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Mapping Bacteria and Mobile Genetic Elements in Human Microbiomes
Mapping Bacteria and Mobile Genetic Elements in Human Microbiomes
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211152132
- ISBN
- 9798384049593
- DDC
- 610
- 서명/저자
- Mapping Bacteria and Mobile Genetic Elements in Human Microbiomes
- 발행사항
- [Sl] : Cornell University, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 117 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 86-03, Section: B.
- 주기사항
- Includes supplementary digital materials.
- 주기사항
- Advisor: De Vlaminck, Iwijn.
- 학위논문주기
- Thesis (Ph.D.)--Cornell University, 2024.
- 초록/해제
- 요약The complex microbial communities that comprise the human microbiome form intricate spatially structured biofilms on host surfaces, creating networks of interactions and modulating human health. Yet progress in understanding these microscale ecosystems has historically been hindered by limited tools to spatially map the full complexity of microbial communities. We reviewed recent advances in tools to spatially map microbiomes, identifying fluorescence in situ hybridization (FISH) as a particularly promising approach. We then developed an imaging approach that pairs single molecule DNA-FISH with multiplexed ribosomal RNA-FISH, thereby enabling the simultaneous visualization of both mobile genetic elements and their cognate hosts. We revealed that spatial heterogeneity in bacterial taxa results in heterogenous distribution of MGEs within the community, where clusters of MGEs could be local hotspots of horizontal gene transfer or expansion of host strains carrying the MGE. Last, we applied bacterial taxon mapping to study dysbiosis in peri-implant disease. Based on our findings, we propose a model of peri-implant dysbiosis where changes in the spatial structure allow colonization of new community members that provoke the immune system. The methods introduced here can help advance the study of microbial ecology and address pressing questions regarding dysbiosis, antimicrobial resistance, and phage therapy.
- 일반주제명
- Biomedical engineering
- 일반주제명
- Microbiology
- 일반주제명
- Ecology
- 일반주제명
- Genetics
- 키워드
- Biofilms
- 키워드
- Dysbiosis
- 키워드
- Imaging
- 키워드
- Microbiome
- 키워드
- Spatial
- 기타저자
- Cornell University Biomedical Engineering
- 기본자료저록
- Dissertations Abstracts International. 86-03B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■00520250211152132
■006m o d
■007cr#unu||||||||
■020 ▼a9798384049593
■035 ▼a(MiAaPQ)AAI31483518
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a610
■1001 ▼aGrodner, Benjamin Michael.▼0(orcid)0000-0001-7679-2129
■24510▼aMapping Bacteria and Mobile Genetic Elements in Human Microbiomes
■260 ▼a[Sl]▼bCornell University▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a117 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-03, Section: B.
■500 ▼aIncludes supplementary digital materials.
■500 ▼aAdvisor: De Vlaminck, Iwijn.
■5021 ▼aThesis (Ph.D.)--Cornell University, 2024.
■520 ▼aThe complex microbial communities that comprise the human microbiome form intricate spatially structured biofilms on host surfaces, creating networks of interactions and modulating human health. Yet progress in understanding these microscale ecosystems has historically been hindered by limited tools to spatially map the full complexity of microbial communities. We reviewed recent advances in tools to spatially map microbiomes, identifying fluorescence in situ hybridization (FISH) as a particularly promising approach. We then developed an imaging approach that pairs single molecule DNA-FISH with multiplexed ribosomal RNA-FISH, thereby enabling the simultaneous visualization of both mobile genetic elements and their cognate hosts. We revealed that spatial heterogeneity in bacterial taxa results in heterogenous distribution of MGEs within the community, where clusters of MGEs could be local hotspots of horizontal gene transfer or expansion of host strains carrying the MGE. Last, we applied bacterial taxon mapping to study dysbiosis in peri-implant disease. Based on our findings, we propose a model of peri-implant dysbiosis where changes in the spatial structure allow colonization of new community members that provoke the immune system. The methods introduced here can help advance the study of microbial ecology and address pressing questions regarding dysbiosis, antimicrobial resistance, and phage therapy.
■590 ▼aSchool code: 0058.
■650 4▼aBiomedical engineering
■650 4▼aMicrobiology
■650 4▼aEcology
■650 4▼aGenetics
■653 ▼aBiofilms
■653 ▼aDysbiosis
■653 ▼aImaging
■653 ▼aMicrobiome
■653 ▼aMobile genetic elements
■653 ▼aSpatial
■690 ▼a0541
■690 ▼a0410
■690 ▼a0369
■690 ▼a0329
■71020▼aCornell University▼bBiomedical Engineering.
■7730 ▼tDissertations Abstracts International▼g86-03B.
■790 ▼a0058
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17163077▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
