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Technology Development of High-Resolution Single-Cell Lineage Tracing for Cell Reprogramming Systems
Technology Development of High-Resolution Single-Cell Lineage Tracing for Cell Reprogramming Systems
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211153127
- ISBN
- 9798346860648
- DDC
- 574
- 저자명
- VanHorn, Sadie.
- 서명/저자
- Technology Development of High-Resolution Single-Cell Lineage Tracing for Cell Reprogramming Systems
- 발행사항
- [Sl] : Washington University in St Louis, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 239 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 86-06, Section: B.
- 주기사항
- Advisor: Morris, Samantha A.;Theunissen, Thorold.
- 학위논문주기
- Thesis (Ph.D.)--Washington University in St. Louis, 2024.
- 초록/해제
- 요약A critical element of cell identity is the cell that created the observed cell. This parent cell dictates many aspects of its identity, whether measured by function, phenotype, or specific assays. The cell's lineage impacts the ability of the cell to differentiate into final cell types in development or successfully reprogram in direct cell reprogramming. Here, we evolve molecular barcoding techniques to increase the resolution of lineage recording at single-cell resolution. Using single-cell RNA-sequencing as the readout for this measurement, polyadenylated reporter gene transcripts with engineered 3' untranslated regions were designed to initially label and then record lineage information. While reporter genes with nucleotide "barcode" libraries have become common, we developed an editable region adjacent to this barcode that is targeted with a novel AIDx-dCas12a base editor. This base editor creates single base transitions on the editable region, enabling information to be written over time. We validated this technology in cell lines to illustrate the resolution of the technology and applied it in cell reprogramming to exemplify how lineage information can contribute to our understanding of biology.
- 일반주제명
- Molecular biology
- 일반주제명
- Developmental biology
- 일반주제명
- Genetics
- 키워드
- Lineage tracing
- 기타저자
- Washington University in St. Louis Biology & Biomedical Sciences (Developmental Regenerative & Stem Cell Biology)
- 기본자료저록
- Dissertations Abstracts International. 86-06B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■020 ▼a9798346860648
■035 ▼a(MiAaPQ)AAI31766113
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574
■1001 ▼aVanHorn, Sadie.▼0(orcid)0000-0003-4826-3574
■24510▼aTechnology Development of High-Resolution Single-Cell Lineage Tracing for Cell Reprogramming Systems
■260 ▼a[Sl]▼bWashington University in St Louis▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a239 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-06, Section: B.
■500 ▼aAdvisor: Morris, Samantha A.;Theunissen, Thorold.
■5021 ▼aThesis (Ph.D.)--Washington University in St. Louis, 2024.
■520 ▼aA critical element of cell identity is the cell that created the observed cell. This parent cell dictates many aspects of its identity, whether measured by function, phenotype, or specific assays. The cell's lineage impacts the ability of the cell to differentiate into final cell types in development or successfully reprogram in direct cell reprogramming. Here, we evolve molecular barcoding techniques to increase the resolution of lineage recording at single-cell resolution. Using single-cell RNA-sequencing as the readout for this measurement, polyadenylated reporter gene transcripts with engineered 3' untranslated regions were designed to initially label and then record lineage information. While reporter genes with nucleotide "barcode" libraries have become common, we developed an editable region adjacent to this barcode that is targeted with a novel AIDx-dCas12a base editor. This base editor creates single base transitions on the editable region, enabling information to be written over time. We validated this technology in cell lines to illustrate the resolution of the technology and applied it in cell reprogramming to exemplify how lineage information can contribute to our understanding of biology.
■590 ▼aSchool code: 0252.
■650 4▼aMolecular biology
■650 4▼aDevelopmental biology
■650 4▼aGenetics
■653 ▼aCell reprogramming
■653 ▼aLineage tracing
■653 ▼aSingle-cell resolution
■653 ▼aTechnology development
■690 ▼a0307
■690 ▼a0758
■690 ▼a0369
■71020▼aWashington University in St. Louis▼bBiology & Biomedical Sciences (Developmental, Regenerative, & Stem Cell Biology).
■7730 ▼tDissertations Abstracts International▼g86-06B.
■790 ▼a0252
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17165133▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
