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Exploring De Novo Protein Functionalities: Pyridoxal-5'-Phosphate-Dependent Catalysis and Quantum Dot Synthesis
Exploring De Novo Protein Functionalities: Pyridoxal-5'-Phosphate-Dependent Catalysis and Quantum Dot Synthesis
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211152823
- ISBN
- 9798384464006
- DDC
- 540
- 저자명
- Yao, Yueyu.
- 서명/저자
- Exploring De Novo Protein Functionalities: Pyridoxal-5-Phosphate-Dependent Catalysis and Quantum Dot Synthesis
- 발행사항
- [Sl] : Princeton University, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 185 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 86-04, Section: B.
- 주기사항
- Advisor: Hecht, Michael H.
- 학위논문주기
- Thesis (Ph.D.)--Princeton University, 2024.
- 초록/해제
- 요약This dissertation explores the potential of de novo proteins, created through binary patterning. These novel sequences revealed significant functional capabilities, including PLP-dependent catalysis. Notably, de novo proteins were found to bind pyridoxal-5'- phosphate and catalyze reactions like cysteine desulfhydration, opening avenues for engineering enzymes with diverse PLP-dependent activities.Later chapters highlight the application of de novo proteins in nanoparticle fabrication. Chapter 3 presents the biomineralization of cadmium sulfide nanocrystals using the de novo protein ConK, which produces H2S to react with the cadmium precursor, forming CdS quantum dots. Chapter 4 investigates the structural role of de novo proteins in quantum dot formation. Four proteins were found to cap CdS quantum dots effectively, ensuring precise size control and distinct optical properties. These findings suggest that applications of de novo proteins extend beyond biological functions.In conclusion, this dissertation identifies functional de novo proteins that catalyze critical reactions and interact with metals, demonstrating the potential within the protein sequence space. It lays the groundwork for future research at the intersection of synthetic biology and material science, offering starting points for further exploration into this uncharted territory.
- 일반주제명
- Chemistry
- 일반주제명
- Materials science
- 일반주제명
- Nanoscience
- 키워드
- Protein design
- 키워드
- Quantum dots
- 기타저자
- Princeton University Chemistry
- 기본자료저록
- Dissertations Abstracts International. 86-04B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■020 ▼a9798384464006
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■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a540
■1001 ▼aYao, Yueyu.▼0(orcid)0000-0003-3832-0125
■24510▼aExploring De Novo Protein Functionalities: Pyridoxal-5'-Phosphate-Dependent Catalysis and Quantum Dot Synthesis
■260 ▼a[Sl]▼bPrinceton University▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a185 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-04, Section: B.
■500 ▼aAdvisor: Hecht, Michael H.
■5021 ▼aThesis (Ph.D.)--Princeton University, 2024.
■520 ▼aThis dissertation explores the potential of de novo proteins, created through binary patterning. These novel sequences revealed significant functional capabilities, including PLP-dependent catalysis. Notably, de novo proteins were found to bind pyridoxal-5'- phosphate and catalyze reactions like cysteine desulfhydration, opening avenues for engineering enzymes with diverse PLP-dependent activities.Later chapters highlight the application of de novo proteins in nanoparticle fabrication. Chapter 3 presents the biomineralization of cadmium sulfide nanocrystals using the de novo protein ConK, which produces H2S to react with the cadmium precursor, forming CdS quantum dots. Chapter 4 investigates the structural role of de novo proteins in quantum dot formation. Four proteins were found to cap CdS quantum dots effectively, ensuring precise size control and distinct optical properties. These findings suggest that applications of de novo proteins extend beyond biological functions.In conclusion, this dissertation identifies functional de novo proteins that catalyze critical reactions and interact with metals, demonstrating the potential within the protein sequence space. It lays the groundwork for future research at the intersection of synthetic biology and material science, offering starting points for further exploration into this uncharted territory.
■590 ▼aSchool code: 0181.
■650 4▼aChemistry
■650 4▼aMaterials science
■650 4▼aNanoscience
■653 ▼aBiomineralization
■653 ▼aProtein design
■653 ▼aQuantum dots
■653 ▼aCysteine desulfhydration
■653 ▼aSynthetic biology
■690 ▼a0485
■690 ▼a0565
■690 ▼a0794
■71020▼aPrinceton University▼bChemistry.
■7730 ▼tDissertations Abstracts International▼g86-04B.
■790 ▼a0181
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17164031▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
