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An Investigation of Microtubule-Kinetochore Attachment Mechanisms
An Investigation of Microtubule-Kinetochore Attachment Mechanisms
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211151028
- ISBN
- 9798382215389
- DDC
- 574.191
- 서명/저자
- An Investigation of Microtubule-Kinetochore Attachment Mechanisms
- 발행사항
- [Sl] : University of Washington, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 126 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 85-10, Section: B.
- 주기사항
- Advisor: Asbury, Chip.
- 학위논문주기
- Thesis (Ph.D.)--University of Washington, 2024.
- 초록/해제
- 요약The ability to replicate is a defining feature of life. At the center of eukaryotic cell division are a set of protein machines responsible for pulling apart the chromosomes before cells divide. Spindle microtubules grow from the poles of the cell and connect to chromosomes via protein complexes called kinetochores. Kinetochores must maintain tenacious attachments to microtubule tips, even as they assemble and disassemble underneath their grip. Additionally, kinetochores mediate an error correction process to ensure the proper attachments to microtubules are formed before separation of the chromosomes commences. Here, I work to understand how the proteins in the kinetochore work together to maintain attachments to microtubules. I investigate two different mechanisms for microtubule-kinetochore attachment: the conformational wave mechanism and the biased diffusion mechanism. I developed a new optical trapping assay, using it to show that microtubule protofilament morphological and energetic properties can be measured and changed. I investigate the role of protofilament curl enlargement in the attachment and motility of the kinetochore. I develop theoretical models that show that the biased diffusion mechanism can fit experimentally measured detachment rates for assembling and disassembling kinetochores. Finally, I show kinetochores exhibit asymmetry in their sliding friction when they are dragged along microtubule lattices, a new phenomenon for microtubule-kinetochore biophysics. I argue this sliding friction forms the basis for a new mode of error correction during cell division, one that likely holds across most eukaryotic organisms.
- 일반주제명
- Biophysics
- 일반주제명
- Physiology
- 일반주제명
- Cellular biology
- 일반주제명
- Morphology
- 키워드
- Cell division
- 키워드
- Kinetochores
- 키워드
- Microtubules
- 키워드
- Mitosis
- 키워드
- Optical trapping
- 기타저자
- University of Washington Physiology and Biophysics
- 기본자료저록
- Dissertations Abstracts International. 85-10B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■00520250211151028
■006m o d
■007cr#unu||||||||
■020 ▼a9798382215389
■035 ▼a(MiAaPQ)AAI30997090
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574.191
■1001 ▼aMurray, Lucas Edward.
■24513▼aAn Investigation of Microtubule-Kinetochore Attachment Mechanisms
■260 ▼a[Sl]▼bUniversity of Washington▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a126 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 85-10, Section: B.
■500 ▼aAdvisor: Asbury, Chip.
■5021 ▼aThesis (Ph.D.)--University of Washington, 2024.
■520 ▼aThe ability to replicate is a defining feature of life. At the center of eukaryotic cell division are a set of protein machines responsible for pulling apart the chromosomes before cells divide. Spindle microtubules grow from the poles of the cell and connect to chromosomes via protein complexes called kinetochores. Kinetochores must maintain tenacious attachments to microtubule tips, even as they assemble and disassemble underneath their grip. Additionally, kinetochores mediate an error correction process to ensure the proper attachments to microtubules are formed before separation of the chromosomes commences. Here, I work to understand how the proteins in the kinetochore work together to maintain attachments to microtubules. I investigate two different mechanisms for microtubule-kinetochore attachment: the conformational wave mechanism and the biased diffusion mechanism. I developed a new optical trapping assay, using it to show that microtubule protofilament morphological and energetic properties can be measured and changed. I investigate the role of protofilament curl enlargement in the attachment and motility of the kinetochore. I develop theoretical models that show that the biased diffusion mechanism can fit experimentally measured detachment rates for assembling and disassembling kinetochores. Finally, I show kinetochores exhibit asymmetry in their sliding friction when they are dragged along microtubule lattices, a new phenomenon for microtubule-kinetochore biophysics. I argue this sliding friction forms the basis for a new mode of error correction during cell division, one that likely holds across most eukaryotic organisms.
■590 ▼aSchool code: 0250.
■650 4▼aBiophysics
■650 4▼aPhysiology
■650 4▼aCellular biology
■650 4▼aMorphology
■653 ▼aCell division
■653 ▼aKinetochores
■653 ▼aMicrotubules
■653 ▼aMitosis
■653 ▼aOptical trapping
■690 ▼a0786
■690 ▼a0379
■690 ▼a0287
■690 ▼a0719
■71020▼aUniversity of Washington▼bPhysiology and Biophysics.
■7730 ▼tDissertations Abstracts International▼g85-10B.
■790 ▼a0250
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17160490▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
