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Computational Design of De Novo Protein Nanoparticles for RNA Delivery- [electronic resource]
Computational Design of De Novo Protein Nanoparticles for RNA Delivery- [electronic resource]
상세정보
- 자료유형
- 학위논문파일 국외
- 최종처리일시
- 20240214100058
- ISBN
- 9798379906122
- DDC
- 610
- 서명/저자
- Computational Design of De Novo Protein Nanoparticles for RNA Delivery - [electronic resource]
- 발행사항
- [S.l.]: : University of Washington., 2023
- 발행사항
- Ann Arbor : : ProQuest Dissertations & Theses,, 2023
- 형태사항
- 1 online resource(60 p.)
- 주기사항
- Source: Dissertations Abstracts International, Volume: 85-01, Section: B.
- 주기사항
- Advisor: Baker, David.
- 학위논문주기
- Thesis (Ph.D.)--University of Washington, 2023.
- 사용제한주기
- This item must not be sold to any third party vendors.
- 초록/해제
- 요약Several non-porous RNA encapsulating protein nanoparticles have been reported, but none have been shown to deliver nucleic acids to mammalian cells. Here, I will discuss my efforts to optimize the topology of a de novo pH-responsive trimer for docking into larger protein nanoparticles, and the design and screening of a library of such nanoparticles using a peptide barcoding method. I will also discuss my work engineering a previously reported RNA-encapsulating nanoparticle for RNA delivery in immortalized cell lines. Finally I will discuss my work combining knowledge from all of these efforts into the design of a novel non-porous protein nanoparticle that may provide an optimal scaffold for future efforts in nucleic acid delivery using protein nanoparticles.
- 일반주제명
- Bioengineering.
- 일반주제명
- Biomedical engineering.
- 일반주제명
- Nanoscience.
- 키워드
- Biochemistry
- 키워드
- Delivery
- 키워드
- Protein design
- 키워드
- Nanoparticle
- 기타저자
- University of Washington Bioengineering
- 기본자료저록
- Dissertations Abstracts International. 85-01B.
- 기본자료저록
- Dissertation Abstract International
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■00520240214100058
■006m o d
■007cr#unu||||||||
■020 ▼a9798379906122
■035 ▼a(MiAaPQ)AAI30417284
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a610
■1001 ▼aRichardson, Christian.
■24510▼aComputational Design of De Novo Protein Nanoparticles for RNA Delivery▼h[electronic resource]
■260 ▼a[S.l.]:▼bUniversity of Washington. ▼c2023
■260 1▼aAnn Arbor :▼bProQuest Dissertations & Theses, ▼c2023
■300 ▼a1 online resource(60 p.)
■500 ▼aSource: Dissertations Abstracts International, Volume: 85-01, Section: B.
■500 ▼aAdvisor: Baker, David.
■5021 ▼aThesis (Ph.D.)--University of Washington, 2023.
■506 ▼aThis item must not be sold to any third party vendors.
■520 ▼aSeveral non-porous RNA encapsulating protein nanoparticles have been reported, but none have been shown to deliver nucleic acids to mammalian cells. Here, I will discuss my efforts to optimize the topology of a de novo pH-responsive trimer for docking into larger protein nanoparticles, and the design and screening of a library of such nanoparticles using a peptide barcoding method. I will also discuss my work engineering a previously reported RNA-encapsulating nanoparticle for RNA delivery in immortalized cell lines. Finally I will discuss my work combining knowledge from all of these efforts into the design of a novel non-porous protein nanoparticle that may provide an optimal scaffold for future efforts in nucleic acid delivery using protein nanoparticles.
■590 ▼aSchool code: 0250.
■650 4▼aBioengineering.
■650 4▼aBiomedical engineering.
■650 4▼aNanoscience.
■653 ▼aBiochemistry
■653 ▼aDelivery
■653 ▼aProtein design
■653 ▼aProtein engineering
■653 ▼aNanoparticle
■690 ▼a0202
■690 ▼a0565
■690 ▼a0541
■71020▼aUniversity of Washington▼bBioengineering.
■7730 ▼tDissertations Abstracts International▼g85-01B.
■773 ▼tDissertation Abstract International
■790 ▼a0250
■791 ▼aPh.D.
■792 ▼a2023
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T16931655▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
■980 ▼a202402▼f2024
