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Vascularization of Bioengineered Fat With Tunable Matrix Properties for Macro-Perfusion and Soft Tissue Reconstruction
Vascularization of Bioengineered Fat With Tunable Matrix Properties for Macro-Perfusion and Soft Tissue Reconstruction
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211152656
- ISBN
- 9798384050759
- DDC
- 610
- 서명/저자
- Vascularization of Bioengineered Fat With Tunable Matrix Properties for Macro-Perfusion and Soft Tissue Reconstruction
- 발행사항
- [Sl] : Cornell University, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 281 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 86-03, Section: B.
- 주기사항
- Advisor: Butcher, Jonathan.
- 학위논문주기
- Thesis (Ph.D.)--Cornell University, 2024.
- 초록/해제
- 요약Deep, chronic wounds are a prevalent clinical concern caused by injury or trauma to the skin and underlying soft tissues such as fat, vasculature, fascia, muscle, and even bone. Fat is one of the most abundant and key tissue types necessary for soft tissue reconstruction (STR) and is harvested via autologous fat transfer (AFT), which is the resection and reallocation of adipose tissue from a healthy donor region to the defect site. While these procedures are effective, they are limited by donor-site morbidity, post-operative debilitation, risk of infection and necrosis, and donor tissue availability. 3D-bioprinting and tissue engineering strategies provide a promising solution to address these shortcomings. However, in order to adequately fabricate tissues for regenerative medicine, perfusable and hierarchical vasculature must be incorporated. Additionally, a more robust understanding of matrix mechanics, such as stiffness and viscosity, is crucial to recapitulating the material properties that support fat and vascular formation. Thus, there is a need to establish a tunable system capable of bioengineering fat and other clinically relevant tissues with patent vasculature as an alternative to AFT and other STR procedures. The objective of this work was to examine the vascular and adipogenic potential of endothelial and adipose stem cells within a tunable matrix for macro-perfusion and STR. First, we synthesized and utilized a mechanically tunable bioink, gelatin methacryloyl, to examine the role of matrix stiffness and viscosity on adipogenesis and vasculogenesis. Next, we developed a macro-perfusion bioreactor (MPB) system that can support the fabrication of large constructs with patent and high-throughput lumen geometry (the Squiggle). Finally, we build upon our MPB system, harboring the Squiggle channel design, to elucidate the impact of hemodynamic shear stress and vorticity on bulk diffusion and endothelium maturation, which will eventually serve as a platform to study the effects of hemodynamic flow on angiogenesis. Altogether, the hope is that the knowledge gained from this work and the establishment of a model MPB system can be adapted to engineer fat and other heterogenous tissues with patent and hierarchical vasculature for STR and regenerative medicine.
- 일반주제명
- Biomedical engineering
- 일반주제명
- Biomechanics
- 일반주제명
- Materials science
- 일반주제명
- Engineering
- 키워드
- Adipogenesis
- 키워드
- Hemodynamics
- 키워드
- Matrix stiffness
- 키워드
- Matrix viscosity
- 키워드
- Vasculogenesis
- 기타저자
- Cornell University Biomedical Engineering
- 기본자료저록
- Dissertations Abstracts International. 86-03B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■00520250211152656
■006m o d
■007cr#unu||||||||
■020 ▼a9798384050759
■035 ▼a(MiAaPQ)AAI31487547
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a610
■1001 ▼aStephens, Chelsea Jane.▼0(orcid)0000-0003-3624-0607
■24510▼aVascularization of Bioengineered Fat With Tunable Matrix Properties for Macro-Perfusion and Soft Tissue Reconstruction
■260 ▼a[Sl]▼bCornell University▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a281 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-03, Section: B.
■500 ▼aAdvisor: Butcher, Jonathan.
■5021 ▼aThesis (Ph.D.)--Cornell University, 2024.
■520 ▼aDeep, chronic wounds are a prevalent clinical concern caused by injury or trauma to the skin and underlying soft tissues such as fat, vasculature, fascia, muscle, and even bone. Fat is one of the most abundant and key tissue types necessary for soft tissue reconstruction (STR) and is harvested via autologous fat transfer (AFT), which is the resection and reallocation of adipose tissue from a healthy donor region to the defect site. While these procedures are effective, they are limited by donor-site morbidity, post-operative debilitation, risk of infection and necrosis, and donor tissue availability. 3D-bioprinting and tissue engineering strategies provide a promising solution to address these shortcomings. However, in order to adequately fabricate tissues for regenerative medicine, perfusable and hierarchical vasculature must be incorporated. Additionally, a more robust understanding of matrix mechanics, such as stiffness and viscosity, is crucial to recapitulating the material properties that support fat and vascular formation. Thus, there is a need to establish a tunable system capable of bioengineering fat and other clinically relevant tissues with patent vasculature as an alternative to AFT and other STR procedures. The objective of this work was to examine the vascular and adipogenic potential of endothelial and adipose stem cells within a tunable matrix for macro-perfusion and STR. First, we synthesized and utilized a mechanically tunable bioink, gelatin methacryloyl, to examine the role of matrix stiffness and viscosity on adipogenesis and vasculogenesis. Next, we developed a macro-perfusion bioreactor (MPB) system that can support the fabrication of large constructs with patent and high-throughput lumen geometry (the Squiggle). Finally, we build upon our MPB system, harboring the Squiggle channel design, to elucidate the impact of hemodynamic shear stress and vorticity on bulk diffusion and endothelium maturation, which will eventually serve as a platform to study the effects of hemodynamic flow on angiogenesis. Altogether, the hope is that the knowledge gained from this work and the establishment of a model MPB system can be adapted to engineer fat and other heterogenous tissues with patent and hierarchical vasculature for STR and regenerative medicine.
■590 ▼aSchool code: 0058.
■650 4▼aBiomedical engineering
■650 4▼aBiomechanics
■650 4▼aMaterials science
■650 4▼aEngineering
■653 ▼aAdipogenesis
■653 ▼aHemodynamics
■653 ▼aMatrix stiffness
■653 ▼aMatrix viscosity
■653 ▼aSoft tissue reconstruction
■653 ▼aVasculogenesis
■690 ▼a0541
■690 ▼a0794
■690 ▼a0648
■690 ▼a0537
■71020▼aCornell University▼bBiomedical Engineering.
■7730 ▼tDissertations Abstracts International▼g86-03B.
■790 ▼a0058
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17163349▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
