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Differential Effects of G3BP Isoforms on Stress Granule Assembly and Gene Expression During Cellular Stress
Differential Effects of G3BP Isoforms on Stress Granule Assembly and Gene Expression During Cellular Stress
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211151156
- ISBN
- 9798382815527
- DDC
- 574
- 서명/저자
- Differential Effects of G3BP Isoforms on Stress Granule Assembly and Gene Expression During Cellular Stress
- 발행사항
- [Sl] : University of California, San Francisco, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 89 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 85-12, Section: B.
- 주기사항
- Advisor: Dumont, Sophie.
- 학위논문주기
- Thesis (Ph.D.)--University of California, San Francisco, 2024.
- 초록/해제
- 요약Stress granules (SGs) are macromolecular assemblies that form under cellular stress. Formation of these condensates is driven by the condensation of RNA and RNA-binding proteins such as G3BPs. G3BPs condense into SGs following stress-induced translational arrest. Three G3BP paralogs (G3BP1, G3BP2A, and G3BP2B) have been identified in vertebrates. However, the contribution of different G3BP paralogs to stress granule formation and stress-induced gene expression changes is incompletely understood. Here, we identified key residues for G3BP condensation such as V11. This conserved amino acid is required for formation of the G3BP-Caprin-1 complex, hence promoting SG assembly. Total RNA sequencing and ribosome profiling revealed that disruption of G3BP condensation corresponds to changes in mRNA levels and ribosome engagement during the integrated stress response (ISR). Moreover, we found that G3BP2B preferentially condenses and promotes changes in mRNA expression under endoplasmic reticulum (ER) stress. Together, this work suggests that stress granule assembly promotes changes in gene expression under cellular stress, which is differentially regulated by G3BP paralogs.
- 일반주제명
- Molecular biology
- 일반주제명
- Cellular biology
- 일반주제명
- Biochemistry
- 일반주제명
- Genetics
- 키워드
- G3BPs
- 키워드
- Stress granules
- 기타저자
- University of California, San Francisco Biochemistry and Molecular Biology
- 기본자료저록
- Dissertations Abstracts International. 85-12B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■020 ▼a9798382815527
■035 ▼a(MiAaPQ)AAI31236349
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574
■1001 ▼aLiboy Lugo, Jose.▼0(orcid)0000-0002-9997-2285
■24510▼aDifferential Effects of G3BP Isoforms on Stress Granule Assembly and Gene Expression During Cellular Stress
■260 ▼a[Sl]▼bUniversity of California, San Francisco▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a89 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 85-12, Section: B.
■500 ▼aAdvisor: Dumont, Sophie.
■5021 ▼aThesis (Ph.D.)--University of California, San Francisco, 2024.
■520 ▼aStress granules (SGs) are macromolecular assemblies that form under cellular stress. Formation of these condensates is driven by the condensation of RNA and RNA-binding proteins such as G3BPs. G3BPs condense into SGs following stress-induced translational arrest. Three G3BP paralogs (G3BP1, G3BP2A, and G3BP2B) have been identified in vertebrates. However, the contribution of different G3BP paralogs to stress granule formation and stress-induced gene expression changes is incompletely understood. Here, we identified key residues for G3BP condensation such as V11. This conserved amino acid is required for formation of the G3BP-Caprin-1 complex, hence promoting SG assembly. Total RNA sequencing and ribosome profiling revealed that disruption of G3BP condensation corresponds to changes in mRNA levels and ribosome engagement during the integrated stress response (ISR). Moreover, we found that G3BP2B preferentially condenses and promotes changes in mRNA expression under endoplasmic reticulum (ER) stress. Together, this work suggests that stress granule assembly promotes changes in gene expression under cellular stress, which is differentially regulated by G3BP paralogs.
■590 ▼aSchool code: 0034.
■650 4▼aMolecular biology
■650 4▼aCellular biology
■650 4▼aBiochemistry
■650 4▼aGenetics
■653 ▼aFluorescence microscopy
■653 ▼aG3BPs
■653 ▼aIntegrated stress response
■653 ▼aRibosome profiling
■653 ▼aStress granules
■690 ▼a0307
■690 ▼a0379
■690 ▼a0487
■690 ▼a0369
■71020▼aUniversity of California, San Francisco▼bBiochemistry and Molecular Biology.
■7730 ▼tDissertations Abstracts International▼g85-12B.
■790 ▼a0034
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17161058▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
