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Combating Gut Pathogens by Precise Virulence Inactivation Using a CRISPR-Associated Transposase
Combating Gut Pathogens by Precise Virulence Inactivation Using a CRISPR-Associated Transposase
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211153124
- ISBN
- 9798346861768
- DDC
- 574
- 서명/저자
- Combating Gut Pathogens by Precise Virulence Inactivation Using a CRISPR-Associated Transposase
- 발행사항
- [Sl] : Columbia University, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 95 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 86-06, Section: B.
- 주기사항
- Advisor: Wang, Harris H.
- 학위논문주기
- Thesis (Ph.D.)--Columbia University, 2024.
- 초록/해제
- 요약Targeted gene manipulation in a complex microbial community is an enabling technology for precise microbiome editing. This thesis introduces a new microbial therapeutic system dubbed Bacterial CRISPR-Transposase Reduction of Virulence In Situ (BACTRINS). BACTRINS is an in-situ microbiome engineering platform designed for efficient and precise genomic insertion of a desired payload and simultaneous knockout of target genes. When applied against a Shiga toxin-producing pathogen in the gut, this system delivers a CRISPR-associated transposase by bacterial conjugation for site-specific inactivation of the Shiga toxin gene and integration of a nanobody therapeutic payload to disrupt pathogen attachment. A single dose of this therapy resulted in high efficiency Shiga gene inactivation and improved survival in a murine infection model of Shiga-producing pathogen. This work establishes a new type of live bacterial therapeutic capable of reducing gut infections by transforming toxigenic pathogens into commensal protectors.
- 일반주제명
- Biology
- 일반주제명
- Microbiology
- 일반주제명
- Pathology
- 일반주제명
- Genetics
- 일반주제명
- Immunology
- 키워드
- Gut pathogens
- 키워드
- Microbiome
- 키워드
- Shiga toxin
- 기타저자
- Columbia University Biological Sciences
- 기본자료저록
- Dissertations Abstracts International. 86-06B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■020 ▼a9798346861768
■035 ▼a(MiAaPQ)AAI31764534
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574
■1001 ▼aPerdue, Tyler David.
■24510▼aCombating Gut Pathogens by Precise Virulence Inactivation Using a CRISPR-Associated Transposase
■260 ▼a[Sl]▼bColumbia University▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a95 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-06, Section: B.
■500 ▼aAdvisor: Wang, Harris H.
■5021 ▼aThesis (Ph.D.)--Columbia University, 2024.
■520 ▼aTargeted gene manipulation in a complex microbial community is an enabling technology for precise microbiome editing. This thesis introduces a new microbial therapeutic system dubbed Bacterial CRISPR-Transposase Reduction of Virulence In Situ (BACTRINS). BACTRINS is an in-situ microbiome engineering platform designed for efficient and precise genomic insertion of a desired payload and simultaneous knockout of target genes. When applied against a Shiga toxin-producing pathogen in the gut, this system delivers a CRISPR-associated transposase by bacterial conjugation for site-specific inactivation of the Shiga toxin gene and integration of a nanobody therapeutic payload to disrupt pathogen attachment. A single dose of this therapy resulted in high efficiency Shiga gene inactivation and improved survival in a murine infection model of Shiga-producing pathogen. This work establishes a new type of live bacterial therapeutic capable of reducing gut infections by transforming toxigenic pathogens into commensal protectors.
■590 ▼aSchool code: 0054.
■650 4▼aBiology
■650 4▼aMicrobiology
■650 4▼aPathology
■650 4▼aGenetics
■650 4▼aImmunology
■653 ▼aGut pathogens
■653 ▼aTargeted gene manipulation
■653 ▼aMicrobiome
■653 ▼aShiga toxin
■653 ▼aBacterial conjugation
■690 ▼a0306
■690 ▼a0410
■690 ▼a0982
■690 ▼a0369
■690 ▼a0571
■71020▼aColumbia University▼bBiological Sciences.
■7730 ▼tDissertations Abstracts International▼g86-06B.
■790 ▼a0054
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17165107▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
