Biomonitoring Exposures to Environmental and Dietary Carcinogens by Targeted and Untargeted Mass Spectrometry- [electronic resource]
Biomonitoring Exposures to Environmental and Dietary Carcinogens by Targeted and Untargeted Mass Spectrometry- [electronic resource]
상세정보
- 자료유형
- 학위논문파일 국외
- 최종처리일시
- 20240214095924
- ISBN
- 9798380123761
- DDC
- 543
- 저자명
- Konorev, Dmitri.
- 서명/저자
- Biomonitoring Exposures to Environmental and Dietary Carcinogens by Targeted and Untargeted Mass Spectrometry - [electronic resource]
- 발행사항
- [S.l.]: : University of Minnesota., 2022
- 발행사항
- Ann Arbor : : ProQuest Dissertations & Theses,, 2022
- 형태사항
- 1 online resource(198 p.)
- 주기사항
- Source: Dissertations Abstracts International, Volume: 85-02, Section: B.
- 주기사항
- Advisor: Turesky, Robert J.;Peterson, Lisa A.
- 학위논문주기
- Thesis (Ph.D.)--University of Minnesota, 2022.
- 사용제한주기
- This item must not be sold to any third party vendors.
- 초록/해제
- 요약Humans are exposed to a wide variety of exogenous chemicals that may be implicated in DNA damage and cancer. Typical sources of carcinogen exposure include the diet, environment, and tobacco smoke. There is an epidemiological link between smoking cancer, as well as cooked and red meat consumption, and cancer. Despite extensive study, the chemicals responsible for carcinogenesis are unconfirmed. Specific and sensitive markers of DNA damage by discrete chemicals are needed confirm existing paradigms of carcinogenesis. Chapter 1 outlines the epidemiology and mechanisms by which chemicals from the diet and tobacco can lead to colorectal cancer and bladder cancer.Tobacco smoking is a well-established cause of bladder cancer, as is occupational exposure to high levels of carcinogenic aromatic amines, such as 4-aminobiphenyl and 2-naphthylamine, which are also present in tobacco smoke at low levels. The levels of these compounds in tobacco smoke may be insufficient to bladder carcinogenesis. Other related compounds, such as alkylaniline derivatives, and structurally-related heterocyclic aromatic amines, are present at much higher levels in tobacco smoke and may be risk factors for bladder cancer. Chapter 2 outlines our methodologies to assay a nonpolar, basic fraction of tobacco smoke condensate, containing aromatic amines and heterocyclic aromatic amines, by liquid chromatography coupled to mass spectrometry (LC/MS). These compounds were measured by targeted and untargeted LC/MS as well as with the use of high field asymmetric waveform ion mobility spectrometry coupled to LC/MS to add an additional dimension of separation and reduce sample complexity.Chapter 3 presents our original research in the development of highly sensitive, validated LC/MS methods to measure DNA adducts and abasic sites from cooked meat and tobacco carcinogens in colorectal tissue. These methods were developed using a rat animal model dosed with carcinogens that form DNA adducts associated with colorectal cancer based on previous epidemiological and mechanistic evidence. In Chapter 4, we applied this methodology to human colorectal DNA samples from colorectal cancer patient biopsy samples. We did not detect DNA adducts of exogenous carcinogens but did detect endogenously-formed DNA and abasic sites in these samples. We then contextualized these results within greater paradigms of colorectal cancer carcinogenesis.
- 일반주제명
- Analytical chemistry.
- 일반주제명
- Chemistry.
- 일반주제명
- Toxicology.
- 일반주제명
- Oncology.
- 키워드
- DNA adducts
- 키워드
- FAIMS
- 키워드
- LC/MS
- 기타저자
- University of Minnesota Medicinal Chemistry
- 기본자료저록
- Dissertations Abstracts International. 85-02B.
- 기본자료저록
- Dissertation Abstract International
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■020 ▼a9798380123761
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■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a543
■1001 ▼aKonorev, Dmitri.
■24510▼aBiomonitoring Exposures to Environmental and Dietary Carcinogens by Targeted and Untargeted Mass Spectrometry▼h[electronic resource]
■260 ▼a[S.l.]:▼bUniversity of Minnesota. ▼c2022
■260 1▼aAnn Arbor :▼bProQuest Dissertations & Theses, ▼c2022
■300 ▼a1 online resource(198 p.)
■500 ▼aSource: Dissertations Abstracts International, Volume: 85-02, Section: B.
■500 ▼aAdvisor: Turesky, Robert J.;Peterson, Lisa A.
■5021 ▼aThesis (Ph.D.)--University of Minnesota, 2022.
■506 ▼aThis item must not be sold to any third party vendors.
■520 ▼aHumans are exposed to a wide variety of exogenous chemicals that may be implicated in DNA damage and cancer. Typical sources of carcinogen exposure include the diet, environment, and tobacco smoke. There is an epidemiological link between smoking cancer, as well as cooked and red meat consumption, and cancer. Despite extensive study, the chemicals responsible for carcinogenesis are unconfirmed. Specific and sensitive markers of DNA damage by discrete chemicals are needed confirm existing paradigms of carcinogenesis. Chapter 1 outlines the epidemiology and mechanisms by which chemicals from the diet and tobacco can lead to colorectal cancer and bladder cancer.Tobacco smoking is a well-established cause of bladder cancer, as is occupational exposure to high levels of carcinogenic aromatic amines, such as 4-aminobiphenyl and 2-naphthylamine, which are also present in tobacco smoke at low levels. The levels of these compounds in tobacco smoke may be insufficient to bladder carcinogenesis. Other related compounds, such as alkylaniline derivatives, and structurally-related heterocyclic aromatic amines, are present at much higher levels in tobacco smoke and may be risk factors for bladder cancer. Chapter 2 outlines our methodologies to assay a nonpolar, basic fraction of tobacco smoke condensate, containing aromatic amines and heterocyclic aromatic amines, by liquid chromatography coupled to mass spectrometry (LC/MS). These compounds were measured by targeted and untargeted LC/MS as well as with the use of high field asymmetric waveform ion mobility spectrometry coupled to LC/MS to add an additional dimension of separation and reduce sample complexity.Chapter 3 presents our original research in the development of highly sensitive, validated LC/MS methods to measure DNA adducts and abasic sites from cooked meat and tobacco carcinogens in colorectal tissue. These methods were developed using a rat animal model dosed with carcinogens that form DNA adducts associated with colorectal cancer based on previous epidemiological and mechanistic evidence. In Chapter 4, we applied this methodology to human colorectal DNA samples from colorectal cancer patient biopsy samples. We did not detect DNA adducts of exogenous carcinogens but did detect endogenously-formed DNA and abasic sites in these samples. We then contextualized these results within greater paradigms of colorectal cancer carcinogenesis.
■590 ▼aSchool code: 0130.
■650 4▼aAnalytical chemistry.
■650 4▼aChemistry.
■650 4▼aToxicology.
■650 4▼aOncology.
■653 ▼aDNA adducts
■653 ▼aFAIMS
■653 ▼aLC/MS
■653 ▼aMass spectrometry
■653 ▼aTobacco carcinogens
■690 ▼a0486
■690 ▼a0485
■690 ▼a0383
■690 ▼a0992
■71020▼aUniversity of Minnesota▼bMedicinal Chemistry.
■7730 ▼tDissertations Abstracts International▼g85-02B.
■773 ▼tDissertation Abstract International
■790 ▼a0130
■791 ▼aPh.D.
■792 ▼a2022
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T16931134▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
■980 ▼a202402▼f2024
