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The Identification of Sequence Features and Regulators of Co-Translationally Decayed Messenger RNA- [electronic resource]
The Identification of Sequence Features and Regulators of Co-Translationally Decayed Messenger RNA- [electronic resource]
상세정보
- 자료유형
- 학위논문파일 국외
- 최종처리일시
- 20240214101257
- ISBN
- 9798380388412
- DDC
- 574
- 저자명
- Guo, Rong.
- 서명/저자
- The Identification of Sequence Features and Regulators of Co-Translationally Decayed Messenger RNA - [electronic resource]
- 발행사항
- [S.l.]: : University of Pennsylvania., 2023
- 발행사항
- Ann Arbor : : ProQuest Dissertations & Theses,, 2023
- 형태사항
- 1 online resource(126 p.)
- 주기사항
- Source: Dissertations Abstracts International, Volume: 85-03, Section: B.
- 주기사항
- Advisor: Gregory, Brian D.
- 학위논문주기
- Thesis (Ph.D.)--University of Pennsylvania, 2023.
- 사용제한주기
- This item must not be sold to any third party vendors.
- 초록/해제
- 요약Despite the prevailing belief that translating mRNAs are excluded from degradation, co-translational mRNA decay (CTRD), a mechanism that degrades mRNAs associated with actively translating ribosomes, has been identified in Arabidopsis, rice, and soybean as well as in yeast and humans. However, it is not known whether this decay mechanism is conserved in other plant species. Moreover, the regulation and physiological significance of this decay mechanism is also less well known. In this thesis, I describe research where I found that co-translational mRNA decay is conserved in at least 10 angiosperm species and present in many tissues as well as among various growth stages and environmental conditions. Additionally, I discovered that the co-translationally decayed mRNAs in every species consistently harbor sequence features linking to low translation activity. In terms of CTRD regulation, I have identified Arabidopsis Pelota and Hbs1 as suppressors of CTRD in vivo. Lastly, I demonstrated that Pelota and Hbs1 loss of function mutants (pel and hbs1, respectively) have lower germination rates, which might be due to embryo development defects caused by increased levels co-translational mRNA decay induced down-regulation of relevant transcripts in these mutant backgrounds. These results indicate that the level of co-translational mRNA decay needs to be tightly controlled to ensure normal physiological functions in plant species. Taken together, my study expanded the general knowledge concerning the regulation and physiological significance of CTRD in plants as well as presented the sequence features associated with the co-translationally decayed mRNAs, which provided insights into the possible triggering mechanism for this decay mechanism.
- 일반주제명
- Biology.
- 일반주제명
- Bioinformatics.
- 일반주제명
- Molecular biology.
- 키워드
- Plant species
- 기타저자
- University of Pennsylvania Biology
- 기본자료저록
- Dissertations Abstracts International. 85-03B.
- 기본자료저록
- Dissertation Abstract International
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■00520240214101257
■006m o d
■007cr#unu||||||||
■020 ▼a9798380388412
■035 ▼a(MiAaPQ)AAI30530758
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574
■1001 ▼aGuo, Rong.
■24510▼aThe Identification of Sequence Features and Regulators of Co-Translationally Decayed Messenger RNA▼h[electronic resource]
■260 ▼a[S.l.]:▼bUniversity of Pennsylvania. ▼c2023
■260 1▼aAnn Arbor :▼bProQuest Dissertations & Theses, ▼c2023
■300 ▼a1 online resource(126 p.)
■500 ▼aSource: Dissertations Abstracts International, Volume: 85-03, Section: B.
■500 ▼aAdvisor: Gregory, Brian D.
■5021 ▼aThesis (Ph.D.)--University of Pennsylvania, 2023.
■506 ▼aThis item must not be sold to any third party vendors.
■520 ▼aDespite the prevailing belief that translating mRNAs are excluded from degradation, co-translational mRNA decay (CTRD), a mechanism that degrades mRNAs associated with actively translating ribosomes, has been identified in Arabidopsis, rice, and soybean as well as in yeast and humans. However, it is not known whether this decay mechanism is conserved in other plant species. Moreover, the regulation and physiological significance of this decay mechanism is also less well known. In this thesis, I describe research where I found that co-translational mRNA decay is conserved in at least 10 angiosperm species and present in many tissues as well as among various growth stages and environmental conditions. Additionally, I discovered that the co-translationally decayed mRNAs in every species consistently harbor sequence features linking to low translation activity. In terms of CTRD regulation, I have identified Arabidopsis Pelota and Hbs1 as suppressors of CTRD in vivo. Lastly, I demonstrated that Pelota and Hbs1 loss of function mutants (pel and hbs1, respectively) have lower germination rates, which might be due to embryo development defects caused by increased levels co-translational mRNA decay induced down-regulation of relevant transcripts in these mutant backgrounds. These results indicate that the level of co-translational mRNA decay needs to be tightly controlled to ensure normal physiological functions in plant species. Taken together, my study expanded the general knowledge concerning the regulation and physiological significance of CTRD in plants as well as presented the sequence features associated with the co-translationally decayed mRNAs, which provided insights into the possible triggering mechanism for this decay mechanism.
■590 ▼aSchool code: 0175.
■650 4▼aBiology.
■650 4▼aBioinformatics.
■650 4▼aMolecular biology.
■653 ▼aTranslating ribosomes
■653 ▼aCo-translational mRNA
■653 ▼aPhysiological functions
■653 ▼aEmbryo development
■653 ▼aPlant species
■690 ▼a0306
■690 ▼a0715
■690 ▼a0307
■71020▼aUniversity of Pennsylvania▼bBiology.
■7730 ▼tDissertations Abstracts International▼g85-03B.
■773 ▼tDissertation Abstract International
■790 ▼a0175
■791 ▼aPh.D.
■792 ▼a2023
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T16933529▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
■980 ▼a202402▼f2024
