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Functional Roles of Chromatin Recruitment to the Nuclear Periphery
Functional Roles of Chromatin Recruitment to the Nuclear Periphery
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211151340
- ISBN
- 9798382812526
- DDC
- 574
- 서명/저자
- Functional Roles of Chromatin Recruitment to the Nuclear Periphery
- 발행사항
- [Sl] : University of California, San Francisco, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 106 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 85-12, Section: B.
- 주기사항
- Advisor: Weiner, Orion.
- 학위논문주기
- Thesis (Ph.D.)--University of California, San Francisco, 2024.
- 초록/해제
- 요약The eukaryotic nucleus houses deoxyribonucleic acid (DNA) along with scaffolding proteins which together form chromatin. Cells organize their chromatin as transcriptionally silent, compacted heterochromatin and active, relaxed euchromatin. Heterochromatin is enriched at the nuclear periphery in metazoans and peripheral position correlates with transcriptional repression. Here, we remove three nuclear lamins and lamin B receptor (LBR) in mouse embryonic stem cells (mESCs) and show that heterochromatin detaches from the nuclear periphery. Heterochromatic loci marked by histone H3 lysine 9 di-methylation (H3K9me2) are enriched at the nuclear periphery; however, the effect of spatial position on H3K9me2-modified genes has not been defined. Chapter 1 is a brief overview of genome organization and the major players studied in this dissertation. Chapter 2 focuses on the effect of heterochromatin spatial position on the transcription and chromatin state of H3K9me2-modified genes in naive mESCs and differentiated Epiblast-like cells (EpiLCs). Chapter 3 shows preliminary work on the effects of heterochromatin spatial position on nuclear shape, on heterochromatin-associated factors such as H3K9me3, HP1α and HP1β, on a degron system to rapidly deplete LBR, and on structure-function rescue experiments to identify regions in LBR that are important for peripheral enrichment of H3K9me2. This work shows that the nuclear periphery controls the spatial position and repressive functions H3K9me2, as well as potentially other heterochromatin marks.
- 일반주제명
- Cellular biology
- 일반주제명
- Molecular biology
- 일반주제명
- Biochemistry
- 키워드
- Nuclear lamina
- 기타저자
- University of California, San Francisco Biochemistry and Molecular Biology
- 기본자료저록
- Dissertations Abstracts International. 85-12B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■00520250211151340
■006m o d
■007cr#unu||||||||
■020 ▼a9798382812526
■035 ▼a(MiAaPQ)AAI31242220
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574
■1001 ▼aMarin, Harold Cesar.▼0(orcid)0000-0001-7691-398X
■24510▼aFunctional Roles of Chromatin Recruitment to the Nuclear Periphery
■260 ▼a[Sl]▼bUniversity of California, San Francisco▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a106 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 85-12, Section: B.
■500 ▼aAdvisor: Weiner, Orion.
■5021 ▼aThesis (Ph.D.)--University of California, San Francisco, 2024.
■520 ▼aThe eukaryotic nucleus houses deoxyribonucleic acid (DNA) along with scaffolding proteins which together form chromatin. Cells organize their chromatin as transcriptionally silent, compacted heterochromatin and active, relaxed euchromatin. Heterochromatin is enriched at the nuclear periphery in metazoans and peripheral position correlates with transcriptional repression. Here, we remove three nuclear lamins and lamin B receptor (LBR) in mouse embryonic stem cells (mESCs) and show that heterochromatin detaches from the nuclear periphery. Heterochromatic loci marked by histone H3 lysine 9 di-methylation (H3K9me2) are enriched at the nuclear periphery; however, the effect of spatial position on H3K9me2-modified genes has not been defined. Chapter 1 is a brief overview of genome organization and the major players studied in this dissertation. Chapter 2 focuses on the effect of heterochromatin spatial position on the transcription and chromatin state of H3K9me2-modified genes in naive mESCs and differentiated Epiblast-like cells (EpiLCs). Chapter 3 shows preliminary work on the effects of heterochromatin spatial position on nuclear shape, on heterochromatin-associated factors such as H3K9me3, HP1α and HP1β, on a degron system to rapidly deplete LBR, and on structure-function rescue experiments to identify regions in LBR that are important for peripheral enrichment of H3K9me2. This work shows that the nuclear periphery controls the spatial position and repressive functions H3K9me2, as well as potentially other heterochromatin marks.
■590 ▼aSchool code: 0034.
■650 4▼aCellular biology
■650 4▼aMolecular biology
■650 4▼aBiochemistry
■653 ▼aCell differentiation
■653 ▼aH3 lysine 9 di-methylation
■653 ▼aNuclear lamina
■653 ▼aNuclear periphery
■653 ▼aDeoxyribonucleic acid
■653 ▼aEpiblast-like cells
■690 ▼a0379
■690 ▼a0487
■690 ▼a0307
■71020▼aUniversity of California, San Francisco▼bBiochemistry and Molecular Biology.
■7730 ▼tDissertations Abstracts International▼g85-12B.
■790 ▼a0034
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17161330▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
