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Systems-level Readouts of In Vivo ERK Signaling in the Early Drosophila Embryo
Systems-level Readouts of In Vivo ERK Signaling in the Early Drosophila Embryo
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211151324
- ISBN
- 9798382807324
- DDC
- 574
- 저자명
- Zhu, Audrey W.
- 서명/저자
- Systems-level Readouts of In Vivo ERK Signaling in the Early Drosophila Embryo
- 발행사항
- [Sl] : Princeton University, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 105 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 85-12, Section: B.
- 주기사항
- Advisor: Shvartsman, Stanislav Y.;Wuhr, Martin H.
- 학위논문주기
- Thesis (Ph.D.)--Princeton University, 2024.
- 초록/해제
- 요약The highly conserved ERK cascade controls organism development and homeostasis and is genetically deregulated in birth defects, neurocognitive disorders, and cancers. While the list of the ERK functions is vast and steadily growing, the full spectrum of processes controlled by any specific ERK activation event remains unknown. Here we show how new ERK functions can be systematically identified using targeted perturbations and global readouts of ERK activation. Our experimental model is the early Drosophila embryo, where ERK signaling at the embryonic poles has thus far only been associated with transcriptional patterning of the terminal structures of the future larva. Through a combination of imaging and phosphoproteomic analyses of the effects of acute optogenetic perturbations, we demonstrate that ERK activation at the poles is also critical for maintaining the speed and synchrony of embryonic cleavages throughout the embryo. Furthermore, through whole-genome sequencing of ribosome-protected footprints, we set the stage for understanding how ERK signaling affects mRNA translation at this embryonic stage. The presented approaches to interrogating phosphorylation networks, based on global analysis of short-term effects of acute optogenetic stimuli, reveals new functions of an extensively studied signaling event, and sets the stage for comprehensive understanding of signaling systems.
- 일반주제명
- Biology
- 일반주제명
- Biochemistry
- 일반주제명
- Molecular biology
- 일반주제명
- Developmental biology
- 키워드
- Drosophila
- 키워드
- Embryo
- 키워드
- Phosphorylation
- 기타저자
- Princeton University Chemical and Biological Engineering
- 기본자료저록
- Dissertations Abstracts International. 85-12B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■00520250211151324
■006m o d
■007cr#unu||||||||
■020 ▼a9798382807324
■035 ▼a(MiAaPQ)AAI31239815
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574
■1001 ▼aZhu, Audrey W.▼0(orcid)0000-0002-0938-4309
■24510▼aSystems-level Readouts of In Vivo ERK Signaling in the Early Drosophila Embryo
■260 ▼a[Sl]▼bPrinceton University▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a105 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 85-12, Section: B.
■500 ▼aAdvisor: Shvartsman, Stanislav Y.;Wuhr, Martin H.
■5021 ▼aThesis (Ph.D.)--Princeton University, 2024.
■520 ▼aThe highly conserved ERK cascade controls organism development and homeostasis and is genetically deregulated in birth defects, neurocognitive disorders, and cancers. While the list of the ERK functions is vast and steadily growing, the full spectrum of processes controlled by any specific ERK activation event remains unknown. Here we show how new ERK functions can be systematically identified using targeted perturbations and global readouts of ERK activation. Our experimental model is the early Drosophila embryo, where ERK signaling at the embryonic poles has thus far only been associated with transcriptional patterning of the terminal structures of the future larva. Through a combination of imaging and phosphoproteomic analyses of the effects of acute optogenetic perturbations, we demonstrate that ERK activation at the poles is also critical for maintaining the speed and synchrony of embryonic cleavages throughout the embryo. Furthermore, through whole-genome sequencing of ribosome-protected footprints, we set the stage for understanding how ERK signaling affects mRNA translation at this embryonic stage. The presented approaches to interrogating phosphorylation networks, based on global analysis of short-term effects of acute optogenetic stimuli, reveals new functions of an extensively studied signaling event, and sets the stage for comprehensive understanding of signaling systems.
■590 ▼aSchool code: 0181.
■650 4▼aBiology
■650 4▼aBiochemistry
■650 4▼aMolecular biology
■650 4▼aDevelopmental biology
■653 ▼aDrosophila
■653 ▼aRibosome profiling
■653 ▼aExtracellular signal-regulated kinase
■653 ▼aEmbryo
■653 ▼aPhosphorylation
■690 ▼a0306
■690 ▼a0487
■690 ▼a0307
■690 ▼a0758
■71020▼aPrinceton University▼bChemical and Biological Engineering.
■7730 ▼tDissertations Abstracts International▼g85-12B.
■790 ▼a0181
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17161207▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
