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A Ubiquitous GC Content Signature Underlies Multimodal mRNA Regulation by DDX3X
A Ubiquitous GC Content Signature Underlies Multimodal mRNA Regulation by DDX3X
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211151120
- ISBN
- 9798382816104
- DDC
- 574
- 서명/저자
- A Ubiquitous GC Content Signature Underlies Multimodal mRNA Regulation by DDX3X
- 발행사항
- [Sl] : University of California, San Francisco, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 83 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 85-12, Section: B.
- 주기사항
- Includes supplementary digital materials.
- 주기사항
- Advisor: Weiss, William.
- 학위논문주기
- Thesis (Ph.D.)--University of California, San Francisco, 2024.
- 초록/해제
- 요약DDX3X encodes for a DEAD-box RNA helicase that promotes the translation of mRNAs that contain long, highly structured 5' UTRs. DDX3X interacts with translation initiation factors and can remodel mRNA structures or RNA protein complexes in an ATP-dependent manner. Our prior work found that DDX3X depletion leads to changes in translation and RNA levels while missense variants selectively impact translation, implicating DDX3X in regulating mRNA stability. Despite being an essential gene and implicated in several human cancers and developmental diseases, including intellectual disability and autism spectrum disorder, the mechanisms of DDX3X in post-transcriptional gene regulation and mRNA metabolism remain poorly understood. To address this gap in knowledge in DDX3X function in post-transcriptional gene regulation and mRNA metabolism, this work studied the mechanism of how DDX3X regulates specific mRNA transcripts and confers stability or loss of stability and how this process is altered with loss of DDX3X. DDX3X's function in translation is well characterized, is known to be involved in nuclear export of specific mRNA, and our preliminary data demonstrates that DDX3X specific transcripts have altered stability. However, what features and characteristics of these DDX3X-dependent transcripts are key for DDX3X driven stability remain unknown. This study aimed to define what features of mRNA transcripts are key for how DDX3X regulates RNA metabolism both at the level of translation and transcript stability. This knowledge is critical for advancing our knowledge in post-transcriptional gene regulation and mRNA metabolism.
- 일반주제명
- Molecular biology
- 일반주제명
- Bioinformatics
- 일반주제명
- Cellular biology
- 일반주제명
- Genetics
- 키워드
- mRNA metabolism
- 키워드
- Translation
- 기타저자
- University of California, San Francisco Biomedical Sciences
- 기본자료저록
- Dissertations Abstracts International. 85-12B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■00520250211151120
■006m o d
■007cr#unu||||||||
■020 ▼a9798382816104
■035 ▼a(MiAaPQ)AAI31146070
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574
■1001 ▼aJowhar, Ziad Mohamoud.▼0(orcid)0000-0002-3536-079
■24512▼aA Ubiquitous GC Content Signature Underlies Multimodal mRNA Regulation by DDX3X
■260 ▼a[Sl]▼bUniversity of California, San Francisco▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a83 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 85-12, Section: B.
■500 ▼aIncludes supplementary digital materials.
■500 ▼aAdvisor: Weiss, William.
■5021 ▼aThesis (Ph.D.)--University of California, San Francisco, 2024.
■520 ▼aDDX3X encodes for a DEAD-box RNA helicase that promotes the translation of mRNAs that contain long, highly structured 5' UTRs. DDX3X interacts with translation initiation factors and can remodel mRNA structures or RNA protein complexes in an ATP-dependent manner. Our prior work found that DDX3X depletion leads to changes in translation and RNA levels while missense variants selectively impact translation, implicating DDX3X in regulating mRNA stability. Despite being an essential gene and implicated in several human cancers and developmental diseases, including intellectual disability and autism spectrum disorder, the mechanisms of DDX3X in post-transcriptional gene regulation and mRNA metabolism remain poorly understood. To address this gap in knowledge in DDX3X function in post-transcriptional gene regulation and mRNA metabolism, this work studied the mechanism of how DDX3X regulates specific mRNA transcripts and confers stability or loss of stability and how this process is altered with loss of DDX3X. DDX3X's function in translation is well characterized, is known to be involved in nuclear export of specific mRNA, and our preliminary data demonstrates that DDX3X specific transcripts have altered stability. However, what features and characteristics of these DDX3X-dependent transcripts are key for DDX3X driven stability remain unknown. This study aimed to define what features of mRNA transcripts are key for how DDX3X regulates RNA metabolism both at the level of translation and transcript stability. This knowledge is critical for advancing our knowledge in post-transcriptional gene regulation and mRNA metabolism.
■590 ▼aSchool code: 0034.
■650 4▼aMolecular biology
■650 4▼aBioinformatics
■650 4▼aCellular biology
■650 4▼aGenetics
■653 ▼aPost-transcriptional gene regulation
■653 ▼aIntellectual disability
■653 ▼amRNA metabolism
■653 ▼aTranslation
■690 ▼a0307
■690 ▼a0715
■690 ▼a0379
■690 ▼a0369
■71020▼aUniversity of California, San Francisco▼bBiomedical Sciences.
■7730 ▼tDissertations Abstracts International▼g85-12B.
■790 ▼a0034
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17160806▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
