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Technologies for the Cryopreservation of 3D Bioprinted Scaffolds
Technologies for the Cryopreservation of 3D Bioprinted Scaffolds
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211151435
- ISBN
- 9798384452959
- DDC
- 621
- 서명/저자
- Technologies for the Cryopreservation of 3D Bioprinted Scaffolds
- 발행사항
- [Sl] : University of California, Berkeley, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 82 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 86-03, Section: B.
- 주기사항
- Advisor: Rubinsky, Boris.
- 학위논문주기
- Thesis (Ph.D.)--University of California, Berkeley, 2024.
- 초록/해제
- 요약The use of 3D bioprinted scaffolds has many advantages over the use of 2D cell culture for modeling the human body, as significant evidence showsthat cells behave differently in 2D environments. Reliance on 2D cell culture during drug development contributes to high failure rate for new drugs. 3D bioprinted scaffolds are an alternative that can precisely mimic the 3D microenvironment of the body. However, the use of 3D bioprinting has been held back by the difficulty of cryopreserving 3D bioprinted scaffolds. Freezing a large, 3D scaffold creates an uneven temperature gradient and an unequal distribution of cryoprotectants, which compromises cell viability. This thesis presents "Temperature-Controlled-Cryoprinting" as a method of both fabricating and cryopreserving 3D bioprinted scaffolds. During Temperature-Controlled-Cryoprinting, a cellladen ink is printed on a freezing plate. As each layer is printed, the print plate descends further into a cooling bath, which ensures that all cells in the scaffold are frozen at the same rate. In Chapter 2 of this thesis, we explore the fundamentals of Temperature-Controlled-Cryoprinting, including the impact that freezing has on the mechanical and material properties of the scaffolds. In Chapter 3, we discuss the optimization of the 3D printing process and how to enhance scaffold stability with crosslinking. In Chapter 4, we discuss the advantages of Temperature-Controlled-Cryoprinting for the cryopreservation of 3D bioprinted scaffolds. Finally, in Chapter 5, we conclude with a discussion about the ways in which Temperature-Controlled-Cryoprinting could accelerate drug development and offer future perspectives.
- 일반주제명
- Mechanical engineering
- 일반주제명
- Biomedical engineering
- 일반주제명
- Bioengineering
- 키워드
- 3D bioprinting
- 키워드
- 3D cryoprinting
- 키워드
- Cryopreservation
- 기타저자
- University of California, Berkeley Mechanical Engineering
- 기본자료저록
- Dissertations Abstracts International. 86-03B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■00520250211151435
■006m o d
■007cr#unu||||||||
■020 ▼a9798384452959
■035 ▼a(MiAaPQ)AAI31295554
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a621
■1001 ▼aWarburton, Linnea.
■24510▼aTechnologies for the Cryopreservation of 3D Bioprinted Scaffolds
■260 ▼a[Sl]▼bUniversity of California, Berkeley▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a82 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-03, Section: B.
■500 ▼aAdvisor: Rubinsky, Boris.
■5021 ▼aThesis (Ph.D.)--University of California, Berkeley, 2024.
■520 ▼aThe use of 3D bioprinted scaffolds has many advantages over the use of 2D cell culture for modeling the human body, as significant evidence showsthat cells behave differently in 2D environments. Reliance on 2D cell culture during drug development contributes to high failure rate for new drugs. 3D bioprinted scaffolds are an alternative that can precisely mimic the 3D microenvironment of the body. However, the use of 3D bioprinting has been held back by the difficulty of cryopreserving 3D bioprinted scaffolds. Freezing a large, 3D scaffold creates an uneven temperature gradient and an unequal distribution of cryoprotectants, which compromises cell viability. This thesis presents "Temperature-Controlled-Cryoprinting" as a method of both fabricating and cryopreserving 3D bioprinted scaffolds. During Temperature-Controlled-Cryoprinting, a cellladen ink is printed on a freezing plate. As each layer is printed, the print plate descends further into a cooling bath, which ensures that all cells in the scaffold are frozen at the same rate. In Chapter 2 of this thesis, we explore the fundamentals of Temperature-Controlled-Cryoprinting, including the impact that freezing has on the mechanical and material properties of the scaffolds. In Chapter 3, we discuss the optimization of the 3D printing process and how to enhance scaffold stability with crosslinking. In Chapter 4, we discuss the advantages of Temperature-Controlled-Cryoprinting for the cryopreservation of 3D bioprinted scaffolds. Finally, in Chapter 5, we conclude with a discussion about the ways in which Temperature-Controlled-Cryoprinting could accelerate drug development and offer future perspectives.
■590 ▼aSchool code: 0028.
■650 4▼aMechanical engineering
■650 4▼aBiomedical engineering
■650 4▼aBioengineering
■653 ▼a3D bioprinting
■653 ▼a3D cryoprinting
■653 ▼aCryopreservation
■653 ▼aTissue engineering
■690 ▼a0548
■690 ▼a0541
■690 ▼a0202
■71020▼aUniversity of California, Berkeley▼bMechanical Engineering.
■7730 ▼tDissertations Abstracts International▼g86-03B.
■790 ▼a0028
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17161716▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
