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Vitamin B12, One-Carbon Metabolism, and Metabolic Health in Women of Reproductive Age
Vitamin B12, One-Carbon Metabolism, and Metabolic Health in Women of Reproductive Age
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211151335
- ISBN
- 9798382843421
- DDC
- 641
- 서명/저자
- Vitamin B12, One-Carbon Metabolism, and Metabolic Health in Women of Reproductive Age
- 발행사항
- [Sl] : Cornell University, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 290 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 85-12, Section: A.
- 주기사항
- Advisor: Mehta, Julia.
- 학위논문주기
- Thesis (Ph.D.)--Cornell University, 2024.
- 초록/해제
- 요약Background: Adequate vitamin B12 and folate status during the periconceptional period is important for preventing anemia and neural tube defects (NTD). However, there is limited population-level data on vitamin B12 and folate biomarkers and health outcomes in women of reproductive of age to inform public health screening and interventions. A population-based biomarker survey was conducted among women in Southern India to inform a randomized efficacy trial of quadruple-fortified salt (QFS; iron, iodine, folic acid, vitamin B12).Methods: Participants (n=980 women 15-40y; not pregnant or lactating) in the biomarker survey were assessed for total vitamin B12 (chemiluminescence), holotranscobalamin (enzyme-immunoassay), methylmalonic acid and homocysteine (GC-MS), red blood cell (RBC) and serum folate (WHO-recommended microbiologic assay), and HbA1c (nephelometry). A random subset (n=250) of archived urine samples were assessed for total arsenic (ICP-MS) and creatinine (enzymatic chemistry analyzer). Anthropometric and blood pressure measurements were collected in triplicate. Bioelectrical impedance analysis was used to evaluate whole body and trunk fat. Linear and binomial regression were used to examine associations of vitamin B12 status with metabolic outcomes and urinary total arsenic with micronutrient biomarkers. A previously developed Bayesian model and RBC folate and arsenic distributions in this population were used to predict NTD prevalence per 10,000 births. The acceptability of QFS was conducted among women 18-49y (n=77) and their households using a household-level randomized crossover trial (weighed salt use, hedonic ratings) and individual-level triangle tests. Mixed models were used to examine hedonic ratings and salt use; salt type, sequence, and period were included as fixed effects, and household was included as a random effect. Binomial tests were used to evaluate sensory discrimination of salt type in triangle tests among women.Results: One-third of women had overweight (body mass index≥25.0 kg/m2), 20.8% had elevated waist-to-hip ratio (≥0.85), one-fourth had elevated HbA1c (≥5.7%), and 18.6% had hypertension. Lower vitamin B12 status was associated with indicators of central adiposity, overweight, and elevated percent body fat. Urinary arsenic concentrations were detectable in all samples; 80% of women had urinary arsenic 10 µg/g while 50 µg/g. Women with urinary arsenic 10 µg/g had significantly lower RBC folate concentrations and higher predicted NTD prevalence, compared to women with concentrations ≤10 µg/g. Mean hedonic ratings and weighed household salt use did not differ by salt type and significantly increased over the 3-week period, indicating a period effect independent of salt type or sequence. In triangle tests, food samples prepared with QFS, DFS, or iodized salt were not distinguishable.Conclusions: Vitamin B12 deficiency was associated with central adiposity and overweight. Arsenic exposure at levels similar to the United States may modify RBC folate concentrations and population-level NTD risk. These data suggest an opportunity for informing public health screening and interventions. Findings informed a randomized trial of QFS in this population.
- 일반주제명
- Nutrition
- 일반주제명
- Womens studies
- 일반주제명
- Biochemistry
- 일반주제명
- Obstetrics
- 키워드
- Folate status
- 키워드
- Southern India
- 키워드
- Vitamin B12
- 키워드
- Urinary arsenic
- 기타저자
- Cornell University Nutrition
- 기본자료저록
- Dissertations Abstracts International. 85-12A.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■00520250211151335
■006m o d
■007cr#unu||||||||
■020 ▼a9798382843421
■035 ▼a(MiAaPQ)AAI31241807
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a641
■1001 ▼aGuetterman, Heather.▼0(orcid)0000-0003-3346-5552
■24510▼aVitamin B12, One-Carbon Metabolism, and Metabolic Health in Women of Reproductive Age
■260 ▼a[Sl]▼bCornell University▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a290 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 85-12, Section: A.
■500 ▼aAdvisor: Mehta, Julia.
■5021 ▼aThesis (Ph.D.)--Cornell University, 2024.
■520 ▼aBackground: Adequate vitamin B12 and folate status during the periconceptional period is important for preventing anemia and neural tube defects (NTD). However, there is limited population-level data on vitamin B12 and folate biomarkers and health outcomes in women of reproductive of age to inform public health screening and interventions. A population-based biomarker survey was conducted among women in Southern India to inform a randomized efficacy trial of quadruple-fortified salt (QFS; iron, iodine, folic acid, vitamin B12).Methods: Participants (n=980 women 15-40y; not pregnant or lactating) in the biomarker survey were assessed for total vitamin B12 (chemiluminescence), holotranscobalamin (enzyme-immunoassay), methylmalonic acid and homocysteine (GC-MS), red blood cell (RBC) and serum folate (WHO-recommended microbiologic assay), and HbA1c (nephelometry). A random subset (n=250) of archived urine samples were assessed for total arsenic (ICP-MS) and creatinine (enzymatic chemistry analyzer). Anthropometric and blood pressure measurements were collected in triplicate. Bioelectrical impedance analysis was used to evaluate whole body and trunk fat. Linear and binomial regression were used to examine associations of vitamin B12 status with metabolic outcomes and urinary total arsenic with micronutrient biomarkers. A previously developed Bayesian model and RBC folate and arsenic distributions in this population were used to predict NTD prevalence per 10,000 births. The acceptability of QFS was conducted among women 18-49y (n=77) and their households using a household-level randomized crossover trial (weighed salt use, hedonic ratings) and individual-level triangle tests. Mixed models were used to examine hedonic ratings and salt use; salt type, sequence, and period were included as fixed effects, and household was included as a random effect. Binomial tests were used to evaluate sensory discrimination of salt type in triangle tests among women.Results: One-third of women had overweight (body mass index≥25.0 kg/m2), 20.8% had elevated waist-to-hip ratio (≥0.85), one-fourth had elevated HbA1c (≥5.7%), and 18.6% had hypertension. Lower vitamin B12 status was associated with indicators of central adiposity, overweight, and elevated percent body fat. Urinary arsenic concentrations were detectable in all samples; 80% of women had urinary arsenic 10 µg/g while 50 µg/g. Women with urinary arsenic 10 µg/g had significantly lower RBC folate concentrations and higher predicted NTD prevalence, compared to women with concentrations ≤10 µg/g. Mean hedonic ratings and weighed household salt use did not differ by salt type and significantly increased over the 3-week period, indicating a period effect independent of salt type or sequence. In triangle tests, food samples prepared with QFS, DFS, or iodized salt were not distinguishable.Conclusions: Vitamin B12 deficiency was associated with central adiposity and overweight. Arsenic exposure at levels similar to the United States may modify RBC folate concentrations and population-level NTD risk. These data suggest an opportunity for informing public health screening and interventions. Findings informed a randomized trial of QFS in this population.
■590 ▼aSchool code: 0058.
■650 4▼aNutrition
■650 4▼aWomens studies
■650 4▼aBiochemistry
■650 4▼aObstetrics
■653 ▼aFolate status
■653 ▼aSouthern India
■653 ▼aPericonceptional period
■653 ▼aVitamin B12
■653 ▼aUrinary arsenic
■690 ▼a0570
■690 ▼a0453
■690 ▼a0487
■690 ▼a0380
■71020▼aCornell University▼bNutrition.
■7730 ▼tDissertations Abstracts International▼g85-12A.
■790 ▼a0058
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17161293▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
