서브메뉴
검색
Epithelial Biophysics at Molecular and Sub-Tissue Scales
Epithelial Biophysics at Molecular and Sub-Tissue Scales
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211152118
- ISBN
- 9798384336495
- DDC
- 612
- 서명/저자
- Epithelial Biophysics at Molecular and Sub-Tissue Scales
- 발행사항
- [Sl] : Stanford University, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 104 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 86-03, Section: B.
- 주기사항
- Advisor: Dunn, Alexander.
- 학위논문주기
- Thesis (Ph.D.)--Stanford University, 2024.
- 초록/해제
- 요약Cell-cell adhesion and apicobasal polarity are defining characteristics of epithelial tissues that enabled the development of multicellular life. In this thesis, I describe two projects that aim to address questions about each of these fundamental processes.First, I present work using live-cell imaging to study the physical mechanism of lumen formation in Madin-Darby Canine Kidney (MDCK) cell spheroids, a canonical cell-culture model for lumenogenesis. We find that in this system, lumen shape reflects basic geometrical considerations tied to the establishment of apico-basal polarity. A physical model incorporating both cell geometry and intraluminal pressure can account for our observations as well as cases in which pressure plays a dominant role.Next, I present a study characterizing the single-molecule mechanics of an understudied class of cell adhesion molecules. Afadin is an essential actin-binding protein that is associated with both adherens and tight junctions. Using a single-molecule magnetic tweezers assay, we probed the mechanical stability of the bonds between the Afadin PDZ domain and the intracellular domains of two of its binding partners, Nectin-1 and JAM-A. We found that both ligands formed remarkably stable bonds with Afadin-PDZ at forces up to 10 pN. Our data suggest that the relatively-understudied complexes of afadin with JAM-A and Nectin-1 may function in parallel with cadherin-based adhesions to transmit forces across cell-cell junctions.
- 일반주제명
- Physiology
- 일반주제명
- Pathogens
- 일반주제명
- Fourier transforms
- 일반주제명
- Disease
- 일반주제명
- Permeability
- 일반주제명
- Microscopy
- 일반주제명
- Extracellular matrix
- 일반주제명
- Spheroids
- 일반주제명
- Bond strength
- 일반주제명
- Morphogenesis
- 일반주제명
- Biophysics
- 일반주제명
- Cellular biology
- 일반주제명
- Developmental biology
- 일반주제명
- Mathematics
- 기타저자
- Stanford University.
- 기본자료저록
- Dissertations Abstracts International. 86-03B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
008250123s2024 us c eng d■001000017162973
■00520250211152118
■006m o d
■007cr#unu||||||||
■020 ▼a9798384336495
■035 ▼a(MiAaPQ)AAI31460353
■035 ▼a(MiAaPQ)Stanfordsq687jx1202
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a612
■1001 ▼aVachharajani, Vipul Tushar.
■24510▼aEpithelial Biophysics at Molecular and Sub-Tissue Scales
■260 ▼a[Sl]▼bStanford University▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a104 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-03, Section: B.
■500 ▼aAdvisor: Dunn, Alexander.
■5021 ▼aThesis (Ph.D.)--Stanford University, 2024.
■520 ▼aCell-cell adhesion and apicobasal polarity are defining characteristics of epithelial tissues that enabled the development of multicellular life. In this thesis, I describe two projects that aim to address questions about each of these fundamental processes.First, I present work using live-cell imaging to study the physical mechanism of lumen formation in Madin-Darby Canine Kidney (MDCK) cell spheroids, a canonical cell-culture model for lumenogenesis. We find that in this system, lumen shape reflects basic geometrical considerations tied to the establishment of apico-basal polarity. A physical model incorporating both cell geometry and intraluminal pressure can account for our observations as well as cases in which pressure plays a dominant role.Next, I present a study characterizing the single-molecule mechanics of an understudied class of cell adhesion molecules. Afadin is an essential actin-binding protein that is associated with both adherens and tight junctions. Using a single-molecule magnetic tweezers assay, we probed the mechanical stability of the bonds between the Afadin PDZ domain and the intracellular domains of two of its binding partners, Nectin-1 and JAM-A. We found that both ligands formed remarkably stable bonds with Afadin-PDZ at forces up to 10 pN. Our data suggest that the relatively-understudied complexes of afadin with JAM-A and Nectin-1 may function in parallel with cadherin-based adhesions to transmit forces across cell-cell junctions.
■590 ▼aSchool code: 0212.
■650 4▼aPhysiology
■650 4▼aPathogens
■650 4▼aFourier transforms
■650 4▼aCell adhesion & migration
■650 4▼aDisease
■650 4▼aPermeability
■650 4▼aMicroscopy
■650 4▼aExtracellular matrix
■650 4▼aSpheroids
■650 4▼aBond strength
■650 4▼aMorphogenesis
■650 4▼aBiophysics
■650 4▼aCellular biology
■650 4▼aDevelopmental biology
■650 4▼aMathematics
■690 ▼a0719
■690 ▼a0786
■690 ▼a0379
■690 ▼a0758
■690 ▼a0405
■71020▼aStanford University.
■7730 ▼tDissertations Abstracts International▼g86-03B.
■790 ▼a0212
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17162973▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
