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Investigating the Sequence Requirements for Specific Ligand Recognition in Variant Riboswitches
Investigating the Sequence Requirements for Specific Ligand Recognition in Variant Riboswitches
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211150924
- ISBN
- 9798383482230
- DDC
- 574
- 서명/저자
- Investigating the Sequence Requirements for Specific Ligand Recognition in Variant Riboswitches
- 발행사항
- [Sl] : Yale University, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 167 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 86-01, Section: B.
- 주기사항
- Advisor: Strobel, Scott A.
- 학위논문주기
- Thesis (Ph.D.)--Yale University, 2024.
- 초록/해제
- 요약Recognizing and responding to environmental stimuli is essential for organisms across all domains of life. Riboswitches sense environmental stimuli in the form of a small molecule ligand and then structurally rearrange to modulate gene expression in response. Specific ligand recognition is, therefore, a requirement of a functional riboswitch. This dissertation focuses on relating the primary sequence of a riboswitch to a role in ligand specificity. Unlike structural studies, which are used to identify nucleotides in direct ligand contact, I have taken a mutational approach to determine which nucleotides, when mutated, alter riboswitch function or ligand specificity. I focus on variant riboswitches where a small number of sequence changes can change the ligand specificity. I seek to understand how specificity is achieved in closely related variant systems. Peripheral nucleotides and the expression platform both impact ligand specificity in variant riboswitches as well as nucleotides that directly interact with the ligand. In addition to informing on the sequence requirements for specific ligand interaction, this investigation has led to the discovery of a new ykkC variant class as well as the development of potential synthetic pyrimidine-containing cyclic dinucleotide riboswitches.
- 일반주제명
- Biochemistry
- 키워드
- Riboswitch
- 키워드
- Specificity
- 키워드
- Variants
- 기타저자
- Yale University Molecular Biophysics and Biochemistry
- 기본자료저록
- Dissertations Abstracts International. 86-01B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■020 ▼a9798383482230
■035 ▼a(MiAaPQ)AAI30988396
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a574
■1001 ▼aBarth, Kathryn Marie.
■24510▼aInvestigating the Sequence Requirements for Specific Ligand Recognition in Variant Riboswitches
■260 ▼a[Sl]▼bYale University▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a167 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-01, Section: B.
■500 ▼aAdvisor: Strobel, Scott A.
■5021 ▼aThesis (Ph.D.)--Yale University, 2024.
■520 ▼aRecognizing and responding to environmental stimuli is essential for organisms across all domains of life. Riboswitches sense environmental stimuli in the form of a small molecule ligand and then structurally rearrange to modulate gene expression in response. Specific ligand recognition is, therefore, a requirement of a functional riboswitch. This dissertation focuses on relating the primary sequence of a riboswitch to a role in ligand specificity. Unlike structural studies, which are used to identify nucleotides in direct ligand contact, I have taken a mutational approach to determine which nucleotides, when mutated, alter riboswitch function or ligand specificity. I focus on variant riboswitches where a small number of sequence changes can change the ligand specificity. I seek to understand how specificity is achieved in closely related variant systems. Peripheral nucleotides and the expression platform both impact ligand specificity in variant riboswitches as well as nucleotides that directly interact with the ligand. In addition to informing on the sequence requirements for specific ligand interaction, this investigation has led to the discovery of a new ykkC variant class as well as the development of potential synthetic pyrimidine-containing cyclic dinucleotide riboswitches.
■590 ▼aSchool code: 0265.
■650 4▼aBiochemistry
■653 ▼aRiboswitch
■653 ▼aSpecificity
■653 ▼aVariants
■690 ▼a0487
■71020▼aYale University▼bMolecular Biophysics and Biochemistry.
■7730 ▼tDissertations Abstracts International▼g86-01B.
■790 ▼a0265
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17160167▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
