서브메뉴
검색
Unraveling the Mechanisms Involved in the Drosophila melanogaster Bam Hypomorph Fertility Rescue by Wolbachia pipientis
Unraveling the Mechanisms Involved in the Drosophila melanogaster Bam Hypomorph Fertility Rescue by Wolbachia pipientis
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211150955
- ISBN
- 9798382841137
- DDC
- 575
- 서명/저자
- Unraveling the Mechanisms Involved in the Drosophila melanogaster Bam Hypomorph Fertility Rescue by Wolbachia pipientis
- 발행사항
- [Sl] : Cornell University, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 144 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 85-12, Section: B.
- 주기사항
- Advisor: Aquadro, Charles.
- 학위논문주기
- Thesis (Ph.D.)--Cornell University, 2024.
- 초록/해제
- 요약Bag of marbles (Bam) is a protein known to play a role in germline stem cell (GSC) daughter cell differentiation and self-renewal in female Drosophila melanogaster. Bam interacts with a maternally inherited endosymbiont, Wolbachia pipientis. W. pipientis are known to infect ~40-75% of insects and manipulate the reproduction of their hosts. W. pipientis infection of female bam hypomorphs rescues the mutant fertility phenotype when otherwise the flies would be partially to completely sterile. We aimed to investigate the mechanisms through which W. pipientis rescues the fertility phenotype associated with the bam hypomorph. We determined that W. pipientis in the ovaries and outside of the ovaries correlates with increases in bam hypomorph fertility. Our RNA-seq analysis of the bam hypomorph showed that W. pipientis does not influence bam transcription but influences transcription of bam's genetic and physical interactors. We functionally confirmed the involvement of candidate genes in the bam hypomorph fertility rescue by W. pipientis using genes identified from our RNA-seq analysis and literature. We identified that ubiquitin-associated genes, Ubi-p63E, CycA, otu, and the testis specific transcript, phf7 have novel interactions with W. pipientis and the knockdown of these genes in the bam hypomorph rescued the mutant fertility phenotype. Together, we find that W. pipientis likely modulates the D. melanogaster hosts ubiquitin processes and restores sexual identity to the female germline to rescue bam hypomorph fertility.
- 일반주제명
- Genetics
- 일반주제명
- Microbiology
- 일반주제명
- Cellular biology
- 일반주제명
- Biology
- 키워드
- Bag of marbles
- 키워드
- Oogenesis
- 키워드
- Ubiquitin
- 기타저자
- Cornell University Genetics Genomics and Development
- 기본자료저록
- Dissertations Abstracts International. 85-12B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
008250123s2024 us c eng d■001000017160312
■00520250211150955
■006m o d
■007cr#unu||||||||
■020 ▼a9798382841137
■035 ▼a(MiAaPQ)AAI30993532
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a575
■1001 ▼aKagemann, Catherine Herminia.▼0(orcid)0000-0001-8244-8156
■24510▼aUnraveling the Mechanisms Involved in the Drosophila melanogaster Bam Hypomorph Fertility Rescue by Wolbachia pipientis
■260 ▼a[Sl]▼bCornell University▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a144 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 85-12, Section: B.
■500 ▼aAdvisor: Aquadro, Charles.
■5021 ▼aThesis (Ph.D.)--Cornell University, 2024.
■520 ▼aBag of marbles (Bam) is a protein known to play a role in germline stem cell (GSC) daughter cell differentiation and self-renewal in female Drosophila melanogaster. Bam interacts with a maternally inherited endosymbiont, Wolbachia pipientis. W. pipientis are known to infect ~40-75% of insects and manipulate the reproduction of their hosts. W. pipientis infection of female bam hypomorphs rescues the mutant fertility phenotype when otherwise the flies would be partially to completely sterile. We aimed to investigate the mechanisms through which W. pipientis rescues the fertility phenotype associated with the bam hypomorph. We determined that W. pipientis in the ovaries and outside of the ovaries correlates with increases in bam hypomorph fertility. Our RNA-seq analysis of the bam hypomorph showed that W. pipientis does not influence bam transcription but influences transcription of bam's genetic and physical interactors. We functionally confirmed the involvement of candidate genes in the bam hypomorph fertility rescue by W. pipientis using genes identified from our RNA-seq analysis and literature. We identified that ubiquitin-associated genes, Ubi-p63E, CycA, otu, and the testis specific transcript, phf7 have novel interactions with W. pipientis and the knockdown of these genes in the bam hypomorph rescued the mutant fertility phenotype. Together, we find that W. pipientis likely modulates the D. melanogaster hosts ubiquitin processes and restores sexual identity to the female germline to rescue bam hypomorph fertility.
■590 ▼aSchool code: 0058.
■650 4▼aGenetics
■650 4▼aMicrobiology
■650 4▼aCellular biology
■650 4▼aBiology
■653 ▼aBag of marbles
■653 ▼aOogenesis
■653 ▼aTestis specific transcript
■653 ▼aUbiquitin
■653 ▼aWolbachia pipientis
■690 ▼a0369
■690 ▼a0379
■690 ▼a0410
■690 ▼a0306
■71020▼aCornell University▼bGenetics, Genomics and Development.
■7730 ▼tDissertations Abstracts International▼g85-12B.
■790 ▼a0058
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17160312▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
