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The Fine Balance of Esophageal Homeostasis: The Role of Unique Cells
The Fine Balance of Esophageal Homeostasis: The Role of Unique Cells
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211151336
- ISBN
- 9798382830667
- DDC
- 574.191
- 서명/저자
- The Fine Balance of Esophageal Homeostasis: The Role of Unique Cells
- 발행사항
- [Sl] : University of Pennsylvania, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 107 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 85-12, Section: B.
- 주기사항
- Advisor: Shaffer, Sydney M.
- 학위논문주기
- Thesis (Ph.D.)--University of Pennsylvania, 2024.
- 초록/해제
- 요약This study explores the intricacies of cellular variability and expression in the context of in vitro models of esophageal epithelial cells (EPC2-hTERT) and Barrett's Esophagus (BE), shedding light on the diverse growth dynamics and cellular states within these systems. Esophageal epithelial cells highlighted remarkable growth variability, with approximately 10% of clones exhibiting exponential growth, while the majority displayed logistic growth patterns or halted growth due to non-proliferative cells. Through clonal tracing, mathematical modeling, and cellular barcoding, we elucidated the heritability of growth tendencies and the impact of environmental conditions, such as plating density, on proliferative capabilities. Combining barcoding with single-cell RNA-sequencing (scRNA-seq), we identified the cellular states associated with the highly proliferative clones, which include genes in the WNT and PI3K pathways. Finally, we identified an enrichment of cells resembling the highly proliferative cell state in the proliferating healthy human esophageal epithelium. In BE, advanced spatial transcriptomics revealed a detailed cellular architecture along glandular structures, pinpointing various cell types including previously unidentified ones like tuft and ciliated cells. Notably, ciliated cells at the transitional zone between squamous and columnar epithelium suggest a potential role in the early stages of BE development.
- 일반주제명
- Biophysics
- 일반주제명
- Molecular biology
- 일반주제명
- Cellular biology
- 일반주제명
- Biochemistry
- 키워드
- Epithelial cells
- 키워드
- RNA sequencing
- 키워드
- Logistic growth
- 기타저자
- University of Pennsylvania Biochemistry and Molecular Biophysics
- 기본자료저록
- Dissertations Abstracts International. 85-12B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■020 ▼a9798382830667
■035 ▼a(MiAaPQ)AAI31241909
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574.191
■1001 ▼aReyes Hueros, Raul A.
■24510▼aThe Fine Balance of Esophageal Homeostasis: The Role of Unique Cells
■260 ▼a[Sl]▼bUniversity of Pennsylvania▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a107 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 85-12, Section: B.
■500 ▼aAdvisor: Shaffer, Sydney M.
■5021 ▼aThesis (Ph.D.)--University of Pennsylvania, 2024.
■520 ▼aThis study explores the intricacies of cellular variability and expression in the context of in vitro models of esophageal epithelial cells (EPC2-hTERT) and Barrett's Esophagus (BE), shedding light on the diverse growth dynamics and cellular states within these systems. Esophageal epithelial cells highlighted remarkable growth variability, with approximately 10% of clones exhibiting exponential growth, while the majority displayed logistic growth patterns or halted growth due to non-proliferative cells. Through clonal tracing, mathematical modeling, and cellular barcoding, we elucidated the heritability of growth tendencies and the impact of environmental conditions, such as plating density, on proliferative capabilities. Combining barcoding with single-cell RNA-sequencing (scRNA-seq), we identified the cellular states associated with the highly proliferative clones, which include genes in the WNT and PI3K pathways. Finally, we identified an enrichment of cells resembling the highly proliferative cell state in the proliferating healthy human esophageal epithelium. In BE, advanced spatial transcriptomics revealed a detailed cellular architecture along glandular structures, pinpointing various cell types including previously unidentified ones like tuft and ciliated cells. Notably, ciliated cells at the transitional zone between squamous and columnar epithelium suggest a potential role in the early stages of BE development.
■590 ▼aSchool code: 0175.
■650 4▼aBiophysics
■650 4▼aMolecular biology
■650 4▼aCellular biology
■650 4▼aBiochemistry
■653 ▼aEpithelial cells
■653 ▼aRNA sequencing
■653 ▼aLogistic growth
■653 ▼aGlandular structures
■690 ▼a0786
■690 ▼a0307
■690 ▼a0379
■690 ▼a0487
■71020▼aUniversity of Pennsylvania▼bBiochemistry and Molecular Biophysics.
■7730 ▼tDissertations Abstracts International▼g85-12B.
■790 ▼a0175
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17161298▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
