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Role of Lipid Transfer Proteins in Lipid Droplet Dynamics
Role of Lipid Transfer Proteins in Lipid Droplet Dynamics
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211150947
- ISBN
- 9798383567067
- DDC
- 574
- 저자명
- Wan, Neng.
- 서명/저자
- Role of Lipid Transfer Proteins in Lipid Droplet Dynamics
- 발행사항
- [Sl] : Yale University, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 107 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 86-02, Section: B.
- 주기사항
- Advisor: Reinisch, Karin M.
- 학위논문주기
- Thesis (Ph.D.)--Yale University, 2024.
- 초록/해제
- 요약Eukaryotic cells compartmentalize their cellular processes in organelles. Lipid droplets (LDs) are key organellesin energy and membrane lipid homeostasis. It has become evident that LDs form membrane contact sites with other organelles throughout their lifetime, from biogenesis to degradation. Lipid transfer proteins (LTPs) localize to LDs, often at sites of LD-organelle contacts. Several known and newly identified LTPs impact LDs biogenesis and degradation, suggesting a role of protein mediated lipid transport in modulating LDs dynamics. The mechanisms of how lipid transport precisely participates in these functions is largely unclear. I characterized the protein spartin, mutations of which leads to neurological disease Troyer syndrome. Spartin localizes to LDs and participates in lipophagy to degrade LDs by tethering LDs close to autophagosome. We hypothesized that spartin might possess lipid transfer activity, like other protein tethers at membrane contact sites. Using biochemical and structural approaches, I discovered that spartin copurifies with phospholipids and neutral lipids from cells and transfers phospholipids in vitro via a so-called senescence domain. A senescence domain truncation impairs lipid transfer in vitro and also impairs LD turnover in cells while not affecting spartin's association with either LDs or autophagosomes, supporting that spartin's lipid transfer ability is physiologically relevant. In summary, my data indicates a novel function of protein mediated lipid transfer in LD degradation, including by lipophagy.
- 일반주제명
- Cellular biology
- 일반주제명
- Molecular biology
- 일반주제명
- Biochemistry
- 일반주제명
- Genetics
- 키워드
- Lipid droplets
- 키워드
- Lipophagy
- 키워드
- Eukaryotic cells
- 기타저자
- Yale University Cell Biology
- 기본자료저록
- Dissertations Abstracts International. 86-02B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■00520250211150947
■006m o d
■007cr#unu||||||||
■020 ▼a9798383567067
■035 ▼a(MiAaPQ)AAI30992662
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574
■1001 ▼aWan, Neng.
■24510▼aRole of Lipid Transfer Proteins in Lipid Droplet Dynamics
■260 ▼a[Sl]▼bYale University▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a107 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-02, Section: B.
■500 ▼aAdvisor: Reinisch, Karin M.
■5021 ▼aThesis (Ph.D.)--Yale University, 2024.
■520 ▼aEukaryotic cells compartmentalize their cellular processes in organelles. Lipid droplets (LDs) are key organellesin energy and membrane lipid homeostasis. It has become evident that LDs form membrane contact sites with other organelles throughout their lifetime, from biogenesis to degradation. Lipid transfer proteins (LTPs) localize to LDs, often at sites of LD-organelle contacts. Several known and newly identified LTPs impact LDs biogenesis and degradation, suggesting a role of protein mediated lipid transport in modulating LDs dynamics. The mechanisms of how lipid transport precisely participates in these functions is largely unclear. I characterized the protein spartin, mutations of which leads to neurological disease Troyer syndrome. Spartin localizes to LDs and participates in lipophagy to degrade LDs by tethering LDs close to autophagosome. We hypothesized that spartin might possess lipid transfer activity, like other protein tethers at membrane contact sites. Using biochemical and structural approaches, I discovered that spartin copurifies with phospholipids and neutral lipids from cells and transfers phospholipids in vitro via a so-called senescence domain. A senescence domain truncation impairs lipid transfer in vitro and also impairs LD turnover in cells while not affecting spartin's association with either LDs or autophagosomes, supporting that spartin's lipid transfer ability is physiologically relevant. In summary, my data indicates a novel function of protein mediated lipid transfer in LD degradation, including by lipophagy.
■590 ▼aSchool code: 0265.
■650 4▼aCellular biology
■650 4▼aMolecular biology
■650 4▼aBiochemistry
■650 4▼aGenetics
■653 ▼aLipid droplets
■653 ▼aLipid trafficking
■653 ▼aLipid transfer proteins
■653 ▼aLipophagy
■653 ▼aEukaryotic cells
■690 ▼a0379
■690 ▼a0487
■690 ▼a0369
■690 ▼a0307
■71020▼aYale University▼bCell Biology.
■7730 ▼tDissertations Abstracts International▼g86-02B.
■790 ▼a0265
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17160273▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
