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Analysis of Aldoxime-Mediated Metabolic Network in Brassicales and Monocots- [electronic resource]
Analysis of Aldoxime-Mediated Metabolic Network in Brassicales and Monocots- [electronic resource]
상세정보
- 자료유형
- 학위논문파일 국외
- 최종처리일시
- 20240214095917
- ISBN
- 9798379754860
- DDC
- 580
- 서명/저자
- Analysis of Aldoxime-Mediated Metabolic Network in Brassicales and Monocots - [electronic resource]
- 발행사항
- [S.l.]: : University of Florida., 2022
- 발행사항
- Ann Arbor : : ProQuest Dissertations & Theses,, 2022
- 형태사항
- 1 online resource(164 p.)
- 주기사항
- Source: Dissertations Abstracts International, Volume: 84-12, Section: B.
- 주기사항
- Advisor: Kim, Jeongim.
- 학위논문주기
- Thesis (Ph.D.)--University of Florida, 2022.
- 사용제한주기
- This item must not be sold to any third party vendors.
- 초록/해제
- 요약Aldoximes are amino acid derivatives that have been detected throughout the plant kingdom. Aldoximes can serve as precursors of defense compounds such as glucosinolates and cyanogenic glycosides, and recent findings have identified the tryptophan-derived indole-3-acetaldoxime (IAOx) as both a precursor of the growth hormone indole-3-acetic acid (IAA) and a modulator of phenylpropanoid production in the Brassicales species Arabidopsis thaliana and Camelina sativa. Despite these discoveries, many aspects of aldoxime metabolism remain unknown, including the components and distribution of aldoxime-derived auxin biosynthesis, the regulation and biological function of aldoxime-derived auxin and role played by other aldoximes, particularly phenylalanine-derived aldoxime in modulating phenylpropanoid biosynthesis and phenylalanine-derived auxin biosynthesis. The objective of this dissertation was to analyze the growth-related aspects of the aldoxime-mediated metabolic network (auxin biosynthesis and phenylpropanoid repression) in both A. thaliana and monocots, with a specific focus on analyzing the contributions of phenylalanine- and methionine-derived aldoximes. To accomplish this goal, aldoxime feeding assays were used to identify species and mutants capable of aldoxime-derived auxin biosynthesis. Genetic studies in A. thaliana were used to generate mutants lacking or accumulating specific aldoximes to determine their impact on plant growth and phenylpropanoid accumulation, while in maize (Zea mays) and sorghum (Sorghum bicolor) aldoxime metabolism was analyzed in vivo via feeding assays. These experiments resulted in the identification of the phenylalanine-derived aldoxime phenylacetaldoxime (PAOx) as a precursor of the auxin phenylacetic acid (PAA) and the identification of maize and sorghum as species capable of aldoxime-derived auxin biosynthesis. In A. thaliana, PAOx and methionine-derived aldoximes were shown to repress phenylpropanoid biosynthesis in an additive manner and utilizing the same mechanism as IAOx. These findings identify aldoximes as hub metabolites for the regulation of plant growth and defense and lay the framework for further elucidation of this network and its distribution as well as manipulation of this network for improved crop growth under stress conditions.
- 일반주제명
- Plant sciences.
- 일반주제명
- Biology.
- 키워드
- Aldoxime
- 키워드
- Auxin
- 키워드
- Crosstalk
- 키워드
- Phenylpropanoid
- 기타저자
- University of Florida Plant Molecular and Cellular Biology
- 기본자료저록
- Dissertations Abstracts International. 84-12B.
- 기본자료저록
- Dissertation Abstract International
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■00520240214095917
■006m o d
■007cr#unu||||||||
■020 ▼a9798379754860
■035 ▼a(MiAaPQ)AAI29061854
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a580
■1001 ▼aPerez, Veronica Crista.
■24510▼aAnalysis of Aldoxime-Mediated Metabolic Network in Brassicales and Monocots▼h[electronic resource]
■260 ▼a[S.l.]:▼bUniversity of Florida. ▼c2022
■260 1▼aAnn Arbor :▼bProQuest Dissertations & Theses, ▼c2022
■300 ▼a1 online resource(164 p.)
■500 ▼aSource: Dissertations Abstracts International, Volume: 84-12, Section: B.
■500 ▼aAdvisor: Kim, Jeongim.
■5021 ▼aThesis (Ph.D.)--University of Florida, 2022.
■506 ▼aThis item must not be sold to any third party vendors.
■520 ▼aAldoximes are amino acid derivatives that have been detected throughout the plant kingdom. Aldoximes can serve as precursors of defense compounds such as glucosinolates and cyanogenic glycosides, and recent findings have identified the tryptophan-derived indole-3-acetaldoxime (IAOx) as both a precursor of the growth hormone indole-3-acetic acid (IAA) and a modulator of phenylpropanoid production in the Brassicales species Arabidopsis thaliana and Camelina sativa. Despite these discoveries, many aspects of aldoxime metabolism remain unknown, including the components and distribution of aldoxime-derived auxin biosynthesis, the regulation and biological function of aldoxime-derived auxin and role played by other aldoximes, particularly phenylalanine-derived aldoxime in modulating phenylpropanoid biosynthesis and phenylalanine-derived auxin biosynthesis. The objective of this dissertation was to analyze the growth-related aspects of the aldoxime-mediated metabolic network (auxin biosynthesis and phenylpropanoid repression) in both A. thaliana and monocots, with a specific focus on analyzing the contributions of phenylalanine- and methionine-derived aldoximes. To accomplish this goal, aldoxime feeding assays were used to identify species and mutants capable of aldoxime-derived auxin biosynthesis. Genetic studies in A. thaliana were used to generate mutants lacking or accumulating specific aldoximes to determine their impact on plant growth and phenylpropanoid accumulation, while in maize (Zea mays) and sorghum (Sorghum bicolor) aldoxime metabolism was analyzed in vivo via feeding assays. These experiments resulted in the identification of the phenylalanine-derived aldoxime phenylacetaldoxime (PAOx) as a precursor of the auxin phenylacetic acid (PAA) and the identification of maize and sorghum as species capable of aldoxime-derived auxin biosynthesis. In A. thaliana, PAOx and methionine-derived aldoximes were shown to repress phenylpropanoid biosynthesis in an additive manner and utilizing the same mechanism as IAOx. These findings identify aldoximes as hub metabolites for the regulation of plant growth and defense and lay the framework for further elucidation of this network and its distribution as well as manipulation of this network for improved crop growth under stress conditions.
■590 ▼aSchool code: 0070.
■650 4▼aPlant sciences.
■650 4▼aBiology.
■653 ▼aAldoxime
■653 ▼aAuxin
■653 ▼aCrosstalk
■653 ▼aPhenylpropanoid
■653 ▼aSpecialized metabolism
■690 ▼a0479
■690 ▼a0306
■71020▼aUniversity of Florida▼bPlant Molecular and Cellular Biology.
■7730 ▼tDissertations Abstracts International▼g84-12B.
■773 ▼tDissertation Abstract International
■790 ▼a0070
■791 ▼aPh.D.
■792 ▼a2022
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T16931102▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
■980 ▼a202402▼f2024
