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Long-Term Impacts of Uterine Infection on Fertility in Dairy Cows- [electronic resource]
![Long-Term Impacts of Uterine Infection on Fertility in Dairy Cows - [electronic resource]](/Users/Baul/Images/book.png)
Long-Term Impacts of Uterine Infection on Fertility in Dairy Cows- [electronic resource]
상세정보
- 자료유형
- 학위논문파일 국외
- 최종처리일시
- 20240214095838
- ISBN
- 9798380210645
- DDC
- 636
- 서명/저자
- Long-Term Impacts of Uterine Infection on Fertility in Dairy Cows - [electronic resource]
- 발행사항
- [S.l.]: : University of Florida., 2021
- 발행사항
- Ann Arbor : : ProQuest Dissertations & Theses,, 2021
- 형태사항
- 1 online resource(278 p.)
- 주기사항
- Source: Dissertations Abstracts International, Volume: 85-03, Section: B.
- 주기사항
- Advisor: Bromfield, John J.
- 학위논문주기
- Thesis (Ph.D.)--University of Florida, 2021.
- 사용제한주기
- This item must not be sold to any third party vendors.
- 초록/해제
- 요약In cows, uterine disease is associated with infertility after disease resolution. Cows with uterine disease have reduced estradiol secretion, decreased conception rates, and increased pregnancy loss. The mechanisms responsible for this persistent infertility are unknown. The bacterial component, lipopolysaccharide (LPS), accumulates in the follicular fluid of cows with uterine disease, potentially altering the follicular environment in which oocytes develop. In vitro, LPS decreases oocyte competence and reduces CYP19A1 expression and estradiol production in granulosa cells. Embryo transfer does not restore fertility in cows after uterine disease, suggesting uterine disease impacts the endometrium.I hypothesized that induced uterine disease reduces the developmental competence of oocytes and alters the endometrial signature of pregnancy. Furthermore, I hypothesized that CCAAT/Enhancer-binding protein (CEBPβ), which regulates CYP19A1 expression, mediates LPS-induced downregulation of CYP19A1 expression and estradiol production in granulosa cells.Cows received an intrauterine infusion of pathogenic bacteria or vehicle to induce uterine disease. Subsequently, oocytes were collected and subjected to in vitro fertilization and embryo culture. Bacterial infusion resulted in reduced embryo development compared to vehicle infused controls.RNA sequencing was performed on endometrial tissue on day 16 after insemination in cows that received an intrauterine bacterial infusion. The endometrial signature of pregnancy in cows following bacterial infusion was compared to the endometrial signature of pregnancy previously reported in healthy cows. Compared to healthy cows, the endometrial signature of pregnancy in cows following bacterial infusion showed a dysregulation of pathways related to inflammation and immune function, including IL-7, TLR, and iNOS signaling.Lastly, I demonstrated that LPS reduces estradiol secretion in bovine granulosa cells from small/medium and large follicles. Interestingly the data suggests that LPS mediated changes to CYP19A1 expression and CEBPβ nuclear localization in granulosa cells is dependent on follicle size.In summary, intrauterine bacterial infusion reduces oocyte competence and results in a unique endometrial transcriptome of cows that fail to become pregnant, while granulosa cells exposed to LPS alter CEBPβ signaling and reduce estradiol secretion. Collectively, these studies illuminate potential mechanisms in the ovary and endometrium for the persistent infertility observed in dairy cows after the resolution of uterine disease.
- 일반주제명
- Animal sciences.
- 일반주제명
- Cellular biology.
- 키워드
- Infertility
- 키워드
- Inflammation
- 키워드
- Ovary
- 키워드
- Uterus
- 기타저자
- University of Florida Animal Molecular and Cellular Biology
- 기본자료저록
- Dissertations Abstracts International. 85-03B.
- 기본자료저록
- Dissertation Abstract International
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■020 ▼a9798380210645
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■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a636
■1001 ▼aDickson, Mackenzie Joy.
■24510▼aLong-Term Impacts of Uterine Infection on Fertility in Dairy Cows▼h[electronic resource]
■260 ▼a[S.l.]:▼bUniversity of Florida. ▼c2021
■260 1▼aAnn Arbor :▼bProQuest Dissertations & Theses, ▼c2021
■300 ▼a1 online resource(278 p.)
■500 ▼aSource: Dissertations Abstracts International, Volume: 85-03, Section: B.
■500 ▼aAdvisor: Bromfield, John J.
■5021 ▼aThesis (Ph.D.)--University of Florida, 2021.
■506 ▼aThis item must not be sold to any third party vendors.
■520 ▼aIn cows, uterine disease is associated with infertility after disease resolution. Cows with uterine disease have reduced estradiol secretion, decreased conception rates, and increased pregnancy loss. The mechanisms responsible for this persistent infertility are unknown. The bacterial component, lipopolysaccharide (LPS), accumulates in the follicular fluid of cows with uterine disease, potentially altering the follicular environment in which oocytes develop. In vitro, LPS decreases oocyte competence and reduces CYP19A1 expression and estradiol production in granulosa cells. Embryo transfer does not restore fertility in cows after uterine disease, suggesting uterine disease impacts the endometrium.I hypothesized that induced uterine disease reduces the developmental competence of oocytes and alters the endometrial signature of pregnancy. Furthermore, I hypothesized that CCAAT/Enhancer-binding protein (CEBPβ), which regulates CYP19A1 expression, mediates LPS-induced downregulation of CYP19A1 expression and estradiol production in granulosa cells.Cows received an intrauterine infusion of pathogenic bacteria or vehicle to induce uterine disease. Subsequently, oocytes were collected and subjected to in vitro fertilization and embryo culture. Bacterial infusion resulted in reduced embryo development compared to vehicle infused controls.RNA sequencing was performed on endometrial tissue on day 16 after insemination in cows that received an intrauterine bacterial infusion. The endometrial signature of pregnancy in cows following bacterial infusion was compared to the endometrial signature of pregnancy previously reported in healthy cows. Compared to healthy cows, the endometrial signature of pregnancy in cows following bacterial infusion showed a dysregulation of pathways related to inflammation and immune function, including IL-7, TLR, and iNOS signaling.Lastly, I demonstrated that LPS reduces estradiol secretion in bovine granulosa cells from small/medium and large follicles. Interestingly the data suggests that LPS mediated changes to CYP19A1 expression and CEBPβ nuclear localization in granulosa cells is dependent on follicle size.In summary, intrauterine bacterial infusion reduces oocyte competence and results in a unique endometrial transcriptome of cows that fail to become pregnant, while granulosa cells exposed to LPS alter CEBPβ signaling and reduce estradiol secretion. Collectively, these studies illuminate potential mechanisms in the ovary and endometrium for the persistent infertility observed in dairy cows after the resolution of uterine disease.
■590 ▼aSchool code: 0070.
■650 4▼aAnimal sciences.
■650 4▼aCellular biology.
■653 ▼aBovine granulosa cells
■653 ▼aInfertility
■653 ▼aInflammation
■653 ▼aOvary
■653 ▼aUterus
■690 ▼a0475
■690 ▼a0379
■690 ▼a0778
■71020▼aUniversity of Florida▼bAnimal Molecular and Cellular Biology.
■7730 ▼tDissertations Abstracts International▼g85-03B.
■773 ▼tDissertation Abstract International
■790 ▼a0070
■791 ▼aPh.D.
■792 ▼a2021
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T16930934▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
■980 ▼a202402▼f2024
