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Stress: Understanding Cellular Adaptation to Environmental Challenges
Stress: Understanding Cellular Adaptation to Environmental Challenges
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211152727
- ISBN
- 9798384484486
- DDC
- 574
- 서명/저자
- Stress: Understanding Cellular Adaptation to Environmental Challenges
- 발행사항
- [Sl] : University of California, Davis, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 213 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 86-04, Section: B.
- 주기사항
- Advisor: Albeck, John G.
- 학위논문주기
- Thesis (Ph.D.)--University of California, Davis, 2024.
- 초록/해제
- 요약During my dissertation in the lab of John Albeck, I have worked on various projects involving measurement of single-cell kinase signaling activity in response to stress using live-cell biosensors. This included optimizing and publishing methodology, the use of live-cell biosensors to measure cellular response to pro-inflammatory cytokines and oxidative stress, and the development of new biosensors. In Chapter 1, I introduce the methods we use to measure signal transduction in real time using fluorescent biosensors, which includes optimizations that I contributed to the lab. This protocol was accepted for publication as a chapter in Methods of Molecular Biology. In Chapter 2, with the help of my co-mentor Amir Zeki, I delve into the application of biosensors and show my finding that spatially coordinated ERK signaling (SPREADs) increased when airway epithelial cells are exposed to pro-inflammatory stimuli like those seen in common airway diseases. I then investigated the potential mechanisms of SPREADs and their suppression by hydrocortisone and metabolic stress that coincides with increased AMPK activity. Chapter 3 focuses on using live-cell microscopy to understand how the RNA-recognition motif of the translation protein eIF4B regulates the cell's ability to form stress granules and suppress protein synthesis following treatment with sodium arsenate. Finally, in Chapter 4, I discuss my work developing, validating, and optimizing Far-Red FRET biosensors for ERK (REKAR67 and REKAR76) and AMPK (RAMPKAR2). Including the use of RAMPKAR2 to understand the single-cell dynamics of AMPK, ATP:ADP ratio, and glycolysis. This last chapter includes work from two different publications in preparation for submission.
- 일반주제명
- Cellular biology
- 일반주제명
- Molecular biology
- 일반주제명
- Oncology
- 일반주제명
- Developmental biology
- 키워드
- Biosensors
- 키워드
- Lung biology
- 기타저자
- University of California, Davis Biochemistry Molecular Cellular and Developmental Biology
- 기본자료저록
- Dissertations Abstracts International. 86-04B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■00520250211152727
■006m o d
■007cr#unu||||||||
■020 ▼a9798384484486
■035 ▼a(MiAaPQ)AAI31490375
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574
■1001 ▼aDeCuzzi, Nicholaus Louis.
■24510▼aStress: Understanding Cellular Adaptation to Environmental Challenges
■260 ▼a[Sl]▼bUniversity of California, Davis▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a213 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-04, Section: B.
■500 ▼aAdvisor: Albeck, John G.
■5021 ▼aThesis (Ph.D.)--University of California, Davis, 2024.
■520 ▼aDuring my dissertation in the lab of John Albeck, I have worked on various projects involving measurement of single-cell kinase signaling activity in response to stress using live-cell biosensors. This included optimizing and publishing methodology, the use of live-cell biosensors to measure cellular response to pro-inflammatory cytokines and oxidative stress, and the development of new biosensors. In Chapter 1, I introduce the methods we use to measure signal transduction in real time using fluorescent biosensors, which includes optimizations that I contributed to the lab. This protocol was accepted for publication as a chapter in Methods of Molecular Biology. In Chapter 2, with the help of my co-mentor Amir Zeki, I delve into the application of biosensors and show my finding that spatially coordinated ERK signaling (SPREADs) increased when airway epithelial cells are exposed to pro-inflammatory stimuli like those seen in common airway diseases. I then investigated the potential mechanisms of SPREADs and their suppression by hydrocortisone and metabolic stress that coincides with increased AMPK activity. Chapter 3 focuses on using live-cell microscopy to understand how the RNA-recognition motif of the translation protein eIF4B regulates the cell's ability to form stress granules and suppress protein synthesis following treatment with sodium arsenate. Finally, in Chapter 4, I discuss my work developing, validating, and optimizing Far-Red FRET biosensors for ERK (REKAR67 and REKAR76) and AMPK (RAMPKAR2). Including the use of RAMPKAR2 to understand the single-cell dynamics of AMPK, ATP:ADP ratio, and glycolysis. This last chapter includes work from two different publications in preparation for submission.
■590 ▼aSchool code: 0029.
■650 4▼aCellular biology
■650 4▼aMolecular biology
■650 4▼aOncology
■650 4▼aDevelopmental biology
■653 ▼aExtracellular signal-regulated kinase
■653 ▼aBiosensors
■653 ▼aFluorescent protein reporters
■653 ▼aForster Resonance Energy Transfer
■653 ▼aLive cell microscopy
■653 ▼aLung biology
■690 ▼a0379
■690 ▼a0307
■690 ▼a0992
■690 ▼a0758
■71020▼aUniversity of California, Davis▼bBiochemistry Molecular Cellular and Developmental Biology.
■7730 ▼tDissertations Abstracts International▼g86-04B.
■790 ▼a0029
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17163585▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
