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Remote Control of Cell Function Using Temperature as an Input
Remote Control of Cell Function Using Temperature as an Input
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211152704
- ISBN
- 9798384023548
- DDC
- 610
- 저자명
- Benman, William.
- 서명/저자
- Remote Control of Cell Function Using Temperature as an Input
- 발행사항
- [Sl] : University of Pennsylvania, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 106 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 86-02, Section: B.
- 주기사항
- Advisor: Bugaj, Lukasz J.
- 학위논문주기
- Thesis (Ph.D.)--University of Pennsylvania, 2024.
- 초록/해제
- 요약The increasing prevalence of RNA and cell-based therapies such as CAR-T, encapsulated cells, and gene therapies have generated a need to control cell function deep inside the body. However, traditional methods of deep-tissue cell actuation require injection of chemicals which can suffer from poor uptake, clearing, and a lack of spatio/temporal precision. Newer methods such as optogenetics suffer from the inability of light to penetrate tissue beyond a few millimeters. An attractive solution to overcome this challenge is using temperature as an inducer. Temperature can be regulated at distances of 10s of centimeters using safe and clinically approved technology such as focused ultrasound or hot/cold compresses. In this work, we develop a modular protein actuator that responds to small changes in temperature. We used this system to thermally control a wide variety of cellular functions including proteolysis, nuclear shuttling, Ras/Erk signaling, cell size, and cell death. Finally, we demonstrate that these tools can be used to non-invasively, locally activate cell death in cancer xenografts in mice.
- 일반주제명
- Bioengineering
- 일반주제명
- Cellular biology
- 일반주제명
- Oncology
- 일반주제명
- Genetics
- 키워드
- Cell function
- 기타저자
- University of Pennsylvania Bioengineering
- 기본자료저록
- Dissertations Abstracts International. 86-02B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■020 ▼a9798384023548
■035 ▼a(MiAaPQ)AAI31488208
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a610
■1001 ▼aBenman, William.
■24510▼aRemote Control of Cell Function Using Temperature as an Input
■260 ▼a[Sl]▼bUniversity of Pennsylvania▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a106 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-02, Section: B.
■500 ▼aAdvisor: Bugaj, Lukasz J.
■5021 ▼aThesis (Ph.D.)--University of Pennsylvania, 2024.
■520 ▼aThe increasing prevalence of RNA and cell-based therapies such as CAR-T, encapsulated cells, and gene therapies have generated a need to control cell function deep inside the body. However, traditional methods of deep-tissue cell actuation require injection of chemicals which can suffer from poor uptake, clearing, and a lack of spatio/temporal precision. Newer methods such as optogenetics suffer from the inability of light to penetrate tissue beyond a few millimeters. An attractive solution to overcome this challenge is using temperature as an inducer. Temperature can be regulated at distances of 10s of centimeters using safe and clinically approved technology such as focused ultrasound or hot/cold compresses. In this work, we develop a modular protein actuator that responds to small changes in temperature. We used this system to thermally control a wide variety of cellular functions including proteolysis, nuclear shuttling, Ras/Erk signaling, cell size, and cell death. Finally, we demonstrate that these tools can be used to non-invasively, locally activate cell death in cancer xenografts in mice.
■590 ▼aSchool code: 0175.
■650 4▼aBioengineering
■650 4▼aCellular biology
■650 4▼aOncology
■650 4▼aGenetics
■653 ▼aEncapsulated cells
■653 ▼aCell-based therapies
■653 ▼aCell function
■653 ▼aCancer xenografts
■690 ▼a0202
■690 ▼a0379
■690 ▼a0992
■690 ▼a0369
■71020▼aUniversity of Pennsylvania▼bBioengineering.
■7730 ▼tDissertations Abstracts International▼g86-02B.
■790 ▼a0175
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17163409▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
