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Long-Term Non-Bleaching Nanoscale Imaging by Plasmonic Nanoscope
Long-Term Non-Bleaching Nanoscale Imaging by Plasmonic Nanoscope
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211152055
- ISBN
- 9798382738963
- DDC
- 610
- 저자명
- Zhao, Xintao.
- 서명/저자
- Long-Term Non-Bleaching Nanoscale Imaging by Plasmonic Nanoscope
- 발행사항
- [Sl] : University of Michigan, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 108 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 85-12, Section: B.
- 주기사항
- Advisor: Lee, Somin Eunice.
- 학위논문주기
- Thesis (Ph.D.)--University of Michigan, 2024.
- 초록/해제
- 요약Long-term observation of live cells and activities, including cell dynamics and cellular responses, is a crucial technology in the field of cell studies such as cancer diagnostics and disease therapy. The challenges of long-term imaging fall under four categories: selection of cell type amenable to long-term imaging; precise control of the cellular environment under in-vitro system; imaging system and probe design to overcome photobleaching, phototoxicity, and to achieve high resolution; efficient data extraction and post data analysis process. Here we present a long-term non-bleaching nanoscale imaging method by plasmonic nanoscope. Through plasmonic molecular marker (gold nanorods) labeling and phase intensity separation of nanoprobes, we achieved non-bleaching, nanoscale imaging of biological systems, such as actin networks. While the standard fluorophore-based method limiting the imaging windows to minutes ( 1 hour), using our optimized imaging system, we were able to continuously observe long-term biological dynamics in the window of hours to days as well as achieving sub-10 nm resolution. The innovative nanoscale imaging system utilizing a plasmonic nanoscope has been successfully developed to address challenges encountered in long-term bio-imaging and demonstrated by visualization and quantitative analysis of actin dynamics during the disassembly process by actin binding protein (cofilin). Furthermore, we demonstrated live-cell imaging using nanoscale plasmonic nanoscope which allowed for the exploration of the orientation distribution of extracellular beta-actin network within a cell-division cycle.
- 일반주제명
- Biomedical engineering
- 일반주제명
- Electrical engineering
- 일반주제명
- Computer engineering
- 일반주제명
- Cellular biology
- 일반주제명
- Nanoscience
- 키워드
- Plasmonics
- 키워드
- Nanoscope
- 기타저자
- University of Michigan Electrical and Computer Engineering
- 기본자료저록
- Dissertations Abstracts International. 85-12B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■006m o d
■007cr#unu||||||||
■020 ▼a9798382738963
■035 ▼a(MiAaPQ)AAI31348901
■035 ▼a(MiAaPQ)umichrackham005549
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a610
■1001 ▼aZhao, Xintao.
■24510▼aLong-Term Non-Bleaching Nanoscale Imaging by Plasmonic Nanoscope
■260 ▼a[Sl]▼bUniversity of Michigan▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a108 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 85-12, Section: B.
■500 ▼aAdvisor: Lee, Somin Eunice.
■5021 ▼aThesis (Ph.D.)--University of Michigan, 2024.
■520 ▼aLong-term observation of live cells and activities, including cell dynamics and cellular responses, is a crucial technology in the field of cell studies such as cancer diagnostics and disease therapy. The challenges of long-term imaging fall under four categories: selection of cell type amenable to long-term imaging; precise control of the cellular environment under in-vitro system; imaging system and probe design to overcome photobleaching, phototoxicity, and to achieve high resolution; efficient data extraction and post data analysis process. Here we present a long-term non-bleaching nanoscale imaging method by plasmonic nanoscope. Through plasmonic molecular marker (gold nanorods) labeling and phase intensity separation of nanoprobes, we achieved non-bleaching, nanoscale imaging of biological systems, such as actin networks. While the standard fluorophore-based method limiting the imaging windows to minutes ( 1 hour), using our optimized imaging system, we were able to continuously observe long-term biological dynamics in the window of hours to days as well as achieving sub-10 nm resolution. The innovative nanoscale imaging system utilizing a plasmonic nanoscope has been successfully developed to address challenges encountered in long-term bio-imaging and demonstrated by visualization and quantitative analysis of actin dynamics during the disassembly process by actin binding protein (cofilin). Furthermore, we demonstrated live-cell imaging using nanoscale plasmonic nanoscope which allowed for the exploration of the orientation distribution of extracellular beta-actin network within a cell-division cycle.
■590 ▼aSchool code: 0127.
■650 4▼aBiomedical engineering
■650 4▼aElectrical engineering
■650 4▼aComputer engineering
■650 4▼aCellular biology
■650 4▼aNanoscience
■653 ▼aPlasmonics
■653 ▼aNanoscope
■653 ▼aLong-term bio-imaging
■653 ▼aGold nanoparticle
■653 ▼aCellular responses
■690 ▼a0544
■690 ▼a0541
■690 ▼a0565
■690 ▼a0379
■690 ▼a0464
■71020▼aUniversity of Michigan▼bElectrical and Computer Engineering.
■7730 ▼tDissertations Abstracts International▼g85-12B.
■790 ▼a0127
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17162787▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
