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Role of Alveolar Epithelial Cells and Macrophages in the Pathogenesis of Respiratory Viral Infections: Computational Approach Using Transcriptomics
Role of Alveolar Epithelial Cells and Macrophages in the Pathogenesis of Respiratory Viral Infections: Computational Approach Using Transcriptomics
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211151016
- ISBN
- 9798382762845
- DDC
- 574
- 서명/저자
- Role of Alveolar Epithelial Cells and Macrophages in the Pathogenesis of Respiratory Viral Infections: Computational Approach Using Transcriptomics
- 발행사항
- [Sl] : Northwestern University, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 172 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 85-11, Section: B.
- 주기사항
- Advisor: Ridge, Karen M.
- 학위논문주기
- Thesis (Ph.D.)--Northwestern University, 2024.
- 초록/해제
- 요약This study utilizes RNA sequencing (RNA-seq) to probe the juvenile response to influenza A virus (IAV) infection, with a specific focus on key cellular groups: Alveolar Epithelial Cells (AECs), Tissue Resident Alveolar Macrophages (TRAMs), and Monocyte-Derived Alveolar Macrophages (MoAMs). Although the impact of IAV in adults has been extensively studied, the response in juveniles remains less characterized. We aimed to unravel the transcriptional dynamics and intercellular communication of these cells during juvenile IAV infection to identify unique molecular signatures and pathways.Challenging the conventional use of plaque assays as the primary method for measuring viral titers, our study combines this technique with viral transcript measurements. This dual approach not only provides a more comprehensive understanding of the viral presence in the host but also emphasizes the necessity for broader metrics in viral infection assessment. Our comprehensive RNA-seq analysis uncovered distinct, time-specific transcriptomic responses in juveniles, underscoring critical pathways and regulatory networks vital for counteracting IAV. A key finding is the intricate cell-to-cell communication within the Type-1 IFN-related pathway, which plays a crucial role in the juvenile immune response. Disruption of Type-1 IFN signaling in MoAMs through the IFN receptor significantly improved survival rates in juvenile mice, opening up promising avenues for therapeutic intervention.This research advances our understanding of pediatric influenza, highlighting the complexity of the juvenile immune response to IAV and advocating for more nuanced and expansive approaches in viral infection studies. The insights gained from our study have important implications for developing targeted treatments to alleviate the effects of IAV in younger populations.
- 일반주제명
- Bioinformatics
- 일반주제명
- Biomedical engineering
- 일반주제명
- Molecular biology
- 일반주제명
- Genetics
- 일반주제명
- Pathology
- 키워드
- RNA sequencing
- 기타저자
- Northwestern University Driskill Graduate Training Program in Life Sciences
- 기본자료저록
- Dissertations Abstracts International. 85-11B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■007cr#unu||||||||
■020 ▼a9798382762845
■035 ▼a(MiAaPQ)AAI30995869
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574
■1001 ▼aAnekalla, Kishore Reddy.
■24510▼aRole of Alveolar Epithelial Cells and Macrophages in the Pathogenesis of Respiratory Viral Infections: Computational Approach Using Transcriptomics
■260 ▼a[Sl]▼bNorthwestern University▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a172 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 85-11, Section: B.
■500 ▼aAdvisor: Ridge, Karen M.
■5021 ▼aThesis (Ph.D.)--Northwestern University, 2024.
■520 ▼aThis study utilizes RNA sequencing (RNA-seq) to probe the juvenile response to influenza A virus (IAV) infection, with a specific focus on key cellular groups: Alveolar Epithelial Cells (AECs), Tissue Resident Alveolar Macrophages (TRAMs), and Monocyte-Derived Alveolar Macrophages (MoAMs). Although the impact of IAV in adults has been extensively studied, the response in juveniles remains less characterized. We aimed to unravel the transcriptional dynamics and intercellular communication of these cells during juvenile IAV infection to identify unique molecular signatures and pathways.Challenging the conventional use of plaque assays as the primary method for measuring viral titers, our study combines this technique with viral transcript measurements. This dual approach not only provides a more comprehensive understanding of the viral presence in the host but also emphasizes the necessity for broader metrics in viral infection assessment. Our comprehensive RNA-seq analysis uncovered distinct, time-specific transcriptomic responses in juveniles, underscoring critical pathways and regulatory networks vital for counteracting IAV. A key finding is the intricate cell-to-cell communication within the Type-1 IFN-related pathway, which plays a crucial role in the juvenile immune response. Disruption of Type-1 IFN signaling in MoAMs through the IFN receptor significantly improved survival rates in juvenile mice, opening up promising avenues for therapeutic intervention.This research advances our understanding of pediatric influenza, highlighting the complexity of the juvenile immune response to IAV and advocating for more nuanced and expansive approaches in viral infection studies. The insights gained from our study have important implications for developing targeted treatments to alleviate the effects of IAV in younger populations.
■590 ▼aSchool code: 0163.
■650 4▼aBioinformatics
■650 4▼aBiomedical engineering
■650 4▼aMolecular biology
■650 4▼aGenetics
■650 4▼aPathology
■653 ▼aRNA sequencing
■653 ▼aInfluenza A virus
■653 ▼aTissue Resident Alveolar Macrophages
■653 ▼aAlveolar Epithelial Cells
■653 ▼aMonocyte-Derived Alveolar Macrophages
■690 ▼a0715
■690 ▼a0541
■690 ▼a0369
■690 ▼a0307
■690 ▼a0571
■71020▼aNorthwestern University▼bDriskill Graduate Training Program in Life Sciences.
■7730 ▼tDissertations Abstracts International▼g85-11B.
■790 ▼a0163
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17160418▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
