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Discovery and Characterization of Chaperone-Independent Substrates of the CHIP Ubiquitin Ligase- [electronic resource]
Discovery and Characterization of Chaperone-Independent Substrates of the CHIP Ubiquitin Ligase- [electronic resource]
상세정보
- 자료유형
- 학위논문파일 국외
- 최종처리일시
- 20240214101702
- ISBN
- 9798380334655
- DDC
- 574
- 서명/저자
- Discovery and Characterization of Chaperone-Independent Substrates of the CHIP Ubiquitin Ligase - [electronic resource]
- 발행사항
- [S.l.]: : University of California, San Francisco., 2023
- 발행사항
- Ann Arbor : : ProQuest Dissertations & Theses,, 2023
- 형태사항
- 1 online resource(91 p.)
- 주기사항
- Source: Dissertations Abstracts International, Volume: 85-03, Section: B.
- 주기사항
- Includes supplementary digital materials.
- 주기사항
- Advisor: Gestwicki, Jason.
- 학위논문주기
- Thesis (Ph.D.)--University of California, San Francisco, 2023.
- 사용제한주기
- This item must not be sold to any third party vendors.
- 초록/해제
- 요약Protein turnover through endolysosomal degradation and the ubiquitin proteasome system are critical for maintaining protein homeostasis. These pathways protect the cell from accumulation of misfolded proteins, coordinate critical signaling processes, and facilitate recycling pathways that are central to cellular health. An integral member of these systems is the E3 ubiquitin ligase CHIP, which facilitates the turnover of damaged or terminally misfolded proteins. Canonically, substrate recognition by CHIP is dependent on Hsp70 or Hsp90 chaperones, which serve as intermediaries between a misfolded client and CHIP. However, emerging evidence suggests that CHIP also has the capacity to recognize substrates independent of a chaperone binding partner. In this dissertation, we explore the biological relevance of such chaperone-independent substrates by exploring interactions predicted by a biophysical scoring function we previously developed, termed CHIPscore.In the first chapter, I and others describe one such interaction between CHIP and the relatively uncharacterized, membrane-anchored protein CHIC2. We find that CHIC2 binding strongly attenuates CHIP activity, and that CHIC2 knockout phenocopies CHIP knockout in certain cell types, implying that chaperone-independent interactions can sometimes predominate CHIP's biological functions. Furthermore, loss of the CHIP-CHIC2 interaction induces neurodegeneration and shortens lifespan in C. elegans, demonstrating that formation of this chaperone-independent complex is important in animals. We propose that CHIC2 attenuates CHIP activity at the membrane, offering a novel mechanism by which this ubiquitin ligase can be regulated.In the second chapter, I explore additional chaperone-independent interactors beyond CHIC2 that are predicted by CHIPscore. In preliminary results, I demonstrate interactions between CHIP and three additional proteins. These proteins are completely uncharacterized and reside at different subcellular localizations, suggesting that CHIP may be regulated at various locations within the cell through mechanisms that are not yet understood. This work opens substantial new avenues upon which future studies should be based.
- 일반주제명
- Biochemistry.
- 일반주제명
- Chemistry.
- 일반주제명
- Cellular biology.
- 일반주제명
- Neurosciences.
- 키워드
- Chaperone
- 키워드
- Proteomics
- 기타저자
- University of California, San Francisco Chemistry and Chemical Biology
- 기본자료저록
- Dissertations Abstracts International. 85-03B.
- 기본자료저록
- Dissertation Abstract International
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■00520240214101702
■006m o d
■007cr#unu||||||||
■020 ▼a9798380334655
■035 ▼a(MiAaPQ)AAI30635849
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574
■1001 ▼aCallahan, Matthew.▼0(orcid)0000-0002-1854-4212
■24510▼aDiscovery and Characterization of Chaperone-Independent Substrates of the CHIP Ubiquitin Ligase▼h[electronic resource]
■260 ▼a[S.l.]:▼bUniversity of California, San Francisco. ▼c2023
■260 1▼aAnn Arbor :▼bProQuest Dissertations & Theses, ▼c2023
■300 ▼a1 online resource(91 p.)
■500 ▼aSource: Dissertations Abstracts International, Volume: 85-03, Section: B.
■500 ▼aIncludes supplementary digital materials.
■500 ▼aAdvisor: Gestwicki, Jason.
■5021 ▼aThesis (Ph.D.)--University of California, San Francisco, 2023.
■506 ▼aThis item must not be sold to any third party vendors.
■520 ▼aProtein turnover through endolysosomal degradation and the ubiquitin proteasome system are critical for maintaining protein homeostasis. These pathways protect the cell from accumulation of misfolded proteins, coordinate critical signaling processes, and facilitate recycling pathways that are central to cellular health. An integral member of these systems is the E3 ubiquitin ligase CHIP, which facilitates the turnover of damaged or terminally misfolded proteins. Canonically, substrate recognition by CHIP is dependent on Hsp70 or Hsp90 chaperones, which serve as intermediaries between a misfolded client and CHIP. However, emerging evidence suggests that CHIP also has the capacity to recognize substrates independent of a chaperone binding partner. In this dissertation, we explore the biological relevance of such chaperone-independent substrates by exploring interactions predicted by a biophysical scoring function we previously developed, termed CHIPscore.In the first chapter, I and others describe one such interaction between CHIP and the relatively uncharacterized, membrane-anchored protein CHIC2. We find that CHIC2 binding strongly attenuates CHIP activity, and that CHIC2 knockout phenocopies CHIP knockout in certain cell types, implying that chaperone-independent interactions can sometimes predominate CHIP's biological functions. Furthermore, loss of the CHIP-CHIC2 interaction induces neurodegeneration and shortens lifespan in C. elegans, demonstrating that formation of this chaperone-independent complex is important in animals. We propose that CHIC2 attenuates CHIP activity at the membrane, offering a novel mechanism by which this ubiquitin ligase can be regulated.In the second chapter, I explore additional chaperone-independent interactors beyond CHIC2 that are predicted by CHIPscore. In preliminary results, I demonstrate interactions between CHIP and three additional proteins. These proteins are completely uncharacterized and reside at different subcellular localizations, suggesting that CHIP may be regulated at various locations within the cell through mechanisms that are not yet understood. This work opens substantial new avenues upon which future studies should be based.
■590 ▼aSchool code: 0034.
■650 4▼aBiochemistry.
■650 4▼aChemistry.
■650 4▼aCellular biology.
■650 4▼aNeurosciences.
■653 ▼aChaperone
■653 ▼aUbiquitin proteasome system
■653 ▼aProteomics
■653 ▼aMisfolded proteins
■653 ▼aNeurodegeneration
■690 ▼a0487
■690 ▼a0485
■690 ▼a0379
■690 ▼a0317
■71020▼aUniversity of California, San Francisco▼bChemistry and Chemical Biology.
■7730 ▼tDissertations Abstracts International▼g85-03B.
■773 ▼tDissertation Abstract International
■790 ▼a0034
■791 ▼aPh.D.
■792 ▼a2023
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T16934851▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
■980 ▼a202402▼f2024
