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The Preprophase Band of Microtubules for Division Plane Specification in Land Plants : La Banda de Microtubulos de Preprofase para la Especificacion del Plano de Division en Plantas Terrestres
The Preprophase Band of Microtubules for Division Plane Specification in Land Plants  : La...
The Preprophase Band of Microtubules for Division Plane Specification in Land Plants : La Banda de Microtubulos de Preprofase para la Especificacion del Plano de Division en Plantas Terrestres

상세정보

자료유형  
 학위논문 서양
최종처리일시  
20250211150924
ISBN  
9798382607412
DDC  
574
저자명  
Anleu Gil, Maria Ximena.
서명/저자  
The Preprophase Band of Microtubules for Division Plane Specification in Land Plants : La Banda de Microtubulos de Preprofase para la Especificacion del Plano de Division en Plantas Terrestres
발행사항  
[Sl] : University of California, Davis, 2024
발행사항  
Ann Arbor : ProQuest Dissertations & Theses, 2024
형태사항  
121 p
주기사항  
Source: Dissertations Abstracts International, Volume: 85-11, Section: B.
주기사항  
Includes supplementary digital materials.
주기사항  
Advisor: Liu, Bo.
학위논문주기  
Thesis (Ph.D.)--University of California, Davis, 2024.
초록/해제  
요약The precise spatial control of the division plane is crucial for tissue and organ patterning in plants, and the preprophase band (PPB) is a cytoskeletal structure that regulates division plane orientation in somatic cells. The PPB is primarily composed of bundled cortical microtubules (MTs) with actin and protein components and functions to organize a cortex region that becomes the future division site. The PPB-dependent cue is a positional landmark that guides centrifugal phragmoplast expansion during cytokinesis.Chapter 1 of this thesis offers a comprehensive literature review on the PPB, delving into our current understanding of the molecular mechanisms controlling its formation and function and tracing its evolutionary origins.In Chapter 2, we identify and characterize the IQ Domain (IQD) containing proteins 6/7/8 as microtubule-binding proteins associated with PPB MTs. Triple mutant plants of these IQD genes exhibit oblique cell division planes caused by disorganized PPBs that led to the misspecification of the division plane. Our studies show that robust and solid PPBs are crucial for precise division plane control and that IQD6/7/8 are necessary for properly organizing PPB MTs. Another discovery highlights the cooperation between the MT-polymerase CLIP Associated Protein (CLASP) and IQD6/7/8 in maintaining PPB MT organization and stability. Considering the conserved IQ67 domain in IQD proteins is known to bind calmodulin (CaM) in the presence of calcium (Ca2+), it raises intriguing questions about the role of Ca2+/CaM in division plane regulation.Chapter 3 focuses on the regulation of PPB formation by cyclin and cyclin-dependent kinase (CDK). We confirm that the cyclin A1;1 (CYCA1;1) and CDKA;1 localize at the PPB when expressed under native promoters, revealing an interdependence between CDKA;1 and CYCA1;1 for their PPB localization. However, the specific functions of CDKA;1 and CYCA1;1 at the PPB still need to be clarified. Our findings introduce a connection between cell cycle regulators and the mitotic cytoskeleton machinery potentially influencing PPB emergence or disassembly.In summary, these studies contribute to our understanding of molecular modules that control plant development by studying how plants control their division plane placement. Such knowledge is crucial for understanding how plants build their tissues and organs, which constitute the basis of human food systems.
일반주제명  
Cellular biology
일반주제명  
Plant sciences
일반주제명  
Biology
키워드  
Cyclin-dependent kinase
키워드  
Calcium
키워드  
Preprophase band
키워드  
Microtubules
키워드  
IQ Domain
기타저자  
University of California, Davis Plant Biology
기본자료저록  
Dissertations Abstracts International. 85-11B.
전자적 위치 및 접속  
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MARC

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■00520250211150924
■006m          o    d                
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■020    ▼a9798382607412
■035    ▼a(MiAaPQ)AAI30988265
■040    ▼aMiAaPQ▼cMiAaPQ
■0820  ▼a574
■1001  ▼aAnleu  Gil,  Maria  Ximena.
■24510▼aThe  Preprophase  Band  of  Microtubules  for  Division  Plane  Specification  in  Land  Plants  ▼bLa  Banda  de  Microtubulos  de  Preprofase  para  la  Especificacion  del  Plano  de  Division  en  Plantas  Terrestres
■260    ▼a[Sl]▼bUniversity  of  California,  Davis▼c2024
■260  1▼aAnn  Arbor▼bProQuest  Dissertations  &  Theses▼c2024
■300    ▼a121  p
■500    ▼aSource:  Dissertations  Abstracts  International,  Volume:  85-11,  Section:  B.
■500    ▼aIncludes  supplementary  digital  materials.
■500    ▼aAdvisor:  Liu,  Bo.
■5021  ▼aThesis  (Ph.D.)--University  of  California,  Davis,  2024.
■520    ▼aThe  precise  spatial  control  of  the  division  plane  is  crucial  for  tissue  and  organ  patterning  in  plants,  and  the  preprophase  band  (PPB)  is  a  cytoskeletal  structure  that  regulates  division  plane  orientation  in  somatic  cells.  The  PPB  is  primarily  composed  of  bundled  cortical  microtubules  (MTs)  with  actin  and  protein  components  and  functions  to  organize  a  cortex  region  that  becomes  the  future  division  site.  The  PPB-dependent  cue  is  a  positional  landmark  that  guides  centrifugal  phragmoplast  expansion  during  cytokinesis.Chapter  1  of  this  thesis  offers  a  comprehensive  literature  review  on  the  PPB,  delving  into  our  current  understanding  of  the  molecular  mechanisms  controlling  its  formation  and  function  and  tracing  its  evolutionary  origins.In  Chapter  2,  we  identify  and  characterize  the  IQ  Domain  (IQD)  containing  proteins  6/7/8  as  microtubule-binding  proteins  associated  with  PPB  MTs.  Triple  mutant  plants  of  these  IQD  genes  exhibit  oblique  cell  division  planes  caused  by  disorganized  PPBs  that  led  to  the  misspecification  of  the  division  plane.  Our  studies  show  that  robust  and  solid  PPBs  are  crucial  for  precise  division  plane  control  and  that  IQD6/7/8  are  necessary  for  properly  organizing  PPB  MTs.  Another  discovery  highlights  the  cooperation  between  the  MT-polymerase  CLIP  Associated  Protein  (CLASP)  and  IQD6/7/8  in  maintaining  PPB  MT  organization  and  stability.  Considering  the  conserved  IQ67  domain  in  IQD  proteins  is  known  to  bind  calmodulin  (CaM)  in  the  presence  of  calcium  (Ca2+),  it  raises  intriguing  questions  about  the  role  of  Ca2+/CaM  in  division  plane  regulation.Chapter  3  focuses  on  the  regulation  of  PPB  formation  by  cyclin  and  cyclin-dependent  kinase  (CDK).  We  confirm  that  the  cyclin  A1;1  (CYCA1;1)  and  CDKA;1  localize  at  the  PPB  when  expressed  under  native  promoters,  revealing  an  interdependence  between  CDKA;1  and  CYCA1;1  for  their  PPB  localization.  However,  the  specific  functions  of  CDKA;1  and  CYCA1;1  at  the  PPB  still  need  to  be  clarified.  Our  findings  introduce  a  connection  between  cell  cycle  regulators  and  the  mitotic  cytoskeleton  machinery  potentially  influencing  PPB  emergence  or  disassembly.In  summary,  these  studies  contribute  to  our  understanding  of  molecular  modules  that  control  plant  development  by  studying  how  plants  control  their  division  plane  placement.  Such  knowledge  is  crucial  for  understanding  how  plants  build  their  tissues  and  organs,  which  constitute  the  basis  of  human  food  systems.
■590    ▼aSchool  code:  0029.
■650  4▼aCellular  biology
■650  4▼aPlant  sciences
■650  4▼aBiology
■653    ▼aCyclin-dependent  kinase
■653    ▼aCalcium
■653    ▼aPreprophase  band
■653    ▼aMicrotubules
■653    ▼aIQ  Domain
■690    ▼a0379
■690    ▼a0479
■690    ▼a0306
■71020▼aUniversity  of  California,  Davis▼bPlant  Biology.
■7730  ▼tDissertations  Abstracts  International▼g85-11B.
■790    ▼a0029
■791    ▼aPh.D.
■792    ▼a2024
■793    ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17160165▼nKERIS▼z이  자료의  원문은  한국교육학술정보원에서  제공합니다.

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