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Assessing Risk Factors of Spoilage and Patulin Contamination in Fruit Products by Paecilomyces niveus- [electronic resource]
Assessing Risk Factors of Spoilage and Patulin Contamination in Fruit Products by Paecilom...
Assessing Risk Factors of Spoilage and Patulin Contamination in Fruit Products by Paecilomyces niveus- [electronic resource]

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자료유형  
 학위논문파일 국외
최종처리일시  
20240214100055
ISBN  
9798379710750
DDC  
581
저자명  
Wang, Tristan Wei.
서명/저자  
Assessing Risk Factors of Spoilage and Patulin Contamination in Fruit Products by Paecilomyces niveus - [electronic resource]
발행사항  
[S.l.]: : Cornell University., 2023
발행사항  
Ann Arbor : : ProQuest Dissertations & Theses,, 2023
형태사항  
1 online resource(105 p.)
주기사항  
Source: Dissertations Abstracts International, Volume: 84-12, Section: A.
주기사항  
Advisor: Hodge, Kathie T.
학위논문주기  
Thesis (Ph.D.)--Cornell University, 2023.
사용제한주기  
This item must not be sold to any third party vendors.
초록/해제  
요약In 1909, the first specimen of Paecilomyces niveus Stolk & Samson (Byssochlamys nivea Westling) was isolated from a specimen of Geaster that was stored in alcohol, a prelude to the remarkably persistent properties of this fungus food makers would later come to grips with. In the century since, this heat resistant mold P. niveus has continued to spoil normally shelf-stable fruit products. This is particularly alarming contamination because the fungus produces patulin, a known and regulated mycotoxin. It has been long assumed that P. niveus spoilage inoculum originates from environmental sources like air, soil, and infested equipment, but recently, the fungus was found to cause the postharvest apple disease Paecilomyces rot, capable of infecting and growing in live apple fruits. Processing infected apples can result in highly infested and mycotoxin-contaminated cider, a finding that fundamentally changes how we view sources of P. niveus spoilage inoculum. In the first chapter of this dissertation, I review previous work and new developments regarding research on P. niveus, focusing on 1). The economic impact and extremotolerant properties of this fungus and 2). How the new understanding that P. niveus causes plant disease can drive future research in this field. In chapters two and three, I investigate the host range of Paecilomyces rot and the infection biology of P. niveus. I determined that various rosaceous and citrus fruits are susceptible to infection by P. niveus, and established cultivar-based susceptibility in apple fruits. In chapter 4, I developed species-specific primers based on the patK gene, encoding a 6-methylsalicylic acid synthase, and validated a new qPCR system designed to detect P. niveus in food, fruit, and soil. The qPCR system was used to investigate questions regarding P. niveus disease biology using apple blossom and strawberry bioassays. Lastly, in chapter 5 I assess risk of spoilage and contamination in hard apple cider by four patulin-producing apple pathogens. Our results support my hypothesis that bench-top fermentation does not sufficiently preclude P. niveus spoilage and patulin contamination in finished product. Results from these studies will aid food producers in better understanding and assessing risk factors of P. niveus spoilage and patulin contamination in fruit products.
일반주제명  
Plant pathology.
일반주제명  
Agricultural engineering.
키워드  
Apple cider
키워드  
Paecilomyces niveus
키워드  
Patulin
키워드  
Postharvest disease
키워드  
Spoilage
기타저자  
Cornell University Plant Pathology and Plant-Microbe Biology
기본자료저록  
Dissertations Abstracts International. 84-12A.
기본자료저록  
Dissertation Abstract International
전자적 위치 및 접속  
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MARC

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■00520240214100055
■006m          o    d                
■007cr#unu||||||||
■020    ▼a9798379710750
■035    ▼a(MiAaPQ)AAI30317967
■040    ▼aMiAaPQ▼cMiAaPQ
■0820  ▼a581
■1001  ▼aWang,  Tristan  Wei.▼0(orcid)0000-0001-6889-2453
■24510▼aAssessing  Risk  Factors  of  Spoilage  and  Patulin  Contamination  in  Fruit  Products  by  Paecilomyces  niveus▼h[electronic  resource]
■260    ▼a[S.l.]:▼bCornell  University.  ▼c2023
■260  1▼aAnn  Arbor  :▼bProQuest  Dissertations  &  Theses,  ▼c2023
■300    ▼a1  online  resource(105  p.)
■500    ▼aSource:  Dissertations  Abstracts  International,  Volume:  84-12,  Section:  A.
■500    ▼aAdvisor:  Hodge,  Kathie  T.
■5021  ▼aThesis  (Ph.D.)--Cornell  University,  2023.
■506    ▼aThis  item  must  not  be  sold  to  any  third  party  vendors.
■520    ▼aIn  1909,  the  first  specimen  of  Paecilomyces  niveus  Stolk  &  Samson  (Byssochlamys  nivea  Westling)  was  isolated  from  a  specimen  of  Geaster  that  was  stored  in  alcohol,  a  prelude  to  the  remarkably  persistent  properties  of  this  fungus  food  makers  would  later  come  to  grips  with.  In  the  century  since,  this  heat  resistant  mold  P.  niveus  has  continued  to  spoil  normally  shelf-stable  fruit  products.  This  is  particularly  alarming  contamination  because  the  fungus  produces  patulin,  a  known  and  regulated  mycotoxin.  It  has  been  long  assumed  that  P.  niveus  spoilage  inoculum  originates  from  environmental  sources  like  air,  soil,  and  infested  equipment,  but  recently,  the  fungus  was  found  to  cause  the  postharvest  apple  disease  Paecilomyces  rot,  capable  of  infecting  and  growing  in  live  apple  fruits.  Processing  infected  apples  can  result  in  highly  infested  and  mycotoxin-contaminated  cider,  a  finding  that  fundamentally  changes  how  we  view  sources  of  P.  niveus  spoilage  inoculum. In  the  first  chapter  of  this  dissertation,  I  review  previous  work  and  new  developments  regarding  research  on  P.  niveus,  focusing  on  1).  The  economic  impact  and  extremotolerant  properties  of  this  fungus  and  2).  How  the  new  understanding  that  P.  niveus  causes  plant  disease  can  drive  future  research  in  this  field.  In  chapters  two  and  three,  I  investigate  the  host  range  of  Paecilomyces  rot  and  the  infection  biology  of  P.  niveus.  I  determined  that  various  rosaceous  and  citrus  fruits  are  susceptible  to  infection  by  P.  niveus,  and  established  cultivar-based  susceptibility  in  apple  fruits.  In chapter  4,  I  developed  species-specific  primers  based  on  the  patK  gene,  encoding  a  6-methylsalicylic  acid  synthase,  and  validated  a  new  qPCR  system  designed  to  detect  P.  niveus  in  food,  fruit,  and  soil.  The  qPCR  system  was  used  to  investigate  questions  regarding  P.  niveus  disease  biology  using  apple  blossom  and  strawberry  bioassays.  Lastly,  in  chapter  5  I  assess  risk  of  spoilage  and  contamination  in  hard  apple  cider  by  four  patulin-producing  apple  pathogens.  Our  results  support  my  hypothesis  that  bench-top  fermentation  does  not  sufficiently  preclude  P.  niveus  spoilage  and  patulin  contamination  in  finished  product.  Results  from  these  studies  will  aid  food  producers  in  better  understanding  and  assessing  risk  factors  of  P.  niveus  spoilage  and  patulin  contamination  in  fruit  products.
■590    ▼aSchool  code:  0058.
■650  4▼aPlant  pathology.
■650  4▼aAgricultural  engineering.
■653    ▼aApple  cider
■653    ▼aPaecilomyces  niveus
■653    ▼aPatulin
■653    ▼aPostharvest  disease
■653    ▼aSpoilage
■690    ▼a0480
■690    ▼a0503
■690    ▼a0539
■71020▼aCornell  University▼bPlant  Pathology  and  Plant-Microbe  Biology.
■7730  ▼tDissertations  Abstracts  International▼g84-12A.
■773    ▼tDissertation  Abstract  International
■790    ▼a0058
■791    ▼aPh.D.
■792    ▼a2023
■793    ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T16931633▼nKERIS▼z이  자료의  원문은  한국교육학술정보원에서  제공합니다.
■980    ▼a202402▼f2024

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