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De Novo Design of Protein Binders to Bioactive Peptides: From Hormones to Snake Toxins
De Novo Design of Protein Binders to Bioactive Peptides: From Hormones to Snake Toxins
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211152755
- ISBN
- 9798384093794
- DDC
- 574
- 서명/저자
- De Novo Design of Protein Binders to Bioactive Peptides: From Hormones to Snake Toxins
- 발행사항
- [Sl] : University of Washington, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 115 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 86-03, Section: B.
- 주기사항
- Advisor: Baker, David.
- 학위논문주기
- Thesis (Ph.D.)--University of Washington, 2024.
- 초록/해제
- 요약Recent advancements in deep learning methods for protein design and structure prediction have demonstrated the potential of de novo proteins to replace antibodies as affinity reagents, offering cost-effective production, increased stability, and robust platforms for therapeutics and diagnostics.In this work, we set out to design de novo protein binders for two classes of bioactive peptides: hormones which adopt α-helical structures, and lethal snake toxins from the three-finger toxin (3FTX) family. Using deep learning-based methods, we generated high-affinity binders for both targets. These binders enable hormone detection via mass spectrometry and the creation of bioluminescence-based biosensors. Additionally, our designed proteins effectively neutralize 3FTXs in vitro and protect mice from lethal neurotoxin challenges.While further development is necessary to translate these designs into practical diagnostics and therapeutics, this work demonstrates their potential as robust reagents for hormone detection and as safer, widely accessible antivenoms.
- 일반주제명
- Biochemistry
- 일반주제명
- Physiology
- 일반주제명
- Toxicology
- 키워드
- Deep learning
- 키워드
- Biosensors
- 기타저자
- University of Washington Biochemistry
- 기본자료저록
- Dissertations Abstracts International. 86-03B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■00520250211152755
■006m o d
■007cr#unu||||||||
■020 ▼a9798384093794
■035 ▼a(MiAaPQ)AAI31555760
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574
■1001 ▼aVazquez Torres, Susana.
■24510▼aDe Novo Design of Protein Binders to Bioactive Peptides: From Hormones to Snake Toxins
■260 ▼a[Sl]▼bUniversity of Washington▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a115 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-03, Section: B.
■500 ▼aAdvisor: Baker, David.
■5021 ▼aThesis (Ph.D.)--University of Washington, 2024.
■520 ▼aRecent advancements in deep learning methods for protein design and structure prediction have demonstrated the potential of de novo proteins to replace antibodies as affinity reagents, offering cost-effective production, increased stability, and robust platforms for therapeutics and diagnostics.In this work, we set out to design de novo protein binders for two classes of bioactive peptides: hormones which adopt α-helical structures, and lethal snake toxins from the three-finger toxin (3FTX) family. Using deep learning-based methods, we generated high-affinity binders for both targets. These binders enable hormone detection via mass spectrometry and the creation of bioluminescence-based biosensors. Additionally, our designed proteins effectively neutralize 3FTXs in vitro and protect mice from lethal neurotoxin challenges.While further development is necessary to translate these designs into practical diagnostics and therapeutics, this work demonstrates their potential as robust reagents for hormone detection and as safer, widely accessible antivenoms.
■590 ▼aSchool code: 0250.
■650 4▼aBiochemistry
■650 4▼aPhysiology
■650 4▼aToxicology
■653 ▼aDeep learning
■653 ▼aThree-finger toxin
■653 ▼aBiosensors
■653 ▼aBioactive peptides
■690 ▼a0487
■690 ▼a0383
■690 ▼a0719
■71020▼aUniversity of Washington▼bBiochemistry.
■7730 ▼tDissertations Abstracts International▼g86-03B.
■790 ▼a0250
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17163800▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
