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Using Live Microorganisms to Modulate Ruminal Fermentation- [electronic resource]
Using Live Microorganisms to Modulate Ruminal Fermentation - [electronic resource]
Using Live Microorganisms to Modulate Ruminal Fermentation- [electronic resource]

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자료유형  
 학위논문파일 국외
최종처리일시  
20240214095837
ISBN  
9798379744984
DDC  
636
저자명  
Monteiro, Hugo Fernando.
서명/저자  
Using Live Microorganisms to Modulate Ruminal Fermentation - [electronic resource]
발행사항  
[S.l.]: : University of Florida., 2021
발행사항  
Ann Arbor : : ProQuest Dissertations & Theses,, 2021
형태사항  
1 online resource(177 p.)
주기사항  
Source: Dissertations Abstracts International, Volume: 84-12, Section: B.
주기사항  
Advisor: Faciola, Antonio.
학위논문주기  
Thesis (Ph.D.)--University of Florida, 2021.
사용제한주기  
This item must not be sold to any third party vendors.
초록/해제  
요약Live microorganisms have been used as feed additives to cattle in order to strategically change the ruminal microbial community dynamics, thus, improving ruminal fermentation and animal performance. The objective of this dissertation was to evaluate different situations that live microorganisms could be used in order to modulate ruminal fermentation. The focus of this work was on ruminal fermentation; therefore, different experimental models were used to understand the mechanism of action of the microorganisms used across studies.In chapter 2, the goal was to evaluate how silage treated with lactic acid bacteria (LAB; Lactobacillus plantarum MTD/1) inoculants could improve ruminal fermentation and milk production in high-producing dairy cows. Dry matter intake, nutrient digestibility, and milk production increased in cows receiveing the treated silage, despite of minor changes in energy-corrected milk (ECM). The increase in ruminal nutrient digestibility were not followed by improvements in ruminal N metabolism, which may explain the lack of responses in ECM.In chapters 3 and 4, the LAB L. plantarum GB-LP1 was tested as direct fed microbials (DFM) on ruminal fermentation of high-producing dairy cow diets. In a series of in vitro studies, the inclusion rate in the diet and the mode of action of the LAB of focus was investigated in comparison with a mix of LAB and lactic acid utilizing bacteria (MLP). All DFM tested in the study improved ruminal N metabolism by negatively affecting some fibrolytic bacterial genera that may dispose proteolytic activity; changes were mostly associated to the fluid phase.In chapter 5, the focus of the study was to create an acute ruminal acidosis scenario in vitro by using a dual-flow continuous culture system, and to feed a mix of lactic acid utilizing bacteria and yeast as DFM in order to ameliorate acidosis. Yeast was tested in this study in combinations with two new strains of Megasphaera eldesnii isolated from the rumen of acidotically challenged beef cattle. Acute ruminal acidosis was successfully induced in vitro; however, the DFM in the study were not able to reduce the acid load or improve ruminal fermentation. Therefore, in chapter 6, a literature review about ruminal acidosis is presented in order to investigate potential factors besides the acid load in the rumen that could explain the ruminal problems seen from this digestive disorder.
일반주제명  
Animal sciences.
일반주제명  
Microbiology.
일반주제명  
Agriculture.
키워드  
Beef-cattle
키워드  
Dairy-cow
키워드  
Lactobacillus-plantarum
키워드  
Megasphaera-elsdenii
키워드  
Rumen
키워드  
Saccharomyces-cerevisiae
기타저자  
University of Florida Animal Sciences
기본자료저록  
Dissertations Abstracts International. 84-12B.
기본자료저록  
Dissertation Abstract International
전자적 위치 및 접속  
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MARC

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■1001  ▼aMonteiro,  Hugo  Fernando.
■24510▼aUsing  Live  Microorganisms  to  Modulate  Ruminal  Fermentation▼h[electronic  resource]
■260    ▼a[S.l.]:▼bUniversity  of  Florida.  ▼c2021
■260  1▼aAnn  Arbor  :▼bProQuest  Dissertations  &  Theses,  ▼c2021
■300    ▼a1  online  resource(177  p.)
■500    ▼aSource:  Dissertations  Abstracts  International,  Volume:  84-12,  Section:  B.
■500    ▼aAdvisor:  Faciola,  Antonio.
■5021  ▼aThesis  (Ph.D.)--University  of  Florida,  2021.
■506    ▼aThis  item  must  not  be  sold  to  any  third  party  vendors.
■520    ▼aLive  microorganisms  have  been  used  as  feed  additives  to  cattle  in  order  to  strategically  change  the  ruminal  microbial  community  dynamics,  thus,  improving  ruminal  fermentation  and  animal  performance.  The  objective  of  this  dissertation  was  to  evaluate  different  situations  that  live  microorganisms  could  be  used  in  order  to  modulate  ruminal  fermentation.  The  focus  of  this  work  was  on  ruminal  fermentation;  therefore,  different  experimental  models  were  used  to  understand  the  mechanism  of  action  of  the  microorganisms  used  across  studies.In  chapter  2,  the  goal  was  to  evaluate  how  silage  treated  with  lactic  acid  bacteria  (LAB;  Lactobacillus  plantarum  MTD/1)  inoculants  could  improve  ruminal  fermentation  and  milk  production  in  high-producing  dairy  cows.  Dry  matter  intake,  nutrient  digestibility,  and  milk  production  increased  in  cows  receiveing  the  treated  silage,  despite  of  minor  changes  in  energy-corrected  milk  (ECM).  The  increase  in  ruminal  nutrient  digestibility  were  not  followed  by  improvements  in  ruminal  N  metabolism,  which  may  explain  the  lack  of  responses  in  ECM.In  chapters  3  and  4,  the  LAB  L.  plantarum  GB-LP1  was  tested  as  direct  fed  microbials  (DFM)  on  ruminal  fermentation  of  high-producing  dairy  cow  diets.  In  a  series  of  in  vitro  studies,  the  inclusion  rate  in  the  diet  and  the  mode  of  action  of  the  LAB  of  focus  was  investigated  in  comparison  with  a  mix  of  LAB  and  lactic  acid  utilizing  bacteria  (MLP).  All  DFM  tested  in  the  study  improved  ruminal  N  metabolism  by  negatively  affecting  some  fibrolytic  bacterial  genera  that  may  dispose  proteolytic  activity;  changes  were  mostly  associated  to  the  fluid  phase.In  chapter  5,  the  focus  of  the  study  was  to  create  an  acute  ruminal  acidosis  scenario  in  vitro  by  using  a  dual-flow  continuous  culture  system,  and  to  feed  a  mix  of  lactic  acid  utilizing  bacteria  and  yeast  as  DFM  in  order  to  ameliorate  acidosis.  Yeast  was  tested  in  this  study  in  combinations  with  two  new  strains  of  Megasphaera  eldesnii  isolated  from  the  rumen  of  acidotically  challenged  beef  cattle.  Acute  ruminal  acidosis  was  successfully  induced  in  vitro;  however,  the  DFM  in  the  study  were  not  able  to  reduce  the  acid  load  or  improve  ruminal  fermentation.  Therefore,  in  chapter  6,  a  literature  review  about  ruminal  acidosis  is  presented  in  order  to  investigate  potential  factors  besides  the  acid  load  in  the  rumen  that  could  explain  the  ruminal  problems  seen  from  this  digestive  disorder.
■590    ▼aSchool  code:  0070.
■650  4▼aAnimal  sciences.
■650  4▼aMicrobiology.
■650  4▼aAgriculture.
■653    ▼aBeef-cattle
■653    ▼aDairy-cow
■653    ▼aLactobacillus-plantarum
■653    ▼aMegasphaera-elsdenii
■653    ▼aRumen
■653    ▼aSaccharomyces-cerevisiae
■690    ▼a0475
■690    ▼a0410
■690    ▼a0473
■71020▼aUniversity  of  Florida▼bAnimal  Sciences.
■7730  ▼tDissertations  Abstracts  International▼g84-12B.
■773    ▼tDissertation  Abstract  International
■790    ▼a0070
■791    ▼aPh.D.
■792    ▼a2021
■793    ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T16930933▼nKERIS▼z이  자료의  원문은  한국교육학술정보원에서  제공합니다.
■980    ▼a202402▼f2024

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