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Inorganic Carbon and Nitrogen Exchange Mechanisms of Cnidarian-Algal Photosymbioses
Inorganic Carbon and Nitrogen Exchange Mechanisms of Cnidarian-Algal Photosymbioses
상세정보
- 자료유형
- 학위논문 서양
- 최종처리일시
- 20250211152139
- ISBN
- 9798384494317
- DDC
- 612
- 서명/저자
- Inorganic Carbon and Nitrogen Exchange Mechanisms of Cnidarian-Algal Photosymbioses
- 발행사항
- [Sl] : University of California, San Diego, 2024
- 발행사항
- Ann Arbor : ProQuest Dissertations & Theses, 2024
- 형태사항
- 169 p
- 주기사항
- Source: Dissertations Abstracts International, Volume: 86-04, Section: B.
- 주기사항
- Advisor: Tresguerres, Martin.
- 학위논문주기
- Thesis (Ph.D.)--University of California, San Diego, 2024.
- 초록/해제
- 요약Photosymbioses between cnidarian hosts and Symbiodiniaceae algae enable the success of coral reef ecosystems. Algae remain photosynthetically active in symbiosis capturing light energy to fix CO2, evolve O2, and produce photosynthates which are transferred to hosts as substrate for growth and metabolism. Host cells phagocytose free-living algae and sequester them in arrested phagosomes, termed "symbiosomes," enabling control over symbionts' metabolisms promoting the production of photosynthates while regulating symbiont population growth. The underlying cellular mechanisms are mostly unknown and only a V-type H+-ATPase (VHA)-dependent carbon-concentrating mechanism (CCM) is documented in corals. My thesis sought to identify whether the CCM is widespread in cnidarian photosymbioses, establish the CCM's importance under various environmental conditions, and identify nitrogen transport mechanisms. Chapter 2 describes the cloning of a candidate coral NH3/NH4+ (total ammonia, Tamm) channel (ayRhp1) from Acropora yongei. Heterologous expression in Xenopus oocytes and flux assays determined ayRhp1 is a dual NH3/CO2 gas channel. Immunofluorescence Airyscan confocal microscopy (IACM) revealed ayRhp1 is present in the symbiosome membrane where, together with VHA, it concentrates Tamm in the symbiont's microenvironment. Furthermore, ayRhp1 is preferentially present in the symbiosome membrane during the day; presumably to provide algae with Tamm to ensure photosynthate production during photosynthesis. Chapter 3 tested whether Cassiopea jellyfish, which host their symbiotic algae in motile cells called amoebocytes, employ a CCM like that from coral host cells. Western blotting and AICM revealed that Cassiopea amoebocytes express both VHA and carbonic anhydrase (CA) and that both proteins localized to the symbiosome membrane. Respirometry using VHA and CA inhibitors revealed significant reductions in O2 evolution rate, a proxy for photosynthetic activity, suggesting that host VHA and CA are functionally coupled in the CCM. Chapter 4 explored the prevalence of the CCM in the coral Stylophora pistillata after acclimation to various environmental light conditions. While VHA protein abundance correlated with increased irradiance, AICM found limited symbiosomal VHA and respirometry experiments did not detect functional evidence for CCMs. Instead, VHA was prominently observed in calcifying cells, where it may contribute to pH regulation for biomineralization. Together, my thesis advances our mechanistic understanding of nutrient exchange in cnidarian photosymbioses.
- 일반주제명
- Physiology
- 일반주제명
- Molecular biology
- 일반주제명
- Cellular biology
- 키워드
- Nutrient cycling
- 키워드
- Photosymbiosis
- 기타저자
- University of California, San Diego Scripps Institution of Oceanography
- 기본자료저록
- Dissertations Abstracts International. 86-04B.
- 전자적 위치 및 접속
- 로그인 후 원문을 볼 수 있습니다.
MARC
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■00520250211152139
■006m o d
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■020 ▼a9798384494317
■035 ▼a(MiAaPQ)AAI31484614
■040 ▼aMiAaPQ▼cMiAaPQ
■0820 ▼a612
■1001 ▼aThies, Angus Blacklaw.
■24510▼aInorganic Carbon and Nitrogen Exchange Mechanisms of Cnidarian-Algal Photosymbioses
■260 ▼a[Sl]▼bUniversity of California, San Diego▼c2024
■260 1▼aAnn Arbor▼bProQuest Dissertations & Theses▼c2024
■300 ▼a169 p
■500 ▼aSource: Dissertations Abstracts International, Volume: 86-04, Section: B.
■500 ▼aAdvisor: Tresguerres, Martin.
■5021 ▼aThesis (Ph.D.)--University of California, San Diego, 2024.
■520 ▼aPhotosymbioses between cnidarian hosts and Symbiodiniaceae algae enable the success of coral reef ecosystems. Algae remain photosynthetically active in symbiosis capturing light energy to fix CO2, evolve O2, and produce photosynthates which are transferred to hosts as substrate for growth and metabolism. Host cells phagocytose free-living algae and sequester them in arrested phagosomes, termed "symbiosomes," enabling control over symbionts' metabolisms promoting the production of photosynthates while regulating symbiont population growth. The underlying cellular mechanisms are mostly unknown and only a V-type H+-ATPase (VHA)-dependent carbon-concentrating mechanism (CCM) is documented in corals. My thesis sought to identify whether the CCM is widespread in cnidarian photosymbioses, establish the CCM's importance under various environmental conditions, and identify nitrogen transport mechanisms. Chapter 2 describes the cloning of a candidate coral NH3/NH4+ (total ammonia, Tamm) channel (ayRhp1) from Acropora yongei. Heterologous expression in Xenopus oocytes and flux assays determined ayRhp1 is a dual NH3/CO2 gas channel. Immunofluorescence Airyscan confocal microscopy (IACM) revealed ayRhp1 is present in the symbiosome membrane where, together with VHA, it concentrates Tamm in the symbiont's microenvironment. Furthermore, ayRhp1 is preferentially present in the symbiosome membrane during the day; presumably to provide algae with Tamm to ensure photosynthate production during photosynthesis. Chapter 3 tested whether Cassiopea jellyfish, which host their symbiotic algae in motile cells called amoebocytes, employ a CCM like that from coral host cells. Western blotting and AICM revealed that Cassiopea amoebocytes express both VHA and carbonic anhydrase (CA) and that both proteins localized to the symbiosome membrane. Respirometry using VHA and CA inhibitors revealed significant reductions in O2 evolution rate, a proxy for photosynthetic activity, suggesting that host VHA and CA are functionally coupled in the CCM. Chapter 4 explored the prevalence of the CCM in the coral Stylophora pistillata after acclimation to various environmental light conditions. While VHA protein abundance correlated with increased irradiance, AICM found limited symbiosomal VHA and respirometry experiments did not detect functional evidence for CCMs. Instead, VHA was prominently observed in calcifying cells, where it may contribute to pH regulation for biomineralization. Together, my thesis advances our mechanistic understanding of nutrient exchange in cnidarian photosymbioses.
■590 ▼aSchool code: 0033.
■650 4▼aPhysiology
■650 4▼aMolecular biology
■650 4▼aCellular biology
■653 ▼aCassiopea jellyfish
■653 ▼aCoral cell biology
■653 ▼aNutrient cycling
■653 ▼aPhotosymbiosis
■690 ▼a0719
■690 ▼a0307
■690 ▼a0379
■71020▼aUniversity of California, San Diego▼bScripps Institution of Oceanography.
■7730 ▼tDissertations Abstracts International▼g86-04B.
■790 ▼a0033
■791 ▼aPh.D.
■792 ▼a2024
■793 ▼aEnglish
■85640▼uhttp://www.riss.kr/pdu/ddodLink.do?id=T17163136▼nKERIS▼z이 자료의 원문은 한국교육학술정보원에서 제공합니다.
