Dynamics of the Artificial Axon
Dynamics of the Artificial Axon
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211151048
- ISBN
- 9798381968309
- DDC
- 574.191
- 저자명
- Pi, Ziqi.
- 서명/저자
- Dynamics of the Artificial Axon
- 발행사항
- [Sl] : University of California, Los Angeles, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 140 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 85-09, Section: B.
- 주기사항
- Advisor: Zocchi, Giovanni.
- 학위논문주기
- Thesis (Ph.D.)--University of California, Los Angeles, 2024.
- 초록/해제
- 요약The "Artificial Axon" (AA) is a synthetic excitable system developed in the Zocchi lab, constructed with the minimal biological components. Based on traditional black lipid membrane setups, the AA is the first cell-free platform capable of producing action potentials (APs) in time. This dissertation details the results of my work with the AA, using the voltage gated potassium channel KvAP as the active ingredient. First, I report on experimental measurements with the AA near the threshold for firing APs, a critical point of the system. In particular, a delay in firing occurs due to the presence of a saddle node bifurcation, and a scaling exponent for this delay is measured. Supplemented by numerical results, I show that this behavior near the critical point has correspondence to the real neuron, due to the universal nature of the dynamics near a critical point. Next, I will characterize the AA in terms of its phenomenology. Using a minimal 3D model based on the Hodgkin-Huxley model, I construct a qualitative phase diagram in the parameter space of the system. The existence of limit cycle regions in this phase diagram indicates that the AA is capable of generating self sustaining AP trains with just a single ion channel. The analysis also shows that the AA, having just one channel species with inactivation, possesses all the same dynamics as a two ion species system without inactivation, such as the Morris-Lecar model for the muscle fiber of the giant barnacle. This result is followed by measurements of the effective inactivation and recovery rates for our minimal model, qualitatively placing the AA with KvAP on the phase diagram. Finally, I will present work on an experimental system consisting of two AA connected by electronic "synapses", and explore the feasibility of constructing an autonomous oscillator with such a configuration. The connection of two AAs serves as a first step for our long term goal of AA based networks. The dissertation concludes with a short discussion on future directions for the Artificial Axon system.
- 일반주제명
- Biophysics
- 일반주제명
- Condensed matter physics
- 일반주제명
- Physics
- 키워드
- Action potential
- 키워드
- Dynamics
- 기타저자
- University of California, Los Angeles Physics 0666
- 기본자료저록
- Dissertations Abstracts International. 85-09B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
008250123s2024 us c eng d■001000017160607
■00520250211151048
■006m o d
■007cr#unu||||||||
■020 ▼a9798381968309
■035 ▼a(MiAaPQ)AAI31141031
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574.191
■1001 ▼aPi, Ziqi.
■24510▼aDynamics of the Artificial Axon
■260 ▼a[Sl]▼bUniversity of California, Los Angeles▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a140 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 85-09, Section: B.
■500 ▼aAdvisor: Zocchi, Giovanni.
■5021 ▼aThesis (Ph.D.)--University of California, Los Angeles, 2024.
■520 ▼aThe "Artificial Axon" (AA) is a synthetic excitable system developed in the Zocchi lab, constructed with the minimal biological components. Based on traditional black lipid membrane setups, the AA is the first cell-free platform capable of producing action potentials (APs) in time. This dissertation details the results of my work with the AA, using the voltage gated potassium channel KvAP as the active ingredient. First, I report on experimental measurements with the AA near the threshold for firing APs, a critical point of the system. In particular, a delay in firing occurs due to the presence of a saddle node bifurcation, and a scaling exponent for this delay is measured. Supplemented by numerical results, I show that this behavior near the critical point has correspondence to the real neuron, due to the universal nature of the dynamics near a critical point. Next, I will characterize the AA in terms of its phenomenology. Using a minimal 3D model based on the Hodgkin-Huxley model, I construct a qualitative phase diagram in the parameter space of the system. The existence of limit cycle regions in this phase diagram indicates that the AA is capable of generating self sustaining AP trains with just a single ion channel. The analysis also shows that the AA, having just one channel species with inactivation, possesses all the same dynamics as a two ion species system without inactivation, such as the Morris-Lecar model for the muscle fiber of the giant barnacle. This result is followed by measurements of the effective inactivation and recovery rates for our minimal model, qualitatively placing the AA with KvAP on the phase diagram. Finally, I will present work on an experimental system consisting of two AA connected by electronic "synapses", and explore the feasibility of constructing an autonomous oscillator with such a configuration. The connection of two AAs serves as a first step for our long term goal of AA based networks. The dissertation concludes with a short discussion on future directions for the Artificial Axon system.
■590 ▼aSchool code: 0031.
■650 4▼aBiophysics
■650 4▼aCondensed matter physics
■650 4▼aPhysics
■653 ▼aAction potential
■653 ▼aArtificial Axon system
■653 ▼aDynamics
■653 ▼aSynthetic biology
■653 ▼aMorris-Lecar model
■690 ▼a0786
■690 ▼a0611
■690 ▼a0605
■71020▼aUniversity of California, Los Angeles▼bPhysics 0666.
■7730 ▼tDissertations Abstracts International▼g85-09B.
■790 ▼a0031
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17160607▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
