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Advancing Technologies to Study RNA and Its Protein Interactome
Advancing Technologies to Study RNA and Its Protein Interactome
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211153039
- ISBN
- 9798346571483
- DDC
- 540
- 서명/저자
- Advancing Technologies to Study RNA and Its Protein Interactome
- 발행사항
- [Sl] : The University of Wisconsin - Madison, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 221 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 86-05, Section: B.
- 주기사항
- Advisor: Smith, Lloyd M.
- 학위논문주기
- Thesis (Ph.D.)--The University of Wisconsin - Madison, 2024.
- 초록/해제
- 요약Ribonucleic acid or RNA is one of the fundamental building blocks of life. It plays a critical role in the flow of information in the cell by serving as a temporary copy of a gene which can be transported from the nucleus, where the genome is located, into the cytoplasm. Here, the RNA is used to build proteins, which are traditionally considered the main actors of the cell. However, RNA's functions extend beyond a temporary copy of a part of the genome. RNA is involved in nearly every critical process in the cell including transcription, splicing, protein synthesis, posttranscriptional gene regulation, and protein regulation. These complex functions are governed not only by the sequence of the RNA, but also by the proteins it interacts with and chemical modifications to its bases. Therefore, a comprehensive understanding of a given RNA's role within cells requires characterizing both its complete sequence including posttranscriptional modifications as well as its interactors. This level of analysis presents many technical challenges and requires the development of new approaches and expansion of existing strategies. In Chapter 1, an overview of RNA is presented including its coding and noncoding roles in cells, its role in viral infection, the importance of protein interactors, and the significance of posttranscriptional RNA modifications. Chapter 2 introduces hybridization purification of RNA-protein complexes followed by mass spectrometry (HyPR-MS), a strategy for characterizing the protein interactome of a specific RNA. Specifically, this chapter describes expanding the technology for analysis of RNA-protein interactions in tissue. In Chapter 3, HyPR-MS is used to characterize the specific SARS-CoV-2 subgenomic RNAs of virally infected cells. Capture of these closely related species was achieved by targeting the unique junctions of the RNA for capture, showing never before seen resolution in HyPR-MS. In Chapter 4, HyPR-MS is used in conjunction with virus engineering to identify proteins modulating specific steps of the viral life cycle. Specifically, we were looking at the role of host proteins in the packaging of the hepatitis B RNA genome into capsids. In Chapter 5, the focus is shifted to the development of a mass spectrometry-based pipeline for RNA posttranscriptional modification analysis. Finally, Chapter 6 discusses remaining challenges and future directions for comprehensive characterization of RNAs including modifications and interacting partners.
- 일반주제명
- Chemistry
- 일반주제명
- Biochemistry
- 일반주제명
- Virology
- 일반주제명
- Analytical chemistry
- 일반주제명
- Genetics
- 키워드
- Proteomics
- 키워드
- RNA
- 기타저자
- The University of Wisconsin - Madison Chemistry
- 기본자료저록
- Dissertations Abstracts International. 86-05B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■020 ▼a9798346571483
■035 ▼a(MiAaPQ)AAI31638560
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a540
■1001 ▼aWhitworth, Isabella Travene.
■24510▼aAdvancing Technologies to Study RNA and Its Protein Interactome
■260 ▼a[Sl]▼bThe University of Wisconsin - Madison▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a221 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-05, Section: B.
■500 ▼aAdvisor: Smith, Lloyd M.
■5021 ▼aThesis (Ph.D.)--The University of Wisconsin - Madison, 2024.
■520 ▼aRibonucleic acid or RNA is one of the fundamental building blocks of life. It plays a critical role in the flow of information in the cell by serving as a temporary copy of a gene which can be transported from the nucleus, where the genome is located, into the cytoplasm. Here, the RNA is used to build proteins, which are traditionally considered the main actors of the cell. However, RNA's functions extend beyond a temporary copy of a part of the genome. RNA is involved in nearly every critical process in the cell including transcription, splicing, protein synthesis, posttranscriptional gene regulation, and protein regulation. These complex functions are governed not only by the sequence of the RNA, but also by the proteins it interacts with and chemical modifications to its bases. Therefore, a comprehensive understanding of a given RNA's role within cells requires characterizing both its complete sequence including posttranscriptional modifications as well as its interactors. This level of analysis presents many technical challenges and requires the development of new approaches and expansion of existing strategies. In Chapter 1, an overview of RNA is presented including its coding and noncoding roles in cells, its role in viral infection, the importance of protein interactors, and the significance of posttranscriptional RNA modifications. Chapter 2 introduces hybridization purification of RNA-protein complexes followed by mass spectrometry (HyPR-MS), a strategy for characterizing the protein interactome of a specific RNA. Specifically, this chapter describes expanding the technology for analysis of RNA-protein interactions in tissue. In Chapter 3, HyPR-MS is used to characterize the specific SARS-CoV-2 subgenomic RNAs of virally infected cells. Capture of these closely related species was achieved by targeting the unique junctions of the RNA for capture, showing never before seen resolution in HyPR-MS. In Chapter 4, HyPR-MS is used in conjunction with virus engineering to identify proteins modulating specific steps of the viral life cycle. Specifically, we were looking at the role of host proteins in the packaging of the hepatitis B RNA genome into capsids. In Chapter 5, the focus is shifted to the development of a mass spectrometry-based pipeline for RNA posttranscriptional modification analysis. Finally, Chapter 6 discusses remaining challenges and future directions for comprehensive characterization of RNAs including modifications and interacting partners.
■590 ▼aSchool code: 0262.
■650 4▼aChemistry
■650 4▼aBiochemistry
■650 4▼aVirology
■650 4▼aAnalytical chemistry
■650 4▼aGenetics
■653 ▼aHost-pathogen interactions
■653 ▼aPosttranscriptional modifications
■653 ▼aProteomics
■653 ▼aRNA
■653 ▼aRNA -protein interactions
■653 ▼aTechnology development
■690 ▼a0485
■690 ▼a0487
■690 ▼a0720
■690 ▼a0486
■690 ▼a0369
■71020▼aThe University of Wisconsin - Madison▼bChemistry.
■7730 ▼tDissertations Abstracts International▼g86-05B.
■790 ▼a0262
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17164746▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
