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Development of CRISPR-Based Tools for the Dissection of Chromatin Organization and Dynamics
Development of CRISPR-Based Tools for the Dissection of Chromatin Organization and Dynamics
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211151939
- ISBN
- 9798384463269
- DDC
- 574.191
- 저자명
- Chang, Yi-Che.
- 서명/저자
- Development of CRISPR-Based Tools for the Dissection of Chromatin Organization and Dynamics
- 발행사항
- [Sl] : Princeton University, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 141 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 86-04, Section: B.
- 주기사항
- Advisor: Brangwynne, Clifford P.
- 학위논문주기
- Thesis (Ph.D.)--Princeton University, 2024.
- 초록/해제
- 요약Eukaryotic cells compartmentalize intracellular components to segregate essential biological functions. While membrane-bound organelles have traditionally served this purpose, liquid-liquid phase separation (LLPS) emerges as a complementary mechanism in cellular spatial organization without membrane-bound structures. This dissertation investigates LLPS' roles in nucleus and genome organization by segregating functionally distinct nuclear compartments, each featuring a distinct set of biochemical reactions. We first discuss the development of CasDrop, a CRISPR/Cas9-based optogenetic tool, and how it can be used to study condensate-genome interactions. Specific focus is given to how transcriptionally active and inactive condensates can exclude each other, along with chromatin compartments enriched in active or inactive proteins. These chemical and mechanical effects can impact the local dynamics of the genome structure and its functional states. We further demonstrate that condensate surface tension can be harnessed to rapidly restructure genome organization. Finally, we reveal the complex, heterogenous organization within euchromatin; and probed its chromatin dynamics to investigate the correlation between transcriptional activity and chromatin dynamics at a genome-wide scale.
- 일반주제명
- Biophysics
- 일반주제명
- Bioengineering
- 일반주제명
- Cellular biology
- 키워드
- Eukaryotic cells
- 키워드
- Genome structure
- 키워드
- Optogenetic tool
- 기타저자
- Princeton University Chemistry
- 기본자료저록
- Dissertations Abstracts International. 86-04B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■020 ▼a9798384463269
■035 ▼a(MiAaPQ)AAI31302040
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574.191
■1001 ▼aChang, Yi-Che.
■24510▼aDevelopment of CRISPR-Based Tools for the Dissection of Chromatin Organization and Dynamics
■260 ▼a[Sl]▼bPrinceton University▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a141 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-04, Section: B.
■500 ▼aAdvisor: Brangwynne, Clifford P.
■5021 ▼aThesis (Ph.D.)--Princeton University, 2024.
■520 ▼aEukaryotic cells compartmentalize intracellular components to segregate essential biological functions. While membrane-bound organelles have traditionally served this purpose, liquid-liquid phase separation (LLPS) emerges as a complementary mechanism in cellular spatial organization without membrane-bound structures. This dissertation investigates LLPS' roles in nucleus and genome organization by segregating functionally distinct nuclear compartments, each featuring a distinct set of biochemical reactions. We first discuss the development of CasDrop, a CRISPR/Cas9-based optogenetic tool, and how it can be used to study condensate-genome interactions. Specific focus is given to how transcriptionally active and inactive condensates can exclude each other, along with chromatin compartments enriched in active or inactive proteins. These chemical and mechanical effects can impact the local dynamics of the genome structure and its functional states. We further demonstrate that condensate surface tension can be harnessed to rapidly restructure genome organization. Finally, we reveal the complex, heterogenous organization within euchromatin; and probed its chromatin dynamics to investigate the correlation between transcriptional activity and chromatin dynamics at a genome-wide scale.
■590 ▼aSchool code: 0181.
■650 4▼aBiophysics
■650 4▼aBioengineering
■650 4▼aCellular biology
■653 ▼aEukaryotic cells
■653 ▼aGenome structure
■653 ▼aHeterogenous organization
■653 ▼aChromatin dynamics
■653 ▼aOptogenetic tool
■690 ▼a0786
■690 ▼a0202
■690 ▼a0379
■71020▼aPrinceton University▼bChemistry.
■7730 ▼tDissertations Abstracts International▼g86-04B.
■790 ▼a0181
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17162160▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
