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Unraveling the Half and Full Site Sequence Specificity of the Saccharomyces cerevisiae Pdr1p and Pdr3p Transcription Factors
Unraveling the Half and Full Site Sequence Specificity of the Saccharomyces cerevisiae Pdr1p and Pdr3p Transcription Factors
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211150918
- ISBN
- 9798381976625
- DDC
- 574.191
- 저자명
- Buechel, Evan R.
- 서명/저자
- Unraveling the Half and Full Site Sequence Specificity of the Saccharomyces cerevisiae Pdr1p and Pdr3p Transcription Factors
- 발행사항
- [Sl] : Northwestern University, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 190 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 85-10, Section: B.
- 주기사항
- Advisor: Pinkett, Heather.
- 학위논문주기
- Thesis (Ph.D.)--Northwestern University, 2024.
- 초록/해제
- 요약The pleiotropic drug resistance (PDR) network is an important regulatory system in Saccharomyces cerevisiae involved in the transport of various toxins and drugs out of the cell. The zinc cluster transcription factors Pdr1p and Pdr3p, key regulators of this network, bind to specific sequences called PDREs within the promoter region of the PDR genes to control expression. However, the exact mechanisms underlying the differences in the regulons of these proteins remain unclear. The focus of this dissertation is on the role of PDRE binding site sequence variation on DNA binding and transcriptional activity of Pdr1p and Pdr3p. A combination of genomic occupancy profiling (CUT&RUN), binding assays and transcription studies were employed to characterize the differences in sequence specificity between the two transcription factors. Distinct preferences for core PDRE sequences and the flanking sequences were revealed for both proteins. While flanking sequences moderately alter DNA binding affinity, they significantly impact Pdr1p/Pdr3p transcriptional activity. Moreover, although Pdr1p and Pdr3p are known to dimerize on PDRE sites, they also have the capacity to bind half sites with high affinity. Transcriptional assays reveal that like PDRE flanking sequences, these half sites appear to modulate transcription from adjacent PDREs. These studies provide insight on how sequence variation within PDREs impacts the function of both Pdr1p and Pdr3p leading to differential regulation within the PDR network.
- 일반주제명
- Biophysics
- 일반주제명
- Biochemistry
- 일반주제명
- Genetics
- 기타저자
- Northwestern University Interdepartmental Biological Sciences (IBiS) Graduate Program
- 기본자료저록
- Dissertations Abstracts International. 85-10B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■00520250211150918
■006m o d
■007cr#unu||||||||
■020 ▼a9798381976625
■035 ▼a(MiAaPQ)AAI30817862
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574.191
■1001 ▼aBuechel, Evan R.▼0(orcid)0000-0002-2134-2582
■24510▼aUnraveling the Half and Full Site Sequence Specificity of the Saccharomyces cerevisiae Pdr1p and Pdr3p Transcription Factors
■260 ▼a[Sl]▼bNorthwestern University▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a190 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 85-10, Section: B.
■500 ▼aAdvisor: Pinkett, Heather.
■5021 ▼aThesis (Ph.D.)--Northwestern University, 2024.
■520 ▼aThe pleiotropic drug resistance (PDR) network is an important regulatory system in Saccharomyces cerevisiae involved in the transport of various toxins and drugs out of the cell. The zinc cluster transcription factors Pdr1p and Pdr3p, key regulators of this network, bind to specific sequences called PDREs within the promoter region of the PDR genes to control expression. However, the exact mechanisms underlying the differences in the regulons of these proteins remain unclear. The focus of this dissertation is on the role of PDRE binding site sequence variation on DNA binding and transcriptional activity of Pdr1p and Pdr3p. A combination of genomic occupancy profiling (CUT&RUN), binding assays and transcription studies were employed to characterize the differences in sequence specificity between the two transcription factors. Distinct preferences for core PDRE sequences and the flanking sequences were revealed for both proteins. While flanking sequences moderately alter DNA binding affinity, they significantly impact Pdr1p/Pdr3p transcriptional activity. Moreover, although Pdr1p and Pdr3p are known to dimerize on PDRE sites, they also have the capacity to bind half sites with high affinity. Transcriptional assays reveal that like PDRE flanking sequences, these half sites appear to modulate transcription from adjacent PDREs. These studies provide insight on how sequence variation within PDREs impacts the function of both Pdr1p and Pdr3p leading to differential regulation within the PDR network.
■590 ▼aSchool code: 0163.
■650 4▼aBiophysics
■650 4▼aBiochemistry
■650 4▼aGenetics
■653 ▼aPleiotropic drug resistance
■653 ▼aSaccharomyces cerevisiae
■653 ▼aTranscription factors
■653 ▼aGenomic occupancy profiling
■690 ▼a0786
■690 ▼a0487
■690 ▼a0369
■71020▼aNorthwestern University▼bInterdepartmental Biological Sciences (IBiS) Graduate Program.
■7730 ▼tDissertations Abstracts International▼g85-10B.
■790 ▼a0163
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17160144▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
