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The Impact of Acetylcholine Receptors on Osteocytes and Bone Mechanoadaptation
The Impact of Acetylcholine Receptors on Osteocytes and Bone Mechanoadaptation
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211151049
- ISBN
- 9798382840130
- DDC
- 620.8
- 서명/저자
- The Impact of Acetylcholine Receptors on Osteocytes and Bone Mechanoadaptation
- 발행사항
- [Sl] : Cornell University, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 142 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 85-12, Section: B.
- 주기사항
- Advisor: Lewis, Karl.
- 학위논문주기
- Thesis (Ph.D.)--Cornell University, 2024.
- 초록/해제
- 요약Bone's ability to adapt to mechanical loads is attributed to the osteocyte. Osteocytes reside embedded in the calcified bone matrix, where they function as the resident tissue mechanotransducer. Calcium (Ca2+) signaling is a key second messenger in osteocyte mechanotransduction, however the details regarding Ca2+ signaling regulation in osteocytes in vivo are not well stratified. The nervous system is known to regulate bone. Osteocytes express components for acetylcholine (ACh) receptors that are known for rapid firing at the neuromuscular junction. Although ACh is known to impact bone mass and fracture risk, the details regarding ACh signaling in bone mechanotransduction remain largely unexplored.This talk identifies osteocytes as a functional target for cholinergic signaling with impacts on bone mechanoadaptation and Ca2+ signaling. We generated osteocyte-targeted conditional knockout mice to remove key components used by ACh receptors. We assessed tissue material make-up, strength, and formations rates to anabolic loading. We also used intravital imaging via two-photon fluorescent microscopy to visualize the Ca2+ dynamics in osteocytes during active mechanical loading of the bone in living mice. Finally, we explore the ability of light-sheet fluorescent microscopy to create high resolution 3D reconstructions of nerve bodies in whole-mount and intact bone specimens. Our results establish a new signaling axis between the brain and bone via the osteocyte with far reaching implications in bone biology which may be vital to treat bone diseases.
- 일반주제명
- Biomechanics
- 일반주제명
- Physiology
- 일반주제명
- Cellular biology
- 일반주제명
- Neurosciences
- 키워드
- Bone
- 키워드
- Imaging
- 키워드
- Osteocytes
- 기타저자
- Cornell University Mechanical Engineering
- 기본자료저록
- Dissertations Abstracts International. 85-12B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■020 ▼a9798382840130
■035 ▼a(MiAaPQ)AAI31141248
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a620.8
■1001 ▼aMora-Antoinette, Macy.▼0(orcid)0009-0001-8412-1891
■24510▼aThe Impact of Acetylcholine Receptors on Osteocytes and Bone Mechanoadaptation
■260 ▼a[Sl]▼bCornell University▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a142 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 85-12, Section: B.
■500 ▼aAdvisor: Lewis, Karl.
■5021 ▼aThesis (Ph.D.)--Cornell University, 2024.
■520 ▼aBone's ability to adapt to mechanical loads is attributed to the osteocyte. Osteocytes reside embedded in the calcified bone matrix, where they function as the resident tissue mechanotransducer. Calcium (Ca2+) signaling is a key second messenger in osteocyte mechanotransduction, however the details regarding Ca2+ signaling regulation in osteocytes in vivo are not well stratified. The nervous system is known to regulate bone. Osteocytes express components for acetylcholine (ACh) receptors that are known for rapid firing at the neuromuscular junction. Although ACh is known to impact bone mass and fracture risk, the details regarding ACh signaling in bone mechanotransduction remain largely unexplored.This talk identifies osteocytes as a functional target for cholinergic signaling with impacts on bone mechanoadaptation and Ca2+ signaling. We generated osteocyte-targeted conditional knockout mice to remove key components used by ACh receptors. We assessed tissue material make-up, strength, and formations rates to anabolic loading. We also used intravital imaging via two-photon fluorescent microscopy to visualize the Ca2+ dynamics in osteocytes during active mechanical loading of the bone in living mice. Finally, we explore the ability of light-sheet fluorescent microscopy to create high resolution 3D reconstructions of nerve bodies in whole-mount and intact bone specimens. Our results establish a new signaling axis between the brain and bone via the osteocyte with far reaching implications in bone biology which may be vital to treat bone diseases.
■590 ▼aSchool code: 0058.
■650 4▼aBiomechanics
■650 4▼aPhysiology
■650 4▼aCellular biology
■650 4▼aNeurosciences
■653 ▼aBone
■653 ▼aCalcium signaling
■653 ▼aImaging
■653 ▼aMechanoadaptation
■653 ▼aNicotinic acetylcholine receptors
■653 ▼aOsteocytes
■690 ▼a0648
■690 ▼a0379
■690 ▼a0317
■690 ▼a0719
■71020▼aCornell University▼bMechanical Engineering.
■7730 ▼tDissertations Abstracts International▼g85-12B.
■790 ▼a0058
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17160617▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
