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Dissection of Cell Plate Dynamics During Cytokinesis and the Role of Callose in Cell Plate Maturation
Dissection of Cell Plate Dynamics During Cytokinesis and the Role of Callose in Cell Plate Maturation
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211151038
- ISBN
- 9798382606286
- DDC
- 574
- 서명/저자
- Dissection of Cell Plate Dynamics During Cytokinesis and the Role of Callose in Cell Plate Maturation
- 발행사항
- [Sl] : University of California, Davis, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 114 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 85-11, Section: B.
- 주기사항
- Advisor: Drakakaki, Georgia.
- 학위논문주기
- Thesis (Ph.D.)--University of California, Davis, 2024.
- 초록/해제
- 요약The cell wall is an integral and dynamic structure surrounding plant cells, involved in plant growth and development. During cytokinesis, cell plate formation takes place, via fusion of cytokinetic vesicles, that after membrane transformation and deposition of polysaccharides transitions to a new cross wall. Callose, a β(1,3) glucan polysaccharide, is transiently deposited at the cell plate during this fundamental process. Callose deposition is thought to play a vital role in stabilizing the cell plate and contributing to a spreading force during cell plate expansion. Cytokinesis-specific callose synthases are recalcitrant to study by genetic means, because null mutations in the corresponding genes cause lethality. Additionally, limitations in live cell imaging of polysaccharides add to the difficulties in elucidating the biological role and regulation of callose biosynthesis at the cell plate. To overcome these challenges, I have taken a multidisciplinary approach. To better understand the transition from a vesicular network to a fenestrated sheet and finally a mature cell plate, we implemented a modeling approach, adopting the Helfrich energy that examines the elastic properties of lipid bilayers. Our model predicts the requirement of a spreading/stabilizing force for cell plate expansion and maturation. The transient accumulation of callose, coinciding with the predicted cell plate stages requiring this spreading force, are consistent with the proposed model. Furthermore, we used a specific cytokinetic callose inhibitor, Endosidin7 (ES7), to dissect the role of callose. ES7 does not inhibit wound or stress-induced callose deposition; however, it specifically causes failure in cell plate expansion and maturation, indicating that callose is a contributor to the stabilizing or spreading force as predicted by our model. We further used advanced light microscopic techniques to explore cell plate dynamics. I studied cell plate development across four dimensions using a cytokinesis-specific GTPase YFP-RABA2a as a vesicle marker. With the aid of a robust cell plate volume analysis pipeline, I identified three easily trackable cell plate developmental phases that can be interrogated to study cell plate development. Inhibition of callose through ES7 suppressed phase transitions, establishing a critical role and timing of polysaccharide deposition in cell plate expansion and maturation. Using this suite of interdisciplinary techniques, we contributed to breaking apart the molecular black box surrounding callose deposition during cytokinesis.
- 일반주제명
- Cellular biology
- 일반주제명
- Biophysics
- 일반주제명
- Molecular biology
- 일반주제명
- Plant sciences
- 일반주제명
- Biology
- 키워드
- Cell plate
- 기타저자
- University of California, Davis Plant Biology
- 기본자료저록
- Dissertations Abstracts International. 85-11B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
008250123s2024 us c eng d■001000017160546
■00520250211151038
■006m o d
■007cr#unu||||||||
■020 ▼a9798382606286
■035 ▼a(MiAaPQ)AAI31139532
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574
■1001 ▼aSinclair, Rosalie Mason.
■24510▼aDissection of Cell Plate Dynamics During Cytokinesis and the Role of Callose in Cell Plate Maturation
■260 ▼a[Sl]▼bUniversity of California, Davis▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a114 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 85-11, Section: B.
■500 ▼aAdvisor: Drakakaki, Georgia.
■5021 ▼aThesis (Ph.D.)--University of California, Davis, 2024.
■520 ▼aThe cell wall is an integral and dynamic structure surrounding plant cells, involved in plant growth and development. During cytokinesis, cell plate formation takes place, via fusion of cytokinetic vesicles, that after membrane transformation and deposition of polysaccharides transitions to a new cross wall. Callose, a β(1,3) glucan polysaccharide, is transiently deposited at the cell plate during this fundamental process. Callose deposition is thought to play a vital role in stabilizing the cell plate and contributing to a spreading force during cell plate expansion. Cytokinesis-specific callose synthases are recalcitrant to study by genetic means, because null mutations in the corresponding genes cause lethality. Additionally, limitations in live cell imaging of polysaccharides add to the difficulties in elucidating the biological role and regulation of callose biosynthesis at the cell plate. To overcome these challenges, I have taken a multidisciplinary approach. To better understand the transition from a vesicular network to a fenestrated sheet and finally a mature cell plate, we implemented a modeling approach, adopting the Helfrich energy that examines the elastic properties of lipid bilayers. Our model predicts the requirement of a spreading/stabilizing force for cell plate expansion and maturation. The transient accumulation of callose, coinciding with the predicted cell plate stages requiring this spreading force, are consistent with the proposed model. Furthermore, we used a specific cytokinetic callose inhibitor, Endosidin7 (ES7), to dissect the role of callose. ES7 does not inhibit wound or stress-induced callose deposition; however, it specifically causes failure in cell plate expansion and maturation, indicating that callose is a contributor to the stabilizing or spreading force as predicted by our model. We further used advanced light microscopic techniques to explore cell plate dynamics. I studied cell plate development across four dimensions using a cytokinesis-specific GTPase YFP-RABA2a as a vesicle marker. With the aid of a robust cell plate volume analysis pipeline, I identified three easily trackable cell plate developmental phases that can be interrogated to study cell plate development. Inhibition of callose through ES7 suppressed phase transitions, establishing a critical role and timing of polysaccharide deposition in cell plate expansion and maturation. Using this suite of interdisciplinary techniques, we contributed to breaking apart the molecular black box surrounding callose deposition during cytokinesis.
■590 ▼aSchool code: 0029.
■650 4▼aCellular biology
■650 4▼aBiophysics
■650 4▼aMolecular biology
■650 4▼aPlant sciences
■650 4▼aBiology
■653 ▼aCallose deposition
■653 ▼aCell plate
■653 ▼aLattice light sheet microscopy
■653 ▼aPlant cell division
■653 ▼aPlant cytokinesis
■653 ▼aQuantitative image analysis
■690 ▼a0379
■690 ▼a0786
■690 ▼a0307
■690 ▼a0306
■690 ▼a0479
■71020▼aUniversity of California, Davis▼bPlant Biology.
■7730 ▼tDissertations Abstracts International▼g85-11B.
■790 ▼a0029
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17160546▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
