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Soil Microbiome of the Guayule (Parthenium argentatum) Root Zone in Diverse Semi-Arid Agricultural Fields- [electronic resource]
Soil Microbiome of the Guayule (Parthenium argentatum) Root Zone in Diverse Semi-Arid Agricultural Fields- [electronic resource]
Detailed Information
- 자료유형
- 학위논문파일 국외
- 최종처리일시
- 20240214101921
- ISBN
- 9798380593441
- DDC
- 630
- 저자명
- Brown, Kyle S.
- 서명/저자
- Soil Microbiome of the Guayule (Parthenium argentatum) Root Zone in Diverse Semi-Arid Agricultural Fields - [electronic resource]
- 발행사항
- [S.l.]: : The University of Arizona., 2023
- 발행사항
- Ann Arbor : : ProQuest Dissertations & Theses,, 2023
- 형태사항
- 1 online resource(123 p.)
- 주기사항
- Source: Dissertations Abstracts International, Volume: 85-04, Section: B.
- 주기사항
- Advisor: Maier, Raina M.;Neilson, Julia W.
- 학위논문주기
- Thesis (Ph.D.)--The University of Arizona, 2023.
- 사용제한주기
- This item must not be sold to any third party vendors.
- 초록/해제
- 요약Guayule (P. argentatum) is an arid-adapted, low water-use crop that is currently being proposed as a replacement for traditional high water-use crops, such as cotton and alfalfa in the southwestern United States (US). Guayule is a prime candidate as an alternative crop in the semi-arid southwest as it requires minimal water and nutrient inputs. Guayule is one of approximately 2000 plants that synthesize rubber, and is one of only two plants to be exploited as a commercial source. Guayule has long been considered as an alternative source of rubber, however extensive work is still required to domesticate and establish guayule as a new or alternative crop in the southwestern US. As an alternative crop, guayule will be grown in a variety of fields with potentially different soil properties and environmental conditions, thus understanding the variability of the soil microbiome is important for guayule growth and production. Previous reports have demonstrated that guayule is highly susceptible to fungal and oomycete soilborne pathogens, with soil moisture and soil texture playing a large role in disease incidence and severity. Species of Fusarium, Macrophomina, Phymatotrichum, Rhizoctonia, Sclerotinia, Sclerotium, and Verticillium have been identified as putative soilborne fungal pathogens of guayule; however, very little information exists on the overall distribution of fungal pathogens in guayule agricultural fields.The purpose of this dissertation was to participate in a two-year irrigation field trial that was part of a large interdisciplinary US Department of Agriculture research effort entitled Sustainable Bioeconomy for Arid Regions. This field trial was conducted at two distinct agricultural fields in southern Arizona with identical experimental designs and similar environmental conditions, but different soil textures to examine: 1) the prevalence and distribution of fungal soilborne pathogens, and 2) the overall soil microbiome. Samples were collected from the guayule root zone in the spring of 2018, and 2019. The internal transcribed spacer (ITS) amplicon sequencing successfully detected four putative guayule fungal soilborne pathogens at both field locations: Fusarium solani, Fusarium oxysporum, Macrophomina phaseolina, and Rhizoctonia solani. The distribution of fungal pathogens across both fields was highly variable, with no significant associations between irrigation treatment and fungal pathogen relative abundance. However, potential disease symptoms were observed on plants in one D150 plot, the highest drip irrigation rate, at the MAC field. F. solani relative abundance in the root zone of symptomatic plants was high; up to 53%, relative to other pathogen relative abundances, suggesting an association with this pathogen and guayule mortality under high irrigation levels in sandy soils where saturated conditions occur. Unlike M. phaseolina, and Rhizoctonia spp, the presence and relative abundance of F. solani did not decrease with plant establishment in either the MAC or Eloy fields. Thus, successful guayule establishment suppresses the relative abundance of some but not all putative fungal pathogens. The data also suggest a significant impact of soil texture on the distribution of the F. oxysporum phylotype.The bacterial/archaeal soil community was analyzed at both field locations to provide a temporal analysis during guayule establishment. Little is known regarding the soil microbiome of guayule and its effect/s on guayule productivity; however, it is well established that microbes enhance plant growth by facilitating nutrient acquisition, suppressing soilborne diseases, and conferring abiotic stress tolerance. The soil microbiome was significantly different at the course phylogenetic level when comparing the two field locations (p 0.001). The soil microbiome was also significantly different when comparing the two sampling years within each location (p 0.001). No significant differences in richness and diversity were observed, with the exception of MAC year 1 samples vs. MAC year 2 samples (p = 0.04). Major differences in the relative abundance of phyla between field locations indicates a strong influence of soil texture on the soil microbiome, and major phylum differences between sampling years indicates guayule establishment does not have a similar effect on the soil microbiome in both fields. Of particular interest was the ammonia-oxidizing archaea Thaumarchaeota, whose relative abundance in both fields was not typical for arid, alkaline soils. This research has demonstrated that two significantly different microbial communities support guayule growth in these two different fields.Taken together, these studies provide the first description and novel insights into guayule root zone soil microbial communities that are potentially relevant to guayule growth and productivity.
- 일반주제명
- Agriculture.
- 일반주제명
- Microbiology.
- 일반주제명
- Soil sciences.
- 키워드
- Fungal pathogens
- 키워드
- Guayule
- 기타저자
- The University of Arizona Environmental Science
- 기본자료저록
- Dissertations Abstracts International. 85-04B.
- 기본자료저록
- Dissertation Abstract International
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
008240612s2023 us |||||||||||||||c||eng d■001000016935347
■00520240214101921
■006m o d
■007cr#unu||||||||
■020 ▼a9798380593441
■035 ▼a(MiAaPQ)AAI30690525
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a630
■1001 ▼aBrown, Kyle S.
■24510▼aSoil Microbiome of the Guayule (Parthenium argentatum) Root Zone in Diverse Semi-Arid Agricultural Fields▼h[electronic resource]
■260 ▼a[S.l.]:▼bThe University of Arizona. ▼c2023
■260 1▼aAnn Arbor :▼bProQuest Dissertations & Theses, ▼c2023
■300 ▼a1 online resource(123 p.)
■500 ▼aSource: Dissertations Abstracts International, Volume: 85-04, Section: B.
■500 ▼aAdvisor: Maier, Raina M.;Neilson, Julia W.
■5021 ▼aThesis (Ph.D.)--The University of Arizona, 2023.
■506 ▼aThis item must not be sold to any third party vendors.
■520 ▼aGuayule (P. argentatum) is an arid-adapted, low water-use crop that is currently being proposed as a replacement for traditional high water-use crops, such as cotton and alfalfa in the southwestern United States (US). Guayule is a prime candidate as an alternative crop in the semi-arid southwest as it requires minimal water and nutrient inputs. Guayule is one of approximately 2000 plants that synthesize rubber, and is one of only two plants to be exploited as a commercial source. Guayule has long been considered as an alternative source of rubber, however extensive work is still required to domesticate and establish guayule as a new or alternative crop in the southwestern US. As an alternative crop, guayule will be grown in a variety of fields with potentially different soil properties and environmental conditions, thus understanding the variability of the soil microbiome is important for guayule growth and production. Previous reports have demonstrated that guayule is highly susceptible to fungal and oomycete soilborne pathogens, with soil moisture and soil texture playing a large role in disease incidence and severity. Species of Fusarium, Macrophomina, Phymatotrichum, Rhizoctonia, Sclerotinia, Sclerotium, and Verticillium have been identified as putative soilborne fungal pathogens of guayule; however, very little information exists on the overall distribution of fungal pathogens in guayule agricultural fields.The purpose of this dissertation was to participate in a two-year irrigation field trial that was part of a large interdisciplinary US Department of Agriculture research effort entitled Sustainable Bioeconomy for Arid Regions. This field trial was conducted at two distinct agricultural fields in southern Arizona with identical experimental designs and similar environmental conditions, but different soil textures to examine: 1) the prevalence and distribution of fungal soilborne pathogens, and 2) the overall soil microbiome. Samples were collected from the guayule root zone in the spring of 2018, and 2019. The internal transcribed spacer (ITS) amplicon sequencing successfully detected four putative guayule fungal soilborne pathogens at both field locations: Fusarium solani, Fusarium oxysporum, Macrophomina phaseolina, and Rhizoctonia solani. The distribution of fungal pathogens across both fields was highly variable, with no significant associations between irrigation treatment and fungal pathogen relative abundance. However, potential disease symptoms were observed on plants in one D150 plot, the highest drip irrigation rate, at the MAC field. F. solani relative abundance in the root zone of symptomatic plants was high; up to 53%, relative to other pathogen relative abundances, suggesting an association with this pathogen and guayule mortality under high irrigation levels in sandy soils where saturated conditions occur. Unlike M. phaseolina, and Rhizoctonia spp, the presence and relative abundance of F. solani did not decrease with plant establishment in either the MAC or Eloy fields. Thus, successful guayule establishment suppresses the relative abundance of some but not all putative fungal pathogens. The data also suggest a significant impact of soil texture on the distribution of the F. oxysporum phylotype.The bacterial/archaeal soil community was analyzed at both field locations to provide a temporal analysis during guayule establishment. Little is known regarding the soil microbiome of guayule and its effect/s on guayule productivity; however, it is well established that microbes enhance plant growth by facilitating nutrient acquisition, suppressing soilborne diseases, and conferring abiotic stress tolerance. The soil microbiome was significantly different at the course phylogenetic level when comparing the two field locations (p 0.001). The soil microbiome was also significantly different when comparing the two sampling years within each location (p 0.001). No significant differences in richness and diversity were observed, with the exception of MAC year 1 samples vs. MAC year 2 samples (p = 0.04). Major differences in the relative abundance of phyla between field locations indicates a strong influence of soil texture on the soil microbiome, and major phylum differences between sampling years indicates guayule establishment does not have a similar effect on the soil microbiome in both fields. Of particular interest was the ammonia-oxidizing archaea Thaumarchaeota, whose relative abundance in both fields was not typical for arid, alkaline soils. This research has demonstrated that two significantly different microbial communities support guayule growth in these two different fields.Taken together, these studies provide the first description and novel insights into guayule root zone soil microbial communities that are potentially relevant to guayule growth and productivity.
■590 ▼aSchool code: 0009.
■650 4▼aAgriculture.
■650 4▼aMicrobiology.
■650 4▼aSoil sciences.
■653 ▼aFungal pathogens
■653 ▼aSoilborne fungal pathogens
■653 ▼aGuayule
■690 ▼a0473
■690 ▼a0410
■690 ▼a0481
■71020▼aThe University of Arizona▼bEnvironmental Science.
■7730 ▼tDissertations Abstracts International▼g85-04B.
■773 ▼tDissertation Abstract International
■790 ▼a0009
■791 ▼aPh.D.
■792 ▼a2023
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T16935347▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
■980 ▼a202402▼f2024
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