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High-Information Phenotypic Characterization of the Effect of Ionizing Radiation on Human Cells Using Rich Phenotypes From Perturb-Seq
High-Information Phenotypic Characterization of the Effect of Ionizing Radiation on Human Cells Using Rich Phenotypes From Perturb-Seq
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211153035
- ISBN
- 9798346876786
- DDC
- 574
- 저자명
- Wong, Garrett.
- 서명/저자
- High-Information Phenotypic Characterization of the Effect of Ionizing Radiation on Human Cells Using Rich Phenotypes From Perturb-Seq
- 발행사항
- [Sl] : University of California, San Francisco, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 52 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 86-06, Section: B.
- 주기사항
- Includes supplementary digital materials.
- 주기사항
- Advisor: Ntranos, Vasilis.
- 학위논문주기
- Thesis (Ph.D.)--University of California, San Francisco, 2024.
- 초록/해제
- 요약Ionizing radiation is employed broadly in oncology, but has a narrow therapeutic window arising from nonspecifically induced genomic damage in both healthy and cancerous cells. We employ genome-scale CRISPRi perturb-seq across millions of human cells alongside proliferation screens to profile all expressed genes' effect on heterogenous cellular responses to ionizing radiation in the human chronic myelogenous leukemia cell line K562. We identify undercharacterized radiation sensitizing genes and leverage rich transcriptomic phenotypes to assign putative functional roles based on clustering. We find perturbations which induce radiation-specific responses in single-cell transcriptomic phenotypes like cell cycle state and chromosomal instability, and show how the stressor environment reveals the role of regulatory programs invisible without radiation. A followup validation perturb-seq experiment was used to determine the replicability and broader applicability of these findings, bolstering our confidence in our generation of high-quality transcriptomic profiles to CRISPRi perturbation and ionizing radiation.
- 일반주제명
- Biology
- 일반주제명
- Bioinformatics
- 일반주제명
- Genetics
- 일반주제명
- Cellular biology
- 일반주제명
- Molecular biology
- 키워드
- Perturb-seq
- 키워드
- Human cells
- 키워드
- Phenotypes
- 키워드
- Perturbations
- 기타저자
- University of California, San Francisco Biological and Medical Informatics
- 기본자료저록
- Dissertations Abstracts International. 86-06B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■020 ▼a9798346876786
■035 ▼a(MiAaPQ)AAI31637498
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574
■1001 ▼aWong, Garrett.▼0(orcid)0000-0002-1840-2628
■24510▼aHigh-Information Phenotypic Characterization of the Effect of Ionizing Radiation on Human Cells Using Rich Phenotypes From Perturb-Seq
■260 ▼a[Sl]▼bUniversity of California, San Francisco▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a52 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-06, Section: B.
■500 ▼aIncludes supplementary digital materials.
■500 ▼aAdvisor: Ntranos, Vasilis.
■5021 ▼aThesis (Ph.D.)--University of California, San Francisco, 2024.
■520 ▼aIonizing radiation is employed broadly in oncology, but has a narrow therapeutic window arising from nonspecifically induced genomic damage in both healthy and cancerous cells. We employ genome-scale CRISPRi perturb-seq across millions of human cells alongside proliferation screens to profile all expressed genes' effect on heterogenous cellular responses to ionizing radiation in the human chronic myelogenous leukemia cell line K562. We identify undercharacterized radiation sensitizing genes and leverage rich transcriptomic phenotypes to assign putative functional roles based on clustering. We find perturbations which induce radiation-specific responses in single-cell transcriptomic phenotypes like cell cycle state and chromosomal instability, and show how the stressor environment reveals the role of regulatory programs invisible without radiation. A followup validation perturb-seq experiment was used to determine the replicability and broader applicability of these findings, bolstering our confidence in our generation of high-quality transcriptomic profiles to CRISPRi perturbation and ionizing radiation.
■590 ▼aSchool code: 0034.
■650 4▼aBiology
■650 4▼aBioinformatics
■650 4▼aGenetics
■650 4▼aCellular biology
■650 4▼aMolecular biology
■653 ▼aIonizing radiation
■653 ▼aPerturb-seq
■653 ▼aHuman cells
■653 ▼aPhenotypes
■653 ▼aPerturbations
■690 ▼a0306
■690 ▼a0715
■690 ▼a0369
■690 ▼a0379
■690 ▼a0307
■71020▼aUniversity of California, San Francisco▼bBiological and Medical Informatics.
■7730 ▼tDissertations Abstracts International▼g86-06B.
■790 ▼a0034
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17164715▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
