.
- 기본자료저록
- Dissertation Abstract International
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
008240612s2020 us |||||||||||||||c||eng d■001000016930917
■00520240214095834
■006m o d
■007cr#unu||||||||
■020 ▼a9798380583619
■035 ▼a(MiAaPQ)AAI28256982
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574
■1001 ▼aHyman, Leland B.
■24510▼aIntegrating DNA Logic Circuits and Isothermal Amplification Methods: Novel Tools for Single-Cell Transcriptional Profiling and Diagnostics▼h[electronic resource]
■260 ▼a[S.l.]:▼bThe University of Wisconsin - Madison. ▼c2020
■260 1▼aAnn Arbor :▼bProQuest Dissertations & Theses, ▼c2020
■300 ▼a1 online resource(148 p.)
■500 ▼aSource: Dissertations Abstracts International, Volume: 85-04, Section: B.
■500 ▼aAdvisor: Romero, Philip A.
■5021 ▼aThesis (Ph.D.)--The University of Wisconsin - Madison, 2020.
■506 ▼aThis item must not be sold to any third party vendors.
■520 ▼aIntracellular heterogeneity affects the human body's response to drug treatment, pathogen infection, and disease processes. Methods to characterize single-cell transcriptional activities are currently expensive, laborious, and limited in cell throughput. By leveraging recent advances including droplet microfluidics, Loop-mediated Isothermal Amplification (LAMP), and DNA logic techniques, we have developed a simple, inexpensive, and high-throughput transcriptional profiling method: Single-cell Nucleic Acid Profiling in Droplets (SNAPD). We showed that this unprecedentedly simple workflow can analyze target gene expression in cell mixtures with incredible sensitivity and selectivity. We further enhanced SNAPD by developing a novel library of DNA logic gates which integrate with LAMP. We have demonstrated that SNAPD is an advantageous technique to study rare and mixed cell populations and could be powerful in many biological settings. We also applied these DNA logic principles to develop a novel method for accurate and reliable Single Nucleotide Polymorphism (SNP) detection. This method could be integrated with SNAPD for single-cell SNP assays, or applied directly to point-of-care diagnostics. In this work, we have developed two approaches which leverage LAMP and molecular biology techniques to create powerful research tools, proving their utility in oncological and diagnostic applications. Alone, each of these advances opens avenues for future work in diverse biological applications and diagnostics. Combined, they may comprise a single-cell SNP profiling method of unprecedented simplicity and performance.
■590 ▼aSchool code: 0262.
■650 4▼aCellular biology.
■650 4▼aBiomedical engineering.
■650 4▼aBiochemistry.
■650 4▼aMolecular biology.
■650 4▼aGenetics.
■653 ▼aLoop-mediated Isothermal Amplification
■653 ▼aMicrofluidics
■653 ▼aPoint-of-care diagnostics
■653 ▼aSingle-cell transcriptomics
■653 ▼aDNA logic techniques
■690 ▼a0379
■690 ▼a0541
■690 ▼a0487
■690 ▼a0369
■690 ▼a0307
■71020▼aThe University of Wisconsin - Madison▼bCellular & Molecular Bio - AG.
■7730 ▼tDissertations Abstracts International▼g85-04B.
■773 ▼tDissertation Abstract International
■790 ▼a0262
■791 ▼aPh.D.
■792 ▼a2020
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T16930917▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
■980 ▼a202402▼f2024
