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New Insights Into the Neural Circuits That Support Pavlovian Fear Conditioning
New Insights Into the Neural Circuits That Support Pavlovian Fear Conditioning
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211150956
- ISBN
- 9798381949650
- DDC
- 371
- 서명/저자
- New Insights Into the Neural Circuits That Support Pavlovian Fear Conditioning
- 발행사항
- [Sl] : University of California, Los Angeles, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 102 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 85-09, Section: B.
- 주기사항
- Advisor: Adhikari, Avishek.
- 학위논문주기
- Thesis (Ph.D.)--University of California, Los Angeles, 2024.
- 초록/해제
- 요약Pavlovian fear conditioning is the predominant procedure used to study fear learning in rodents. Decades of research has shown that disrupting activity in the basolateral amygdala (BLA) attenuates the acquisition and storage of fear memories (Cousens & Otto, 1998; Maren, Aharonov & Fanselow, 1996; Phillips & LeDoux, 1992). As a result, current models of fear learning posit that information about the stimuli and aversive event converge and are stored in the BLA (Maren & Quirk, 2004; Pitkanen, Savander & LeDoux, 1997). Per this theory, the BLA should be necessary during both the tone and the shock to form this association. To test this theory, experiment 1 took advantage of the temporal specificity of optogenetics and inhibited the BLA during only the tone or only the shock of Pavlovian fear conditioning. The results show that BLA inhibition during the tone, but not the shock, disrupted fear learning and memory, calling into question the necessity of the BLA for processing the shock. Prior experience has a marked effect on future fear learning, but this research primarily focuses on the detrimental effect of stressful experience. The Sharpe lab recently began exploring how prior positive experiences influence fear learning. They found that the lateral hypothalamus (LH) becomes necessary for fear conditioning after reward learning experience (Sharpe et al., 2021). Experiment 2 investigated whether the BLA remain necessary to support fear learning and memory after LH is implicated in fear learning following reward experience. The BLA was optogenetically inhibited during the cue of fear conditioning in rats with or without reward experience. As expected, BLA inhibition disrupted fear memories in naive rats. However, BLA inhibition did not affect fear memories in rats with reward learning experience. Experiment 3 investigated if brief optogenetic inhibition of the BLA in experiment 2 failed to disrupt the BLA at the crucial time to disrupt fear conditioning because the timescale of the BLA's activity shifted. Using chemogenetics, the BLA was inhibited across acquisition of a tone-shock association in rats with or without reward experience. The results of experiment 3 were inconclusive due to insufficient expression of hm4di. Lastly, experiment 4 inhibited the BLA during fear conditioning in reward experienced rats with or without exposure to chronic unpredictable stress (CUS). Rats that received CUS treatment showed enhanced fear learning, but there was no difference in the effect of BLA inhibition during fear learning. Overall, these experiments demonstrate a new perspective on the canonical theory of fear learning and the BLA and expand on how the newfound role of LH in fear learning affects the importance of the BLA for this process and the importance of considering prior experience in our models of the neural circuits that drive learning and memory.
- 일반주제명
- Behavioral psychology
- 일반주제명
- Neurosciences
- 일반주제명
- Psychology
- 키워드
- Neural circuits
- 기타저자
- University of California, Los Angeles Psychology 0780
- 기본자료저록
- Dissertations Abstracts International. 85-09B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■00520250211150956
■006m o d
■007cr#unu||||||||
■020 ▼a9798381949650
■035 ▼a(MiAaPQ)AAI30993628
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a371
■1001 ▼aDiFazio, Lauren Elizabeth.
■24510▼aNew Insights Into the Neural Circuits That Support Pavlovian Fear Conditioning
■260 ▼a[Sl]▼bUniversity of California, Los Angeles▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a102 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 85-09, Section: B.
■500 ▼aAdvisor: Adhikari, Avishek.
■5021 ▼aThesis (Ph.D.)--University of California, Los Angeles, 2024.
■520 ▼aPavlovian fear conditioning is the predominant procedure used to study fear learning in rodents. Decades of research has shown that disrupting activity in the basolateral amygdala (BLA) attenuates the acquisition and storage of fear memories (Cousens & Otto, 1998; Maren, Aharonov & Fanselow, 1996; Phillips & LeDoux, 1992). As a result, current models of fear learning posit that information about the stimuli and aversive event converge and are stored in the BLA (Maren & Quirk, 2004; Pitkanen, Savander & LeDoux, 1997). Per this theory, the BLA should be necessary during both the tone and the shock to form this association. To test this theory, experiment 1 took advantage of the temporal specificity of optogenetics and inhibited the BLA during only the tone or only the shock of Pavlovian fear conditioning. The results show that BLA inhibition during the tone, but not the shock, disrupted fear learning and memory, calling into question the necessity of the BLA for processing the shock. Prior experience has a marked effect on future fear learning, but this research primarily focuses on the detrimental effect of stressful experience. The Sharpe lab recently began exploring how prior positive experiences influence fear learning. They found that the lateral hypothalamus (LH) becomes necessary for fear conditioning after reward learning experience (Sharpe et al., 2021). Experiment 2 investigated whether the BLA remain necessary to support fear learning and memory after LH is implicated in fear learning following reward experience. The BLA was optogenetically inhibited during the cue of fear conditioning in rats with or without reward experience. As expected, BLA inhibition disrupted fear memories in naive rats. However, BLA inhibition did not affect fear memories in rats with reward learning experience. Experiment 3 investigated if brief optogenetic inhibition of the BLA in experiment 2 failed to disrupt the BLA at the crucial time to disrupt fear conditioning because the timescale of the BLA's activity shifted. Using chemogenetics, the BLA was inhibited across acquisition of a tone-shock association in rats with or without reward experience. The results of experiment 3 were inconclusive due to insufficient expression of hm4di. Lastly, experiment 4 inhibited the BLA during fear conditioning in reward experienced rats with or without exposure to chronic unpredictable stress (CUS). Rats that received CUS treatment showed enhanced fear learning, but there was no difference in the effect of BLA inhibition during fear learning. Overall, these experiments demonstrate a new perspective on the canonical theory of fear learning and the BLA and expand on how the newfound role of LH in fear learning affects the importance of the BLA for this process and the importance of considering prior experience in our models of the neural circuits that drive learning and memory.
■590 ▼aSchool code: 0031.
■650 4▼aBehavioral psychology
■650 4▼aNeurosciences
■650 4▼aPsychology
■653 ▼aBasolateral amygdala
■653 ▼aFear conditioning
■653 ▼aLateral hypothalamus
■653 ▼aLearning experience
■653 ▼aNeural circuits
■690 ▼a0384
■690 ▼a0317
■690 ▼a0621
■71020▼aUniversity of California, Los Angeles▼bPsychology 0780.
■7730 ▼tDissertations Abstracts International▼g85-09B.
■790 ▼a0031
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17160321▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
