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Measuring Messenger RNA Stability Across Developmental Stages and Cell Types Throughout Embryogenesis
Measuring Messenger RNA Stability Across Developmental Stages and Cell Types Throughout Embryogenesis
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211152046
- ISBN
- 9798384025092
- DDC
- 575
- 저자명
- Peng, Felicia.
- 서명/저자
- Measuring Messenger RNA Stability Across Developmental Stages and Cell Types Throughout Embryogenesis
- 발행사항
- [Sl] : University of Pennsylvania, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 122 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 86-02, Section: B.
- 주기사항
- Advisor: Murray, John I.
- 학위논문주기
- Thesis (Ph.D.)--University of Pennsylvania, 2024.
- 초록/해제
- 요약During embryonic development, cells undergo dynamic changes in gene expression that are required for appropriate cell fate specification. Although both transcription and mRNA degradation contribute to gene expression dynamics, patterns of mRNA decay are less well-understood. Here we directly measured spatiotemporally resolved mRNA decay rates transcriptome-wide throughout C. elegans embryogenesis by transcription inhibition followed by bulk and single-cell RNA-sequencing. This allowed us to calculate mRNA half-lives within specific cell types and developmental stages and identify differentially regulated mRNA decay throughout embryonic development. We identified transcript features that are correlated with mRNA stability and found that mRNA decay rates are associated with distinct peaks in gene expression over time. Moreover, we provide evidence that, on average, mRNA is more stable in the germline compared to in the soma and in later embryonic stages compared to in earlier stages. This work suggests that differential mRNA decay across cell states and time helps to shape developmental gene expression, and it provides a valuable resource for studies of mRNA turnover regulatory mechanisms.
- 일반주제명
- Genetics
- 일반주제명
- Cellular biology
- 일반주제명
- Molecular biology
- 일반주제명
- Developmental biology
- 키워드
- mRNA decay
- 키워드
- Embryogenesis
- 키워드
- Cell types
- 기타저자
- University of Pennsylvania Cell and Molecular Biology
- 기본자료저록
- Dissertations Abstracts International. 86-02B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■020 ▼a9798384025092
■035 ▼a(MiAaPQ)AAI31337354
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a575
■1001 ▼aPeng, Felicia.
■24510▼aMeasuring Messenger RNA Stability Across Developmental Stages and Cell Types Throughout Embryogenesis
■260 ▼a[Sl]▼bUniversity of Pennsylvania▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a122 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-02, Section: B.
■500 ▼aAdvisor: Murray, John I.
■5021 ▼aThesis (Ph.D.)--University of Pennsylvania, 2024.
■520 ▼aDuring embryonic development, cells undergo dynamic changes in gene expression that are required for appropriate cell fate specification. Although both transcription and mRNA degradation contribute to gene expression dynamics, patterns of mRNA decay are less well-understood. Here we directly measured spatiotemporally resolved mRNA decay rates transcriptome-wide throughout C. elegans embryogenesis by transcription inhibition followed by bulk and single-cell RNA-sequencing. This allowed us to calculate mRNA half-lives within specific cell types and developmental stages and identify differentially regulated mRNA decay throughout embryonic development. We identified transcript features that are correlated with mRNA stability and found that mRNA decay rates are associated with distinct peaks in gene expression over time. Moreover, we provide evidence that, on average, mRNA is more stable in the germline compared to in the soma and in later embryonic stages compared to in earlier stages. This work suggests that differential mRNA decay across cell states and time helps to shape developmental gene expression, and it provides a valuable resource for studies of mRNA turnover regulatory mechanisms.
■590 ▼aSchool code: 0175.
■650 4▼aGenetics
■650 4▼aCellular biology
■650 4▼aMolecular biology
■650 4▼aDevelopmental biology
■653 ▼amRNA decay
■653 ▼aDevelopmental stages
■653 ▼aEmbryogenesis
■653 ▼aEmbryonic development
■653 ▼aCell types
■690 ▼a0369
■690 ▼a0379
■690 ▼a0758
■690 ▼a0307
■71020▼aUniversity of Pennsylvania▼bCell and Molecular Biology.
■7730 ▼tDissertations Abstracts International▼g86-02B.
■790 ▼a0175
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17162719▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
