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Deciphering the Molecular Biology of Diaphorina citri and "Candidatus Liberibacter asiaticus" Interactions: Insights Into Huanglongbing and Vector Capacity
Deciphering the Molecular Biology of Diaphorina citri and "Candidatus Liberibacter asiaticus" Interactions: Insights Into Huanglongbing and Vector Capacity
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211151359
- ISBN
- 9798382843469
- DDC
- 581
- 서명/저자
- Deciphering the Molecular Biology of Diaphorina citri and Candidatus Liberibacter asiaticus Interactions: Insights Into Huanglongbing and Vector Capacity
- 발행사항
- [Sl] : Cornell University, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 205 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 85-12, Section: B.
- 주기사항
- Includes supplementary digital materials.
- 주기사항
- Advisor: Cilia, Michelle.
- 학위논문주기
- Thesis (Ph.D.)--Cornell University, 2024.
- 초록/해제
- 요약Huanglongbing (HLB), also known as citrus greening, poses a significant threat to the citrus industry worldwide, with devastating effects on tree health and fruit production. The disease is primarily spread by the Asian citrus psyllid, Diaphorina citri, which is the vector for the bacterium "Candidatus Liberibacter asiaticus" (CLas), the causal agent of HLB. Long term management of HLB hinges on sustainable control of the vector, reducing transmission of CLas to new, healthy trees. Understanding molecular interactions between D. citri and CLas is crucial for developing effective vector intervention methods. Vector competency, influenced by genetics, environment, and pathogen interactions, determines a vector's ability to transmit a pathogen. In D. citri, vector competency is likely regulated by distinct tissue barriers, which CLas must overcome to be successfully acquired and transmitted. However, understanding the direct barrier-CLas interactions that lead to either successful or failed acquisition and transmission has yet to be determined. In this dissertation, we conducted a comparative transcriptome analysis of healthy and CLas-infected D. citri, focusing on multiple organs including bacteriomes, salivary glands, and heads, in order to track CLas-D. citri interactions throughout the vector body. Using the latest D. citri genome assembly (v3.0), we integrated new transcriptome data with previously published datasets to elucidate the impact of CLas on the transcriptional landscape of D. citri organs involved in circulative, propagative transmission. In order to begin bridging the gap between laboratory-generated transcriptomic results and the reality of wild vector population diversity, we sampled four active citrus groves and assessed hundreds of psyllids for their individual vector competency via population infection rate and CLas titer. Our findings revealed population-level differences in D. citri vector competency, likely influenced by a combination of genetic and environmental factors. From these wild psyllid samples, we aimed to determine if vector competency in D. citri is genetically linked. Previous studies have shown that natural variation in vector capacity is heritable. To assess the genomics of D. citri-CLas interactions, whole genome sequencing of 500 individuals to 7x depth allowed us to associate CLas titer with genomic variability via single nucleotide polymorphisms (SNPs) in a GWAS - one of the first for an agricultural insect vector. We have identified dozens of loci across the genome that make promising targets for interdiction of CLas transmission. The variation in vector competency recorded from both lab and field-sourced samples underscores the importance of assessing multi-trophic pathosystems and disease epidemiology from multiple perspectives. Lessons learned through our vector-pathogen molecular-omics analyses further emphasize this importance. Genome sequencing was foundational for exploring molecular interactions between D. citri and CLas. Further development of omics resources for insect vectors of agriculturally important pathogens is essential.
- 일반주제명
- Plant pathology
- 일반주제명
- Entomology
- 일반주제명
- Agriculture
- 일반주제명
- Plant sciences
- 일반주제명
- Molecular biology
- 키워드
- GWAS
- 키워드
- Huanglongbing
- 키워드
- Psyllids
- 키워드
- Transcriptomics
- 키워드
- Vector biology
- 기타저자
- Cornell University Plant Pathology and Plant-Microbe Biology
- 기본자료저록
- Dissertations Abstracts International. 85-12B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■020 ▼a9798382843469
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■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a581
■1001 ▼aMann, Marina Alexandria.▼0(orcid)0000-0003-1924-047X
■24510▼aDeciphering the Molecular Biology of Diaphorina citri and "Candidatus Liberibacter asiaticus" Interactions: Insights Into Huanglongbing and Vector Capacity
■260 ▼a[Sl]▼bCornell University▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a205 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 85-12, Section: B.
■500 ▼aIncludes supplementary digital materials.
■500 ▼aAdvisor: Cilia, Michelle.
■5021 ▼aThesis (Ph.D.)--Cornell University, 2024.
■520 ▼aHuanglongbing (HLB), also known as citrus greening, poses a significant threat to the citrus industry worldwide, with devastating effects on tree health and fruit production. The disease is primarily spread by the Asian citrus psyllid, Diaphorina citri, which is the vector for the bacterium "Candidatus Liberibacter asiaticus" (CLas), the causal agent of HLB. Long term management of HLB hinges on sustainable control of the vector, reducing transmission of CLas to new, healthy trees. Understanding molecular interactions between D. citri and CLas is crucial for developing effective vector intervention methods. Vector competency, influenced by genetics, environment, and pathogen interactions, determines a vector's ability to transmit a pathogen. In D. citri, vector competency is likely regulated by distinct tissue barriers, which CLas must overcome to be successfully acquired and transmitted. However, understanding the direct barrier-CLas interactions that lead to either successful or failed acquisition and transmission has yet to be determined. In this dissertation, we conducted a comparative transcriptome analysis of healthy and CLas-infected D. citri, focusing on multiple organs including bacteriomes, salivary glands, and heads, in order to track CLas-D. citri interactions throughout the vector body. Using the latest D. citri genome assembly (v3.0), we integrated new transcriptome data with previously published datasets to elucidate the impact of CLas on the transcriptional landscape of D. citri organs involved in circulative, propagative transmission. In order to begin bridging the gap between laboratory-generated transcriptomic results and the reality of wild vector population diversity, we sampled four active citrus groves and assessed hundreds of psyllids for their individual vector competency via population infection rate and CLas titer. Our findings revealed population-level differences in D. citri vector competency, likely influenced by a combination of genetic and environmental factors. From these wild psyllid samples, we aimed to determine if vector competency in D. citri is genetically linked. Previous studies have shown that natural variation in vector capacity is heritable. To assess the genomics of D. citri-CLas interactions, whole genome sequencing of 500 individuals to 7x depth allowed us to associate CLas titer with genomic variability via single nucleotide polymorphisms (SNPs) in a GWAS - one of the first for an agricultural insect vector. We have identified dozens of loci across the genome that make promising targets for interdiction of CLas transmission. The variation in vector competency recorded from both lab and field-sourced samples underscores the importance of assessing multi-trophic pathosystems and disease epidemiology from multiple perspectives. Lessons learned through our vector-pathogen molecular-omics analyses further emphasize this importance. Genome sequencing was foundational for exploring molecular interactions between D. citri and CLas. Further development of omics resources for insect vectors of agriculturally important pathogens is essential.
■590 ▼aSchool code: 0058.
■650 4▼aPlant pathology
■650 4▼aEntomology
■650 4▼aAgriculture
■650 4▼aPlant sciences
■650 4▼aMolecular biology
■653 ▼aAsian citrus psyllid
■653 ▼aGWAS
■653 ▼aHuanglongbing
■653 ▼aPsyllids
■653 ▼aTranscriptomics
■653 ▼aVector biology
■690 ▼a0480
■690 ▼a0353
■690 ▼a0473
■690 ▼a0479
■690 ▼a0307
■71020▼aCornell University▼bPlant Pathology and Plant-Microbe Biology.
■7730 ▼tDissertations Abstracts International▼g85-12B.
■790 ▼a0058
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17161461▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
