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Development of Magnetic Assays for Quantification of Serum Proteins and Enzymatic Activity
Development of Magnetic Assays for Quantification of Serum Proteins and Enzymatic Activity
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211150912
- ISBN
- 9798383168189
- DDC
- 610
- 서명/저자
- Development of Magnetic Assays for Quantification of Serum Proteins and Enzymatic Activity
- 발행사항
- [Sl] : University of California, San Diego, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 102 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 85-12, Section: B.
- 주기사항
- Advisor: O'Donoghue, Anthony;Hall, Drew;Fraley, Stephanie.
- 학위논문주기
- Thesis (Ph.D.)--University of California, San Diego, 2024.
- 초록/해제
- 요약This research focused on using giant magnetoresistive (GMR) biosensors for point-of-care testing to improve disease diagnosis and monitoring, thereby aiding healthcare decision-making. With their ability to detect subtle changes in local magnetic fields, GMR sensors offer a multiplexed and highly sensitive solution for various biomedical applications. By optimizing the surface chemistry for stable and reproducible conjugation of various biomolecules, the versatile platform enabled specific protein binding, including antibodies for Insulin-like Growth Factor Binding Protein-4 (IBP4) and Sex Hormone Binding Globulin (SHBG), as well as double-stranded DNA substrates for nuclease activity quantitation and protease substrates for quantitation of proteases such as papain and human neutrophil elastase. This work resulted in proof-of-concept of a sensitive, multiplexed, and point-of-care assay for predicting spontaneous preterm birth in pregnant mothers, critical as the complications posed by preterm birth (delivery before 37 weeks of pregnancy) are a leading cause of newborn morbidity and mortality. The assays were validated against mass spectrometry, showing high agreement. These assays have the potential to revolutionize prenatal care by providing timely and precise information to healthcare providers, ultimately improving outcomes for both mothers and infants. We expanded the capabilities of GMR sensors to multiplexed hydrolase quantification, including proteases and nucleases in biological samples. This development is vital for diseases marked by recurrent bacterial lung infections, such as chronic obstructive pulmonary disease and cystic fibrosis. This work highlights the transformative potential of GMR sensors in offering rapid and comprehensive insights into patients' health to enhance healthcare decision-making and improve patient outcomes.
- 일반주제명
- Bioengineering
- 일반주제명
- Biomedical engineering
- 일반주제명
- Biochemistry
- 키워드
- Biosensors
- 키워드
- Diagnostics
- 기타저자
- University of California, San Diego Bioengineering
- 기본자료저록
- Dissertations Abstracts International. 85-12B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■00520250211150912
■006m o d
■007cr#unu||||||||
■020 ▼a9798383168189
■035 ▼a(MiAaPQ)AAI30637454
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a610
■1001 ▼aSveiven, Michael.
■24510▼aDevelopment of Magnetic Assays for Quantification of Serum Proteins and Enzymatic Activity
■260 ▼a[Sl]▼bUniversity of California, San Diego▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a102 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 85-12, Section: B.
■500 ▼aAdvisor: O'Donoghue, Anthony;Hall, Drew;Fraley, Stephanie.
■5021 ▼aThesis (Ph.D.)--University of California, San Diego, 2024.
■520 ▼aThis research focused on using giant magnetoresistive (GMR) biosensors for point-of-care testing to improve disease diagnosis and monitoring, thereby aiding healthcare decision-making. With their ability to detect subtle changes in local magnetic fields, GMR sensors offer a multiplexed and highly sensitive solution for various biomedical applications. By optimizing the surface chemistry for stable and reproducible conjugation of various biomolecules, the versatile platform enabled specific protein binding, including antibodies for Insulin-like Growth Factor Binding Protein-4 (IBP4) and Sex Hormone Binding Globulin (SHBG), as well as double-stranded DNA substrates for nuclease activity quantitation and protease substrates for quantitation of proteases such as papain and human neutrophil elastase. This work resulted in proof-of-concept of a sensitive, multiplexed, and point-of-care assay for predicting spontaneous preterm birth in pregnant mothers, critical as the complications posed by preterm birth (delivery before 37 weeks of pregnancy) are a leading cause of newborn morbidity and mortality. The assays were validated against mass spectrometry, showing high agreement. These assays have the potential to revolutionize prenatal care by providing timely and precise information to healthcare providers, ultimately improving outcomes for both mothers and infants. We expanded the capabilities of GMR sensors to multiplexed hydrolase quantification, including proteases and nucleases in biological samples. This development is vital for diseases marked by recurrent bacterial lung infections, such as chronic obstructive pulmonary disease and cystic fibrosis. This work highlights the transformative potential of GMR sensors in offering rapid and comprehensive insights into patients' health to enhance healthcare decision-making and improve patient outcomes.
■590 ▼aSchool code: 0033.
■650 4▼aBioengineering
■650 4▼aBiomedical engineering
■650 4▼aBiochemistry
■653 ▼aBiosensors
■653 ▼aDiagnostics
■653 ▼aDisease monitoring
■653 ▼aGiant magnetoresistance
■653 ▼aPoint-of-care testing
■690 ▼a0202
■690 ▼a0541
■690 ▼a0487
■690 ▼a0769
■71020▼aUniversity of California, San Diego▼bBioengineering.
■7730 ▼tDissertations Abstracts International▼g85-12B.
■790 ▼a0033
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17160119▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
